酶誘導 的英文怎麼說
中文拼音 [yòudǎo]
酶誘導
英文
enzyme induction-
Effect of active component of four types of chinese herbs on aldose reductase activity and non - enzymatic protein glycation in d - galactose induced rats
半乳糖誘導大鼠醛糖還原酶活性和蛋白非酶糖化的作用Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。The global regulator csra of e. coli is a specific mrna - binding protein. csra negatively regulates several metabolic pathways that are induced post - exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism ; positively controls gene expression within the glycolytic pathway ; and also csra modulates the levels of enzymes that participate directly in pep metabolism
Csra是整體調控網路的調控基因，可負調控指數生長後期誘導的一些代謝途徑，包括糖原的生物合成、糖原的分解代謝、糖異生，而對糖酵解的一些重要基因的表達則執行正調控功能， csra也調控直接參與pep代謝的三個酶的活性水平。Removal of the inducer results in immediate cessation of enzyme synthesis.
除去誘導物就會立即終止酶的合成。Effects of urokinase - type plasminogen activator receptor on sperm chemotactic response induced by urokinase - type plasminogen activator in mice
尿激酶受體在尿激酶誘導小鼠精子趨化運動中的作用Objective to induce vancomycin - resistantant s aureus in vitro and to observe the changes of coagulase gene sequences of s. aureus and its relationship with vancomycin resistance
摘要目的研究萬古黴素敏感金黃色葡萄球菌經過體外誘導變成萬古黴素中介耐藥的金黃色葡萄球菌后凝固酶基因序列有無改變。Relationship between activities of induced exo - chitinase and - 1, 3 - glucanase, and resistance of cowpea to uromyces vignae
豇豆銹菌誘導的2種酶活性與抗病性的關系This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting
本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。So it can induce stronger and earlier iga immune responses at almost all mucosal sites than oral route. the way induced by nasal route is similar to which gut associated lymphoid tissue ( galt ) induced
鼻腔含有的蛋白水解酶較少，抗原不易被破壞，鼻腔免疫可以在多個粘膜部位產生siga反應，誘導與胃腸相關淋巴組織（ galt ）形式上相似的粘膜免疫反應。Moreover, they have hi common the localization in the golgi apparatus, the topology of type ii membrane proteins, and the transfer of galactose from udp - galactose onto the c4 - hydroxyl group of glcnac residues
用iptg誘導表達的獲得- 1 ， 4 - gt與gst （谷胱甘肽轉移酶）的融合蛋白，經westernblot驗證與預想的一致。Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan
在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。Purification and induced resistance of pectinases from penicillium oxalicum bzh
2002果膠酶系的純化及其誘導抗病作用It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation
最佳氮源為玉米漿和蛋白腖，最佳比例為2 ： 3 ，最佳濃度為1 . 6 ；加入其它碳源時有助於菌株穩定產酶； 100ml搖瓶的最佳裝液量為15ml ；肌酸、肌氨酸和氯化膽堿都能誘導菌株產酶，其中肌酸誘導產酶的效果最好，而肌酐和尿素不能誘導菌株產酶；誘導物肌酸的最適加入時間為接種培養12小時后，最適加入量為0 . 75 。To investigate the effect of chinese medicine ruanmai jian on hyperplasia - derived thrombin of vsmcs in rabbits
軟脈煎對凝血酶誘導的家兔胸主動脈血管平滑肌細胞增生的影響Inhibition of focal adhesion kinase induces the anoikis of cardiac fibroblasts with rna interference technique
干擾技術沉默黏附斑激酶誘導心臟成纖維細胞失巢凋亡Characterization of porcine hepatocytes transfected with human catalytic subunit of telomerase reverse transcriptase
人端粒酶逆轉錄酶誘導的豬肝細胞初步鑒定The pharmacokinetic profile of lev closely approximates the ideal characteristics expected of an antiepileptic drug, with good bioavailability, linear and time - invariant kinetics, minimal protein binding, and least metabolism
左乙拉西坦幾乎具備了較好的抗癲癇藥物的所有藥動學特性：生物利用度高、線性曲線、低蛋白結合率、無肝酶誘導作用。分享友人