酶工程 的英文怎麼說
中文拼音 [gōngchéng]
酶工程
英文
enzyme engineering-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。We pay attention to the technical content of the product, carries on the deep processing to the product, introduces the bacteriolysis enzyme extraction technology of american group company, take the duck ' s egg as the raw material, extract the bacteriolysis enzyme that is widely applied to the medicine, the cosmetics, food anticorrosion and the bio - engineering fields of research, and we may extract 2500 kilograms every year ; we study the technology of maintaining egg product freshness with the shenyang agricultural college ; abandons with the national environmental protection agriculture using the project center, the dalian environmental science design research institute, the chwangho city environmental protection bureau jointly carries on the birds and beasts feed increase ecology conditioner expansion tests, makes zhuanghe city heidao agriculture comprehensive exploitation co., ltd the provincial level science and technology innovation enterprise
莊河市黑島農業綜合開發有限公司在產品開發中注重提高產品的科技含量，對產品進行深加工，引進美國艾蒂集團公司溶菌酶提取技術，以鴨蛋為原料，提取廣泛應用於醫學、化妝品、食品防腐及生物工程研究領域的溶菌酶，年可提取2500公斤；與沈陽農業大學聯合研究蛋品保鮮技術；與國家環保農廢利用工程中心、大連市環境科學設計研究院、莊河市環保局聯合進行禽飼料添加生態調節劑擴大實驗，使黑島農業綜合開發有限公司成為省級科技創新型企業。Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史，大致經歷了死疫苗、活疫苗（照射致弱尾蚴疫苗）和基因工程疫苗等研究階段，產生了一些who / tdr推薦認為很有希望的疫苗候選分子，如谷胱甘肽- s -轉移酶（ gst ） 、副肌球蛋白（ sm97 ） 、照射致弱疫苗抗原5 （ irv - 5 ） 、磷酸丙糖異構酶（ tpi ） 、曼氏血吸蟲膜內在蛋白（ sm23 ）和脂肪酸結合蛋白（ fabp ， sm14 ）等，但其對宿主的保護作用均不甚理想，未能穩定地達到40或以上的保護力水平，因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑，進一步提高其保護力。Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli
Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。Serials of papain products : papain is a protein hydrolysis enzyme which extracted & purified from papaya latex by advanced biochemical technology
木瓜酶系列產品：木瓜酶是利用現代生物工程技術從未成熟番木瓜乳汁中提取出來的一種蛋白水解酶。The results obtained were as follows : the mic of engineered peptide against staphylococcus aureus atcc 25923 ( penicillin sensitive strain ), atcc 29213 ( penicillinase - producing strain ) and atcc baa - 42 ( methicillin resistant strain ) were 1g / ml, 2g / ml, 4g / nil respectively. pi staining showed the staphylococcus aureus treated with the engineered peptide were dead
實驗結果表明:該工程多膚對三種atcc標準菌株atcc25923 （青霉素敏感菌株） 、 atcc29213 （產青霉素酶菌株） 、 atccbaa一42 （耐甲氧西林菌株）的mlc分別為1林留血l 、 2林留ml 、 4林創ml 。All the currently used thrombolytic agents are plasminogen activators, which are very efficient in restoring the blood flow. they can convert plasminogen into plasmin and thus degrade fibrin. despite the widespread use of established thrombolytic agents such as streptokinase, t - pa and u - pa, all these agents suffer from a number of inadequacies including resistance to reperfusion, occurrence of coronary reocclusion and bleeding complications
介紹了國內外溶栓劑蛋白質工程方面的新進展，主要涉及了組織型纖溶酶原激活劑t - pa尿激酶型纖溶酶原激活劑u - pa鏈激酶sk葡萄球菌激酶sak dspa及tsv - pa的研究進展。Reform of industrial enzymes by protein engineering
蛋白質工程在改造工業用酶中的應用New and highly effective strategies for directed enzyme evolution in vitro have been developed in the protein engineering
酶的體外定向進化是蛋白質工程的新策略。The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene. the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it
豇豆胰蛋白酶抑制劑（ cpti ）基因是目前在植物抗蟲基因工程中應用僅次於bt基因的廣譜性抗蟲基因。鑒於它抗害蟲的廣譜性和靶標昆蟲不易對其產生耐受性的優點，轉cpti基因植物得到了迅速的發展。Along with the development of the cytobiology and the molecular biology, and thoroughly research of the biophysics, the biochemistry, the genetics and immunology, it has cultivated the modem biological technology, such al genetic engineering, cellular engineering, enzyme engineering, fermentation engineering and so on, to change biology characteristic to carry on the material transformation, has formed the front biological examination technology : the dna probe, the pcr technology, the molecular mark, the bioluminescence technology, genechip technology and so on the widespread application of these advanced biotechnologies in dairy industry baa impelled the dairying technical transformation, and has been having vital significance to dairy production, research and dairy product security
摘要隨著細胞生物學和分子生物學的發展及對生物物理、生物化學、遺傳學和免疫學研究的深入，培育了基因工程、細胞工程、酶工程、發酵工程等改變生物特性進行物質轉化的現代生物技術，形成了dna探針、 pcr技術、分子標記、生物熒光技術、基因晶元技術等前沿性的生物檢測技術，其在乳品工業中的廣泛應用，推動了乳業的技術變革，對乳品生產、研究和乳品安全意義重大。Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases
研究人員已經利用蛋白質工程和定點突變技術來改變各種醇腈酶的活性位點和底物特異性等。Beyond generating customized catalysts, these studies have helped to elucidate the enzyme ' s structure - function relationship and illuminate its enantioselectivity
這些工程不但生產出了指定的催化劑，還有助於闡明酶的結構作用關系，並且有助於說明這些酶的對應異構體選擇性。This paper reviewed and discussed the application and advances of fermentation engineering, enzyme engineering, cell engineering and gene engineering in flavor and fragrance industry
本文闡述了發酵工程、酶工程、細胞工程和基因工程在香料中的應用，並控討了生物技術在香料中的應用前景。Biotechnology. vocabulary. enzyme engineering
生物工程.詞匯.酶工程Immobilized cells are the core of the enzyme engineering, which advance the enzyme technology level
摘要固定化細胞技術是酶工程的核心技術之一，它將酶工程提高到一個新水平。Modern biotechnology, represented by gene engineering, cell engineering, enzyme engineering, fermentation engineering and protein engineering, is the forefront for scientific innovation in the 21st century. developing ? iological technique and its relevant industry vigorously has become the core of investment and keystone in economic strategy of many countries around the world. a series of preferential policies and regulations have been set down in order to foster and encourage its development
以基因工程、細胞工程、酶工程、發酵工程和蛋白質工程技術為代表的現代生物技術是21世紀世界科技創新的前沿，大力發展生物技術及其產業已成為各國投資發展的核心產業及經濟戰略重點，並制定了一系列優惠的政策和法規，扶植和鼓勵其發展。For example, in 20 centuries 70 time, as the progress of molecular biology, be united in wedlock with project technology photograph, open up biology technology new field : gene recombines technology of cross of technology, pcr technology, dna and technology of protein alignment analysis, element to wait, the birth that promoted gene project, protein project, cell project, enzymatic project to wait and development
例如，在20世紀70年代，隨著分子生物學的進步，與工程技術相結合，開辟了生物技術新領域：基因重組技術、 pcr技術、 dna和蛋白質序列分析技術、分子雜交技術等，促進了基因工程、蛋白質工程、細胞工程、酶工程等的誕生和發展。Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。分享友人