雄性不育基因 的英文怎麼說
中文拼音 [xióngxìngbùyùjīyīn]
雄性不育基因
英文
male sterility gene- 雄 : Ⅰ形容詞1 (生物中能產生精細胞的) male 2 (有氣魄的) grand; imposing 3 (強有力的) powerful; mi...
- 性 : Ⅰ名詞1 (性格) nature; character; disposition 2 (性能; 性質) property; quality 3 (性別) sex ...
- 不 : 名詞[書面語] (剁物所用的木墩) a block of wood
- 育 : 育動詞1. (生育) give birth to 2. (養活; 培育) rear; raise; bring up 3. (教育) educate
- 因 : Ⅰ動詞[書面語] (沿襲) follow; carry on Ⅱ介詞1 [書面語] (憑借; 根據) on the basis of; in accord...
- 雄性 : male雄性動物 buck; 雄性化 masculine; 雄性激素 male sex hormone; androgen; 雄性先熟 protandry (動物); proandry
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Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )
獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育,我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片,共207片(基因槍109片,農桿菌98片) 。在含basta0 . 4mg l的篩選培養基上進行篩選,得到抗性葉片181片(基因槍93片,農桿菌88片) 。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件,以hprt為陽性對照,用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析,結果發現,轉錄sry基因的睪丸組織以及雄性胚胎,從受精卵發育到囊胚的過程中,基本上不轉錄xist基因;不轉錄sry基因的雌性卵母細胞和雌性胚胎,從出現原核開始,到發育至2 -細胞期的過程中, xist基因一直不轉錄,但是,從4 -細胞期開始,一直到孵化前囊胚階段,雌性胚胎都轉錄xist基因。Consideration on developing preharvest sprouting resistant rice male sterile line by expressing antisense rna of viviparous
1基因培育水稻抗穗萌雄性不育系的設想Molecular examination and biological observation of transformed plants by growing resistant germinations we got 30 transformed plants. pcr and spot blotting hybridization analysis indicate that hsp70 anti - sense cdna had been integrated into tobacco genome. through observation and measurement we found 12 completely sterile, 9hyper - sterile, 3 partially sterile and 6 fertile and significant differences between them in stamen, height, and fruit weight
Pcr和點雜交分析表明, hsp70正、反義。 dna己經整合到煙草的基因組中。在進行田間生物學性狀觀察時, 30株轉基因植株中有12株表現出完全不育, 9株高度不育, 3株部分不育和6株可育;不育植株在雄蕊、株高和果實重量等方面都存在明顯的差異。Construction of anther specific chimeric promoter and further establishment of transgenic artificial male sterile arabidopsis thaliana
花藥特異嵌合啟動子的構建及雄性不育轉基因擬南芥的獲得In order to select male nucleo - sterile new genotype, a tentative idea was put forward for the nucleo - male sterility to attach a tps ( thermo - photoperiod sensitivity ) and a selection strategy of combination of selection and identification, at the same time, the spring and summer sowing method were used to provide different environment conditions of appraising sterility and tps. the results indicated that ( 1 ) sterility could be appraised under the spring sowing environment and tps could be appraised under summer sowing environment. ( 2 ) under spring sowing environment, sterility could be selected, but not maintained. thereby, lines selected could only be selected as recorded selection method in the experiment. ( 3 ) and then, selection was carried out from spring sowing line selected into summer sowing in same line with tps to select plants. these plants through the intercrossing selection had been combined with sterility and tps. in this way, a new selection protocol for selection sterile line with tps was formed. it mainly involves the spring and summer sowing method, recorded selection method and the intercrossing selection method
為了選育新類型玉米雄性核不育系,提出了為玉米核不育性添加溫光敏感性的設想和選擇與鑒定相結合的策略,同時應用分期播種的方法為作物提供不同的生長和發育的環境條件,以鑒定玉米雄穗的育性變化和對不同環境條件溫光的反應.研究結果表明,春播環境下可鑒定和選擇玉米的不育性,夏播環境下可鑒定和選擇其溫光敏感性.針對玉米核不育性難以找到保持系的特性,結合兩種播期選擇兩種性狀.但春播環境下選擇的不育性群體難以通過選擇單株來保持其不育性,為記錄性群體選擇.通過從春播入選的雜合不育性優良株系群體轉移到其對應的夏播溫光敏感性入選的同一優良群體中進行優良單株選擇,能逐漸使不育性和溫光敏感性相結合而選育出純合溫光敏不育系.這種新的選擇程序主要包括應用分期播種法、記錄性選擇法和春夏兩季交叉式選擇法,使含有不育基因的可育株系逐步累積不育基因並增加了溫光敏感性而育成玉米溫光敏不育系From 19 arabidopsis male sterile lines isolated from an ethyl methanesulphonate - induced ( ems - induced ) population, a total of four male sterile mutants were screened with each mutant controlled by a single recessive gene
首先對19個經化學誘變劑ems處理得到的雄性不育突變體進行背景純化和遺傳分析,從中篩選到四個單個隱性基因控制的雄性不育突變體( ec2 - 157 、 ec1 - 188 、 ec2 - 115和ec2 - 214 ) 。Cloning of rejection related gene and construction of antisense gene transferring vector
玉米溫敏核雄性不育相關基因克隆Effect of cytoplasmic male sterile genes on agronomic and biochemical characters in cms lines and their f1 hybrids in pepper
辣椒細胞質雄性不育基因對不育系及雜交一代農藝性狀和生化特性的影響The technology and methods of the study on molecular mechanism of cytoplasm male sterility ( cms ) are introduced in regard to mitochondrial genome, mitochondrial gene, mitochondrial rna, mitochondrial protein, transformed plants and abortion of pollen
摘要本文從線粒體基因組、線粒體基因、線粒體轉錄rna 、線粒體蛋白、轉基因植物以及花粉敗育機理六個方面詳細介紹了植物細胞質雄性不育分子生物學研究的技術和方法。In the transgenic tobacco plants with sense gene promoted by camv35s, the anthers of four lines were changed into petals partly or totally or no anther at all
Camv35啟動子驅動st901基因在轉基因煙草、馬鈴薯中的表達同樣導致植株的雄性不育,這是通過影響轉基因植株花藥的發育而實現的。Gene cloning differential fragment gha27 was produced by cdna - aflp method employed to compare the gene expression in developing anthers between the male sterile and fertile plants of cotton dong a. a cdna clone, designated gharf ( gossypium hirsutum adp - ribosy lation factor ), was isolated from a cotton ( gossypium hirsutum l. ) cdna library using gha27 as probe. 2
棉花arf基因的克隆選取棉花洞a雄性不育株和可育株花藥cdna - aflp差別顯示的差異片段gha27 ,用地高辛標記作為探針,篩選棉花洞a花藥的cdna文庫,克隆得到了棉花arf基因的cdna全長序列,將其命名為gharf ( gossypiumhirsutumadp - ribosylationfactor ) 。The research progresses of the inheritance of fertility restoration and molecular mapping of restoring genes of cytoplasmic male sterility ( cms ) in rice were summarized, and fine mapping and cloning of restoring genes were also reviewed, which would facilitate the further studies on restoring genes and their application in rice
摘要闡述了水稻細胞質雄性不育及育性恢復的遺傳特點以及恢復基因的分子標記定位研究進展,同時對恢復基因的精細定位與克隆做了簡單介紹,為今後進一步對水稻恢復基因的研究和應用提供參考。Abstract : the progress in studies on induction of male sterili ty and restorer line in plant by gene engineering was reviewed. the prospects on application of this method in heteriosis utilization were discussed
摘要本文對利用基因工程創造植物雄性不育系和恢復系的研究進展進行了綜述,並對其在雜種優勢利用中的應用前景進行了展望。The results indicate that : in both normal temperature and heat shock, the contents of mitochondrion proteins of sterile plants and control plants are significantly different ( p < 0. 01, n = 10 ) ; the differences of contents of mitochondrion proteins of sterile plants in the normal temperature and heat shock response are not significant, which mean that the expression of hsp70 gene is inhibited and the function of mitochondrion is disturbed
01 , n 10 ) ;而不育植株在常溫和熱激條件下線粒體蛋白含量差異不顯著,說明轉化株中hsp70基因的表達受到抑制,從而進一步擾亂了線粒體的功能,最終導致轉化株雄性不育,表明hsp70反義cdna能夠通過抑制hsp70基因的表達導致植物雄性不育。Zhao yin - he, tan xue - lin, li ben - xun. inherence of male sterile and restore and research advance of molecular markers of restoring genes in hybrid rice. crops, 2002 ( 4 ) : 6 8
趙銀河,譚學林,李本遜.水稻雄性不育、恢復性的遺傳及恢復基因的分子標記研究進展.作物雜志, 2002 ( 4 ) : 6 8In this dissertation we focused on the function of genes associated with anther and pollen development including map - based cloning of arabidopsis genes from male sterile mutants
本論文以正向遺傳學的方法研究花藥及花粉發育過程的基因功能,主要利用圖位克隆的方法從擬南芥雄性不育突變體中克隆相應的基因。It was reviewed here that the major advances of biological research on the fertility restoration gene for cytoplasmic male sterility in plant in the past decade
摘要綜述了近10年來植物細胞質雄性不育恢復基因分子生物學的研究進展。Hsp70, a kind of molecular chaperone, has the main functions of taking part in protein folding, protein degredation and reparation of dna damadge and has important effects on the constructure and function of mitochondria. it has already been proved that there is a close correlation between hsp70 and the development of plants and animals. this paper deals with integrating sense and antisense cdnas of hsp70 into tobacco dna by constructing an expression vector of sense and antisense cdnas of hsp70 and gene - transforming methods - genegun bombarding and agrobacterium mediation. provided expression of hsp70 gene is inhibited by sense and antisense cdnas of hsp70 we can get male sterile plants so as to prove that antisense cdna of hsp70 leads to male sterility 1
Hsp70是一種分子伴侶,主要功能是參與細胞有關蛋白新生肽的折疊、亞基組裝、細胞內運輸、蛋白質降解及dna損傷的修復,對線粒體結構和功能發揮重要作用,已有研究證明hsp70與動植物的發育有密切的關系,本研究將hsp70正、反義cdna構建成表達載體,並運用基因槍和農桿菌介導法將hsp70正、反義cdna導入煙草,試通過hsp70反義cdna抑制hsp70基因的表達從而創造雄性不育株,以證明hsp70反義cdna能創造雄性不育系。5 - fu can influence the cell ' s survival through interference with the synthesis metabolism of the rna and dna. the intent of this research is to probe into the possibility of creating chemically controlled on - off male sterile plant lines, by using the characteristic of coda gene, and by consequnce, to establish a plant " two - line " hybrid production platform
5 - fu通過干擾rna和dna的合成代謝從而影響細胞的生存。本研究旨在利用coda基因的這一特性,探討創造植物雄性不育和保持「兼用系」的可能性,以期建立植物「兩系法」雜交制種的技術平臺。分享友人