酸性培養基 的英文怎麼說

中文拼音 [suānxìngpéiyǎngjī]
酸性培養基 英文
acid medium
  • 酸 : 酸構詞成分。
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 酸性 : [化學] acidity; acidness; acerbic; acidic property酸性材料 acid material; 酸性促進劑 acid acceler...
  1. Pig blie salts is the sodium salt of a conjugated bile acid made from pig bile, often used in bacteriological culture media as a selective inhibitory agent

    性狀：本品系由豬膽汁精製而成的一種膽汁酸鈉鹽，是微生物培養基中常用的選擇性抑制劑。
  2. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  3. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  4. With 24 c or 4 c, the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus. in the callus from the different explants, the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme, and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus. so in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the salidroside and the activity of enzymes pal, ca4h and tal, and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside

    （ 2 ） 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶（ pal ） 、肉桂酸解氨酶（ ca4h ）和酪氨酸解氨酶（ tal ）這3種酶的活性；不管是葉來源還是莖來源，不管是24培養還是4培養的愈傷組織，在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的；無論是葉來源的愈傷組織還是莖來源的愈傷組織，培養溫度對pal酶的酶活性影響最大，對ca4h酶的酶活性影響最小，對tal酶的酶活性影響視不同的外植體來源而定；在相同外植體來源的愈傷組織中及不同的培養溫度的條件下，或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下，其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。
  5. Sds - page results showed that as to mut + recombinant highest yield was obtained after 4 days inducing and with the culture time prolonged it reduced. pokeweed antiviral protein gene expressed well when methanol concentration reached 10g / l. pokeweed antiviral protein obtained high yield in thin acidic culture medium ( ph6. 0 - 6. 4 ) and its quantity in total mass of secrete protein exceeded 30 %

    Sds - page分析結果表明， mut ~ +組菌株在甲醇誘導第四天後pap在培養液中積累量達到最高水平，延長培養時間會導致產量下降；在10g / l的甲醇濃度誘導下， pap的表達量達到最高；培養基ph值在偏酸性條件下（ 6 . 0 - 6 . 4 ） pap的表達量都維持在較高的水平。
  6. Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly

    方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。
  7. Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "

    W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0
  8. The self - segregation behavior of amphiphilic copolymer on pdl - la scaffold was investigated via fluorescence - labeling technique. the modified scaffold with hydrophilic surface will not only favor the penetration of cell suspension and culture medium, but also provide the microenvironment for the growth of cells with the peo spacer combining amino acid ( rgd ) structure. according the above result, the cytocompatibility test was also performed on pdl - la 3d scaffold modified by amphiphilic copolymer with alkaline amino acid end

    這種親水表面不僅有利於細胞懸液和培養介質的進入，並可以通過peo橋聯的氨基酸（ rgd ）為細胞在三維多孔支架內的生長提供類細胞外基質環境；根據以上結果，本文對堿性氨基酸為peo鏈端基的兩親共聚物-氨基酸類細胞外基質修飾的聚乳酸三維支架進行了細胞相容性的測試。
  9. The activity of plant and microbic phytases depending on the medium ph was studied

    研究了培養基依賴性植物和微生物肌醇六磷酸酶的活性。
  10. The development of theanine biosynthesis by microorganism fermentation and culturing cells of camellia sinensis was summarized in this paper and the possibility of theanine biosynthesis from the gene engineering strain was also discussed

    摘要綜述了近年來應用微生物發酵法、茶樹細胞培養法生物合成茶氨酸的研究進展，討論了構建基因工程菌生物合成茶氨酸的可行性。
  11. Were studied together with the reference strains of recognized rhizobium and bradyrhizobiwn species by performing polyphasic taxonomy, including numerical taxonomy, rep - pcr fingerprinting, 16s rdna pcr - rflp. the result show that : the growth rate of rhizobia isolated from the root nodules of pueraria spp. showed great diversity. ccbau41147 ccbau6110 k ccbau61096 and ccbau61095 were fast - growing strains, the single colony size was bigger than 1mm after 2 days incubated oq yma medium at 28 they can produce acid. the other strains were slow - growing strains, their single colony size was less than 1 mm after 7 days incubated on yma medium at 28. they can produce alkali

    本研究以從我國四川、河南、安徽和湖南等地分離的32株葛藤根瘤菌為研究對象，以20株已知種的根瘤菌為參比菌株，採用數值分類、 rep - pcr指紋分析、 16srdnapcr - rflp指紋分析等現代根瘤菌分類技術，初步研究了葛藤根瘤菌的生物多樣性和分類地位，結果表明：葛藤根瘤菌在生長速率上表現出多樣性，菌株ccbau41147 、 ccbau61096 、 ccbau61101和ccbau61095生長較快， yma培養基上28培養2 - 3天後，單個菌落直徑大於1mm ，具有產酸能力，是快生型葛藤根瘤菌；其餘待測葛藤根瘤菌生長較慢， yma培養基上28培養7天後，單個菌落直徑小於1mm ，具有產堿能力，是慢生型葛藤根瘤菌。
  12. Effects of fibroblast growth factor 18 on synthesis of alkaline phosphatase in bone marrow stem cells of rabbits culturedin vitro

    成纖維細胞生長因子18對體外培養兔骨髓基質幹細胞堿性磷酸酶合成的影響
  13. A total of 168 strains of actinomycetes, of which 23, or 13. 7 %, displaying antitumor activity, was isolated from near - shore marine samples collected at wenchang mangrove, danzhou harbor and yanpu harbor, three isolation methods were employed, which are sds pretreatment, phenol pretreatment, heating pretreatment and potassium dichronate selection culture, and various media were used

    先後從文昌紅樹林、儋州港以及洋浦港採集樣品，採用苯酚、 sds 、加熱三種樣品預處理法和添加重鉻酸鉀的選擇培養法，並選用多種培養基對這些樣品進行了具有抗腫瘤活性海洋放線菌的分離篩選。共分離到168株放線菌，其中23株具有較強的抗腫瘤活性。
  14. Degradability of the isolates on acetic ester compounds was proved according to the increase of biomass and the decrease of substrates in liquid cultures

    該菌對醋酸酯類化合物的降解性是通過在液體培養基內菌體的增加及底物的減少來證實的。
  15. The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium. after being isolated and purified, these strains were inoculated into fermented medium, shaking in 28 c at 220r / min for 5 days, then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2. 5. the result showed there were 24 strains with higher enzymatic activities among the 102 strains, after the rescreening, 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase, of which, enzymatic activity of the strain 14 was the highest, reaching 53. 86u / ml, and it was preliminarilly identified as aspergillus. niger, then numbered as aspergillus. niger 14

    用植酸鈣選擇性平板培養基從土樣中篩選出了102株能產透明圈的菌株，經分離純化后，接入液體發酵培養基， 28 、 220r min發酵5天，在37 、 ph2 . 5條件下用釩鉬酸銨法測定其所產植酸酶的活力，結果顯示，酶活較高的有24株，經再次搖瓶復篩后，酶活大於15u ml且產酶性能穩定的共有7株，其中以14 ~ #菌株的酶活最高，可達53 . 86u ml ，經初步鑒定為黑麴黴，編號為aspergillus . niger14 ~ # 。
  16. In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate. among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing. after fermented for 5days at 28 c and shaking at 220r / min, the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2. 5 or ph5. 5

    主要結果如下： 1植酸酶高產菌株的篩選利用植酸鈣選擇性子板培養基從土樣中篩選出102株酸性植酸酶產生菌，從中挑選出透明圈較大的菌株32株，經分離純化後分別進行搖瓶復篩， 28 、 220r / min發酵5天後，在37 、 ph2 . 5或ph5 . 5條件下用釩鉬酸銨法檢測其酶活，結果發現有3株菌產酶活性較高且產酶性能較為穩定。
  17. In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k

    本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  18. Bap performed more important function than kt in differentiation of tall fescue embryogenic calli, but better results could be achieved with combination of 2mg / l bap and 0. 5mg / l kt. at this cytokinin level, 0. 5mg / l naa was recommended to obtain the highest callus regeneration frequency. plant regeneration could be evidently boosted when embryogenic calli were pre - differentiated on high - osmoticum medium with 60g / l sucrose, and / or when the pre - differentiated compact calli were differentiated on differentiation medium solidified with l0g / l agar

    高羊茅胚性愈傷組織分化時， bap的作用要比kt大，但2mg lbap與0 . 5mg lkt配合可獲得更佳的效果；在該細胞分裂素水平下，生長素naa用0 . 5mg l ，愈傷組織再生率最高；胚性愈傷組織先在含60g l蔗糖的分化培養基上高滲預分化，以及經高滲預分化后的緻密愈傷組織在瓊脂濃度為10g l的分化培養基上分化，能明顯促進愈傷組織的植株再生；在分化培養基中添加脯氨酸導致愈傷組織再生率下降，但同時有減少白化苗再生的趨勢。
  19. It was also noted that irradiation treatment with low dose ( 10gy ) of y rays exerted slight stimulating effects on callus induction and formation of embryogenic calli in particular. production of embryogenic calli was obviously promoted by addition of 0. 1 ~ 0. 2mg / l bap or 2. 5mg / l cuso4 5h2o, or enhancement of sucrose concentration to 60g / l in subculture medium

    在繼代培養基中添加0 . 1 0 . 2mg lbap或2 . 5mg l硫酸銅，或將繼代培養基中蔗糖濃度提高到60g l能促進胚性愈傷組織發生，提高胚性愈傷組織頻率。
  20. The paper summarizes research advances about the developments of somatic embryos in several conifers, which have appeared at home and abroad in recent years, mainly in the effects of medium components in tissue culture and aba on the development of somatic embryos, and cytology, programmed cell death, genes and protein composition related to the development of somatic embryos, and further discusses the developmental mechanisms of somatic embryos and the role of somatic embryos in genetic transformation system

    本文綜述了近年來國內外有關裸子植物中幾種松杉類植物體細胞胚發育過程的研究報道，其中主要涉及培養基成分和脫落酸（ aba ）對體細胞胚發育的影響，以及體細胞胚發育在細胞學、細胞程序性死亡、相關基因和蛋白質組學等方面的研究進展，並進一步討論了松杉類植物體細胞胚的發育機理，以及體細胞胚在遺傳轉化系統中的作用。
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