限制酶基因 的英文怎麼說

中文拼音 [xiànzhìjīyīn]
限制酶基因 英文
restricted allele
  • 限 : Ⅰ名詞（指定的范圍; 限度） limit; bounds Ⅱ動詞（指定范圍, 不許超過） set a limit; limit; restrict
  • 制 : Ⅰ動詞1 （製造） make; manufacture 2 （擬訂; 規定） draw up; establish 3 （用強力約束; 限定; 管束...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 限制 : place [impose] restrictions on [to]; astrict; restrict; limit; confine; shut down on [upon]: 限制...
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation

    利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。
  3. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  4. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  5. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  6. Based upon the comparison of cyto b gene sequences in 15 deer species downloaded from genbank, a universal primer set l15774 / hsf21 was used as positive control of the template quality, at the meantime, two species specific primer sets df / dr and cf / cr were desgined to identify red deer ( cervus elaphus ), sika deer ( cervus nippori ) and roe deer ( capreolus capreolus ) from other species. a musk deer ( moschus ) specific primer set wf / mr was designed, siberian musk deer ( afoschus moschiferns ) and forest musk desr ( moschus berezovskii ) could be discriminated when restriction endonuclease rsa i was used to cut the pcr products

    經過對來自genbank中的15種鹿類動物的cytob基因序列的比較，用通用引物l15774和hsf21作為模板的質量控制，設計了特異性引物df dr和cf cr來鑒定馬鹿、梅花鹿、狍；設計了麝類特異性引物mf mr ，用限制性內切酶rsa酶切擴增產物來區分原麝和林麝。
  7. Restriction enzymes and dna probes are used to determine the exact sequence, but it is possible to get a rough estimate by analyzing the frequency of recombination between the alleles of linked genes

    雖然可以利用限制性內切酶和dna探針精確的分析出特定序列，但是在分析連鎖基因等位基因間的重組序列時，會出現不準確的估計。
  8. Down - regulation of rice pullulanase gene expression by rna interference

    干擾技術抑制水稻澱粉極限糊精酶基因表達
  9. An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined

    將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。
  10. It indicated that the chinese isolates belong to north american group. two pairs of different primers of orf7 were used to identify the genotype of prrsv isolates in china, and then compared with the reference isolates of north american and european serotype and modified live prrsv vaccine. the results further proved that prrsv prevalent in china belongs to b genetype. combining the restriction enzyme digestion patterns obtained from mini, hindi and sactt, we observed 2 distinct rflp patterns

    在此基礎上，擴增各毒株的orf5基因，用mlu ， hinc和sac限制性內切酶切割orf5基因，通過這3種限制性內切酶獲得了各毒株的orf5基因限制性酶切圖譜，經rflp分析表明國內分離毒株與美洲型強毒株有著相同的rflp圖譜，而與疫苗毒的rflp圖譜存在明顯差異，進一步證明國內分離毒株的基因型屬於美洲型的強毒株。
  11. Abstract : the polymorphism of angiotensinogen gene at position 174 was studied in 90 cases of essential hypertension patients and 109 controls by pcr, restriction enzyme analysis and electrophoresis methods. the results showed the distribution of genetypes in hypertension group was significantly different from that of controls group. this suggested there is a correlation between the variant of agt174 and hypertension

    摘要本文採用pcr 、限制性酶切和電泳分型等方法，分別對90例原發性高血壓患者和109例正常人血管緊張素原基因多態位點agt174進行了檢測，結果表明，高血壓組中三種基因型的分佈與對照組顯著不同，提高該位點變異與原發性高血壓的發生相關。
  12. When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum

    莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。
  13. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  14. It enables a specific gene to be located on a particular restriction enzyme fragment.

    它就能使專一的基因被定位於特定的限制性內切酶切成的片段上。
  15. Poultry do not have the enzymes to correctly break down these non - starch polysaccharides, which limit the digestibility of corn - soybean - based feeds

    由於家禽體內無法分泌非澱粉多糖酶來正常分解這些非澱粉多糖，因而限制了家禽對玉米豆粕基礎日糧的消化率。
  16. Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained

    Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。
  17. I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

    從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。
  18. The insert dna fragments are 7kb and llkb, respectively. two subclones that were designated pgr3h1 and pgr7h1 and can increase glyphosate resistance of e. coli jm109 up to 150mm glyphosate were constructed by subcloning the 2. 4kb and 3. 2 kb hind ? / psti fragments of pgr3 and pgr7 into the corresponding sites of pgem - 3zf and pbluescript. sequence analysis of these two subclones revealed a completely identical 1323bp open reading frame that encodes an epsp synthase

    以pgem - 3zf和pbluescript為載體，利用限制性內切酶hind和pst構建這兩個克隆的亞克隆，從中分別得到兩個草甘膦耐受亞克隆pgr3h1和pgr7h1 ，插入片段各為2 . 4kb和3 . 2kb ，對這兩個亞克隆進行序列分析，發現二者均含有一個核苷酸序列完全相同的完整的epsp合成酶基因? ? aroa ，其核苷酸序列長為1323bp ，推導的epsp合成酶由441個氨基酸組成，兩個亞克隆的草甘膦耐受濃度最大可達150mm 。
  19. A eukaryotic expression vector pcdna3. 1 - cptl was constructed by insert cp77 gene into the vector pcdna3. 1 which is used in nucleic acid immunization. the vector was immuned the balb / c mice by the method intramuscular injection after extracted and purified in great deal. immu - nological reaction was induced by the expression of cptl after the vector entered into the mice body

    本研究通過限制酶將cpti基因片段從載體pbluel3上切下，插入真核表達載體pcdna3 . 1 ，構建了用於核酸免疫的真核表達載體pcdna3 . 1 - cpti ；質粒大量提取和純化后，通過肌肉注射的方法免疫balb c小鼠，基因表達產物刺激小鼠機體產生免疫反應，從而獲得了抗cpti蛋白的抗體。
  20. By comparing restriction maps of plasmids possessed in these clones, 5 clones ( clones 1, 4, 5, 6, and 8 ) were found to contain the same chitinase gene, while the other three clones ( clones 2, 3 and 9 ) contain different chitinase genes one another

    經底物反應和限制性內切酶圖譜分析，確定其中的clone - 1 ， 4 ， 5 ， 6 ， 8含有相同的幾丁質酶基因；而clone - 2 ， 3 ， 5 ， 9四株重組菌則含有不同的幾丁質酶基因。
分享友人
分享到 Line

分享到臉書