雌性原核 的英文怎麼說
中文拼音 [cíxìngyuánhé]
雌性原核
英文
thelyblast-
A total of 38 males and 24 females irradiated at the pronuclear stage survived and were available for testing.
在原核期受照中共有38隻雄性和24隻雌性存活下來，並可供試驗。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件，以hprt為陽性對照，用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析，結果發現，轉錄sry基因的睪丸組織以及雄性胚胎，從受精卵發育到囊胚的過程中，基本上不轉錄xist基因；不轉錄sry基因的雌性卵母細胞和雌性胚胎，從出現原核開始，到發育至2 -細胞期的過程中， xist基因一直不轉錄，但是，從4 -細胞期開始，一直到孵化前囊胚階段，雌性胚胎都轉錄xist基因。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。The objective of this study was whether the reconstituted embryo with the second polar body ( pb2 ) can develop. firstly, female mouse was superovulated with pmsg + hcg and keep in the same cage with male mouse, the pronuclear embryo was obtained at the different time after hcg injection with different motheds to investigate the existing time of pronuclear embryo. then pronuclear embryo was cultured with three different mediums to select the best to culture in vitro
母鼠在注射hcg后與公鼠同籠交配，並在注射hcg后不同時間（從18h到26h ） 、不同方法（體內法和體外法）獲取胚胎，研究獲取原核胚的最佳時間；在此基礎上獲得具有第二極體原核胚，然後對原核胚進行顯微操作，去除雌原核並注入第二極體，探討第二極體內遺傳物質支持胚胎發育的潛力；同時利用dna特異性染料h33342對原核胚進行染色，顯示並比較原核和第二極體內的dna遺傳物質。The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。This study were designed to investigate development potency of the reconstructed embryos of black bear - cat inter - species nuclear transfer in vitro / vivo, to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm. somatic nuclear transplantation technology has been used to establish stem cell from different animals, which can provide materials to treat various diseases
本研究運用體細胞核移植的原理和技術，以六月齡雌性黑熊的成纖維細胞為供核體，去核未受精家貓成熟卵母細胞為受體，構建種間核移植重組胚胎，研究種問體細胞核移植胚胎的構建方法和重組胚胎的體內外發育潛能及其影響因素。分享友人