阻遏因子 的英文怎麼說
中文拼音 [zǔèyīnzi]
阻遏因子
英文
repressor-
As both the recombinant plasmids and the host dh5a had no laciq gene which encode lac represser, the trc promoter was not repressed by lac represser and always active. therefore the afpga could be constitutively expressed without iptg induction in the host dh5. in the shaking flask, the optimum ph and temperature for afpga expression was 7. 0 and 30
兩重組質粒再轉化宿主菌dh5 ,由於重組質粒和宿主菌dh5均不含laci ~ q調控基因,重組菌不產生阻遏蛋白,控製表達的trc啟動子始終處于「開」狀態,因而所得重組菌dh5 pkkfpga和dh5 psmlfpga不需誘導便能組成型表達pga 。
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