酶處理細胞 的英文怎麼說

中文拼音 [chǔlǐxìbāo]
酶處理細胞 英文
enzyme treated cell
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 處 : 處名詞1 （地方） place 2 （方面; 某一點） part; point 3 （機關或機關里一個部門） department; offi...
  • 理 : Ⅰ名詞1 （物質組織的條紋） texture; grain （in wood skin etc ） 2 （道理;事理） reason; logic; tru...
  • 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
  • 胞 : Ⅰ名詞1 （胞衣） afterbirth2 （同一個國家或民族的人） fellow countryman; compatriot Ⅱ形容詞（同胞...
  • 細胞 : cell; sytes; bioplast; cella; [口語] gene; [生物學] cellule; cellule cellulli cellulo ; cello ; k...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways

    我們發現，低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應，並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降， dna聚合酶譜發生改變，應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活，細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇，細胞信號轉導通路camp - pka - creb和jnk sapk被激活。
  3. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  4. The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks

    生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。
  5. We treat the porcine skin by 0. 25 percent trypsin, 0. 125 % trypsin, 2. 5 u / ml dispase, hypertonic saline or hypertonic saline - trypsin / dispase. we find that after the skin has been incubated in 0. 125 percent trypsin for 24h at 4 ?, the cells in the skin are all disintegrated. there are no significant differentiation between the acellular matrix treated by 0. 125, 0. 25 perlent trypsin, 2. 5 u / ml dispase and hypertonic saline - trypsin / dispase. but the cell ca n ' t be removed by using the hypertonic saline - sds

    本研究通過對0 25胰酶不同脫細胞時間處理、不同濃度胰酶處理、 dispase脫細胞法、 im 、 zm高滲鹽水脫細胞法、高滲鹽水和胰酶或dispase混合脫細胞法的比較確認採用0 12盼胰酶， 4 ， 244 。
  6. The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid

    將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。
  7. Tumor necrosis factor ( tnf ), a cytkine that exerts many pro - atherosclerotic effects in vivo, causes up - regulation of acat - 1 gene expression in human blood monocytes. by luciferase activity assay, tnf - a enhanced acat - 1 p7 promoter activity in thp - 1 monocytic cell line

    細胞因子tnf -即腫瘤壞死因子大量存在於人動脈粥樣硬化斑塊中，本實驗通過熒光素酶活性和rt - pcr測定結果表明， tnf -處理人血單核細胞和單核細胞株thp - 1均增強acat - 1基因p7啟動子活性。
  8. These are usually collected by dustmen ( molecules called “ ubiquitins ” that pick up proteinaceous litter ) before being taken to the cell ' s waste - processing centre ( a structure known to biologists as the “ proteasome ” )

    這些東西一般都在被送到細胞垃圾處理中心（一種被生物學家稱做「水解酶」結構1 ）之前由清道夫（一種叫「降解酶」的分子，他能夠清理其他蛋白垃圾）收集。
  9. Total cellular rna were extracted, 40 micrograms of the total rna were used in the reverse transcription reaction, using superscript ii reverse transcriptase, oligo ( dt ) i8 primers, and cy3 - dctp or cy5 - dctp for the experimental and control group respectively. the labeled cdnas were hybridized to microarrays at 42c for 12 h - 18 h

    提取as _ 2o _ 3作用k562細胞前後的總rna ，用superscript逆轉錄酶逆轉錄成cdna第一鏈，並在逆轉錄的過程中，用cy3 cy5熒光染料分別標記對照組處理組，與自製的k562細胞基因表達譜晶元雜交。
  10. The organization cuts into slices and examines by the in situ pcr, drip protease k 20 ( xl with loomg / ml to digest respectively in pretreatment, increase with normal position positive cell account for total ratio of cell, according to the positive standard cells > 75 %, confirm the lightest digestion time, studying the influence and relationship of different fixation time with protease digesting each other, detecting the mn genotype of the organize slices at the same time

    石蠟切片進行原位pcr檢，預處理分別滴加loom歲血的蛋白酶k20閃消化，以原位擴增顯色后陽性細胞占總細胞的比值> 75 %為標準，確定最適消化時間『 ， ，研究不同固定時間與蛋白酶消化的相互影響和關系，同時檢測石蠟切片的mn基因型。
  11. Secondly, analysis of peroxidase isoenzyme with polyacrylamidedel electrophoresis for was performed in order to investigate the changes of gene expression under sound stimulation. it could be seen from electrophoresis gel that each group had 6 enzyme bands. new enzyme band in pod electrophoretogram was n ' t detected for stressed groups

    此外，在部分實驗組的培養基中加入不同濃度的蛋白質合成抑制劑環己亞胺酮（ chm ）后發現， pod和cat的活性有所降低，暗示著聲波處理使保護酶活性升高的原因可能是聲波處理促進了細胞內酶的合成。
  12. Based on the above results, a method for producing l - alanine from l - aspartic acid using immobilized pseudomonas dacunhae cells was investigated in this thesis. the cells were immobilized with k - carrageenan and treated with two kind of hardening agents

    結果發現，以-卡拉膠作為包埋劑、再經兩種硬化劑處理得到的固定化細胞，具有較高的酶穩定性和回收率。
  13. It improves on drug - mediated telomerase inhibition, because the cancer cell cannot mutate to resist this treatment - - it would have to create a whole enzyme, telomerase, out of thin air

    它改善了藥物介入的端粒酶抑制，因為癌細胞不可能突變來抵抗這種處理它不能無中生有地創造整個端粒酶。
  14. Mitogen - activated protein ( map ) kinase signal transduction cascades are routes through which eukaryotic cells deliver extracellular messages to the cytosol and nucleus, and the increasing evidences showed that mapks are involved in aba -, sa - or h2o2 - signaling respectively. in addition, plant guard cells have been a well - developed model system for understanding how components interact within a signaling network in a single cell

    本實驗在表皮生物分析的基礎上，主要利用顯微注射技術、膜片鉗技術和激光共聚焦顯微技術，運用專一性蛋白激酶抑制劑處理，探索蛋白激酶對蠶豆（ viciafabal . ）氣孔保衛細胞中aba和sa誘導的h _ 2o _ 2產生及其信號轉導影響機理，結果如下： 1
  15. In this study, one strain of avian rotavirus was isolated from a chicken with natural diarrthoea. isolation and cultivation of the rotavirus was attemped by the inoculating on monolayers of marc 145 cells with a suspension of faecal material containing the rotavirus

    本文對發生在山東某地雞流行性腹瀉病例的病原進行了研究。用胰蛋白酶處理病雞的糞便和腸內容物，接種marc145細胞，盲傳數代後分離到一株病毒。
  16. During the culture of the porcine ear skin fibroblasts, the successful rate was increased by selecting culture methods, contrasting donor age and improving culture condition. the rate of living cells in the method of combining trypsin cold treatment with trypsin heat treatment was much higher than that in the method of trypsin heat treatment. the results showed that the method of combining trypsin cold treatment with trypsin heat treatment had less adverse effects on cells than the method of trypsin heat treatment

    在培養豬耳皮膚成纖維細胞時從篩選細胞分離方法、比較供體年齡和改善培養條件這三方面著手提高培養的成功率。實驗中發現胰蛋白酶冷熱處理結合法培養的細胞存活率明顯高於胰蛋白酶熱處理法，說明使用胰蛋白酶冷熱處理結合法對細胞的不利影響較少，細胞產量較高。
  17. In addition, the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well. by using mrna differential display, 5 desiccation sensitive cdnas, 52 desiccation - induced cdnas, 21 up - regulated cdnas, 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained. the cloning, sequencing, homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as g protein, protein kinase, vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica

    對其中5個脫水特異誘導表達牛耳草光合作廠j的脫水保護和復甦機理的cdna （包括可能與復甦能力有關的cdna ）和3個磷酸鹽處理誘導表達的cdna進行克險測序、同源性探測和northern雜交檢測表明，牛耳草脫水過程中誘導表達的基因可能涉及到脫水脅迫的信號轉導「蛋白、蛋白激酶等） 、調節基因的級聯作用（ vp3 ， mad3樣基因等） 、結構基因產物調節細胞結構（包括細胞質膜）在脫水脅迫中的穩定性等。
  18. 5 statistic treatment with spss soft. results after treatment with mms at various ( 0. 01 ~ immol / l ) concentration for 72h, we examined s. cerevisiae s288c cells for dna - damage situation and changes in telomerase activity

    5統計學處理結果將不同濃度的mms （ 0 . 01一1 ~ fl ）作用於釀酒酵母5288c細胞72h后，分別檢測其dna損傷程度及端粒酶活性變化。
  19. 3. the choosing of optimum evaluating methods of immunobiologic activity : if we disposed sheep blood red cells with neuraminic acid enzyme, the e rosettes forming ratio remarkbly increased ; we improved the conventional method of brdu - elisa assay on lymphocyte proliferation induced by cona. the results showed that lymphocyte proliferation induced by pha was better than by cona. 4

    3 、 gpif免疫生物活性評價方法的優化對gpife玫瑰花環實驗在不影響srbc活性的基礎上用神經氨酸酶處理，經此法處理后e玫瑰花環形成率明顯提高；對傳統的cona誘導的人外周血淋巴細胞增殖brdu - elisa實驗，進行了方法學改進。
  20. Protecting effects of p44 42 mapk signal transduction pathway on hepatocytes in ischemic preconditioning

    絲裂原激活蛋白激酶信號通路在肝臟缺血預處理細胞保護效應中的作用
分享友人
分享到 Line

分享到臉書