鏈比菌的 的英文怎麼說

中文拼音 [liànbǐjūnde]
鏈比菌的 英文
streptotrichal
  • 鏈 : Ⅰ名詞1. （鏈子） chain Ⅱ動詞（用鏈栓住） chain; enchain Ⅲ量詞（計量海洋上距離的長度單位） cable length
  • 比 : Ⅰ動詞1 （比較; 較量高下、 長短、距離、好壞等） compare; compete; contrast; match; emulate 2 （比...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 的 : 4次方是 The fourth power of 2 is direction
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. In part, this is due to the fact that most lobar pneumonias are due to streptococcus pneumoniae ( pneumococcus ) and for decades, these have responded well to penicillin therapy so that advanced, severe cases are not seen as frequently

    部分原因是，絕大多數大葉性肺炎由鏈球菌（肺炎球菌）引起，並且數十年來，青霉素治療對其比較有效，因此復雜、嚴重的病例不常見。
  3. So two of them maybe belong to a new genus. according to the results of polyphasic taxonomy, the phylogenetic relationship of thirteen strains were defied that seven strains such as 40001 belong to streptomyces, strain 40009 belong to nocardiopsis, strain 40010 and 40011 belong to micromonospora, strain 40012 belong to streptosporangium ; strain 40007 and 40008 have similar relationship with actinomadura, but their sequence similarity are lower than 97 %, may belong to a new genus

    實驗獲得了13株放線菌16srdna的近乎全序列，將之與genbank中已知序列進行比較，也可以將這13株放線菌劃分為5個類群，菌株40001等7株菌與鏈黴菌屬（ streptomyces ）的菌株進化關系較近，其中菌株40004和波賽鏈黴菌（ streptomycespeucetius ）的序列相似性為98 . 03 ； 40013與拒黴素鏈黴菌（ streptomycesresistomycificus ）的序列相似性為99 . 02 。
  4. Analysis of the sequence revealed that adda resembled nifs of nitrogen - fixing bacteria and dnda. the entire add gene cluster showed 78 % identity to dnd gene cluster from s. lividans

    同源性比較揭示add基因簇的adda基因與固氮酶基因nifs高度同源， add與變鉛青鏈黴菌dnd核苷酸的同一性為78 。
  5. The powder was then applied to a silica gel column chromatography charged with 80 % acetone in water and eluted with the same solvent. after concentrating and drying, relatively pure fr - 008 was obtained. hplc assay a comparison between antibiotic fr - 008 and candicidin has been made by studying the hplc separation profiles of antibiotic fr - 008, candicidin and a mixture of both

    對鏈黴菌fr - 008和灰色鏈黴菌imru3570的rflp分析發現，它們的染色體具有很高同源性； pfge的比較研究發現，這兩個菌株的明顯區別是鏈黴菌fr - 008攜帶有兩個線性質粒，而在灰色鏈黴菌imru3570中則沒有這兩個線性質粒。
  6. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  7. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  8. More recent but rapidly accumulating experimental evidence indicates that bacterial breakdown products of dietary fibre, the short - chain fatty acids ( scfa ), may play a more important role than fibre per se

    最近快速積累的實驗研究資料提示，膳食纖維的細菌分解產物短鏈脂肪酸（ scfa ） ，可能比膳食纖維有更重要的作用。
  9. Comparison of two kinds of diabetes rats models which are respectively caused by streptozotocin and tetraoxypyrimidine

    鏈脲菌素和四氧嘧啶誘導糖尿病大鼠模型的比較
  10. S. lividans mutant strains zx1 ( dnd cluster deleted ) and zx64 ( dnda disrupted ) had pleiotropic mutations including low mel expression and poor sporulation. it was speculated that dnda together with its downstream dna ( 2. 5kb ) might be involved in these two phenotypes because dnda together with its downstream dna could restore normal sporulation and mel expression to zx64, while dndb and dndc had no such effect because lai and la2 showed no obvious difference in these two phenotypes from wild type s. lividans 1326

    另外通過比較這幾個突變株及野生型菌株在產孢和黑色素基因（ mel ）表達方面的差異，推測dnda及其下游區域與變鉛青鏈黴菌的產孢和刺激外源黑色素基因的表達有關，而dndb和dndc則與之無關，因為la1和la2在這兩種表型上與野生型菌株無明顯差異。
  11. After the primary and secondary selection, 7 strains were tested to have higher productivity than the starting strain. of the 7 strains, strains s1 - 007, s1 - 123 and s1 - 018 were proved to produce more maduramicin than the starting strain. compared with 6236ug / ml of the starting strain, the productivity of the above three strains reached 8020 g / ml, 8254 g / mland9175 g / ml respectively

    出發菌株尤馬馬杜拉放線菌s - 1 ，經過鏈黴素抗性篩選，挑取223個菌株，經過初篩和復篩得到7株菌株馬杜黴素的產量高於出發菌株，其中3個菌株s1 - 007 、 s1 - 123和s1 - 018的產素量與出發菌株的6236ug ml相比有較大提高，分別為8020ug ml ， 8254ug ml和9175ug / ml ，尤其是突變菌株s1 - 018的產量比出發菌株高47 。
  12. Also the ci represser was expressed, presumably from its own phage promoter and prevented transcription from pr at low temperature. moreover, s. lividans strains expressing the c. 100 kda pks were different in sporulation and antibiotic production compared to strains without the 2. 7 kb pks gene, suggesting that the pks protein was active in an unknown manner in streptomyces

    表達pks的變鉛青鏈黴菌菌株在孢子形成和抗生素產生方面與沒有2 . 7kbpks基因的菌株相比較是有區別的，因而推測在鏈黴菌中表達的雙功能結構域pks蛋白具有活性。
  13. Using colophony - paraffin ( cp ) embedding tissue section technique and immunohistochemical streptalidin - peroxidase ( sp ) method, we have investigated the distribution of 5 - ht neurons in the brain of camponotus japonicus ( big worker ant ), compared the immunoreactivity with gaba, and primarily discussed the character of 5 - ht distribution. the results show that 5 - ht immunoreactive processes originate from a relatively small number of cell bodies but each neuron has processes over a large volume of the neuropil of the brain

    本實驗採用樹脂石蠟（ cp ）組織包埋切片技術和鏈黴菌抗生物素蛋白-過氧化物酶（ sp ）免疫組織化學方法，研究了5 - ht能神經元在日本弓背蟻（ camponotusjaponicus ）大工蟻腦中的分佈情況，並與gaba的分佈進行了比較，初步探討了5 - ht在各個腦區的分佈規律和特點。
  14. The dnd phenotype was also found in dna of mycobacterium smegmatis mc2155 by pfge. query of the amino acid sequence of dnd cluster from s. lividance to the uncompleted genome sequence of me2155, a homologous gene with a 38 % identity to dnda was found and was tentatively named mddl

    將變鉛青鏈黴菌的dnd基因簇與mc ~ 2155的基因組序列（未測完）進行同源比較后，發現mc ~ 2155中存在dnda的同源基因（暫命名為mddl ） ，且二者在氨基酸水平上的同一性為38 。
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