驗證雜交 的英文怎麼說

中文拼音 [yànzhèngjiāo]
驗證雜交 英文
check cross
  • : 動詞1. (察看; 查考) examine; check; test 2. (產生預期的效果) prove effective; produce the expected result
  • : Ⅰ動詞(證明) prove; verify; demonstrate Ⅱ名詞1 (證據) evidence; proof; testimony; witness 2 (...
  • : Ⅰ形容詞(多種多樣的; 混雜的) miscellaneous; varied; sundry; mixed Ⅱ動詞(混合在一起; 攙雜) mix; blend; mingle
  • : Ⅰ動詞1 (把事物轉移給有關方面) hand over; give up; deliver 2 (到某一時辰或季節) reach (a cert...
  • 驗證 : test and verify; checking; proving; testing; confirmation; [數學] corroboration; inspection; veri...
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中,木耳屬2個種的2個菌株在its區域表現出較高的保守性, 4種限制型內切酶的酶切圖譜沒有顯示出多態性;增加內切酶種類及供試菌株數量,有可能獲得具有多態性的限制性內切酶酶切圖譜; 9本實中, its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異,擴增片段長度均為650bp左右; 10根據形態學實、 eric - pcr實以及southern的結果分析,紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法,兩者的關系是相輔相成,互為
  2. ( 2 ) gene flow frequency was reduced as distance from pollen donor increased and a dramatic reduction occurred at about 2 meters. the maximum distance where gene flow was not detected was 50 m for hybrid rice while it was 70 m for ms lines, with an exception that in one of the four ms lines it was detected a frequency of gene flow 2. 8 + 10 - 6 at 150 m for zhong 9a

    在開花期主流風向ne的風速為0 . 2 ? 2 . 2m / sec的條件下, 2個稻品種的最大漂流距離為40m ; 4個不育系的基因漂流基本上到60m為止, 70m處基因漂流頻率均降為0 ,僅中9a在150m處發現了1株basta抗性苗,經pcr檢測為陽性。
  3. Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins

    將4株經southern正確的基因缺失突變株進行搖瓶發酵和hplc檢測,發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分,阿維菌素的總產量和b1的產量與出發菌株相當,說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。
  4. Finally, the hybridization on the 3 - pyrrole - dna polymer modified electrode was detected with methylene blue ( mb ) as a hybridization indicator, thus the function of 3 - pyrrole - dna modified electrode was qualitatively analyzed

    結果明, mb可以用作無標記的dna檢測;同時表明,電聚合法製作的dna晶元是有檢測功能的。
  5. Abstract : the paper considers that the experiment of affect scion of prunus japonica thunb on stock of prunus armeniaca l. furnish fresh evidence for graft hybridization , and proposes the possible mechnism for production of tillering prunus japoniaca thunb according to recent achievements in plant molecular biology

    摘要本文認為「接穗郁李影響杏砧變異的實」為嫁接研究提供了新的據,並根據近年來植物分子生物學的有關研究成果,對蘗生郁李產生的原因進行了解釋。
  6. The results showed that all six tfs displayed no autoluminescence in this two - hybrid system, and one of them, the atf3 displayed a strong interaction with restin. to verify the interaction of restin and atf3, a series of truncated fragments of atf3 were inserted into pact to be used in the further study

    三、為了進一步atf3與restin的相互作用,對atf3進行截斷並構建了一系列atf3的截短片段的雙質粒(仍然構建於pact載體中) ,再次運用細胞雙進行相互作用的探索。
  7. The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced

    首先以中肋骨條藻的rdna序列為設計種特異性引物的靶區域,共設計出7對適合rfq - pcr的引物(依次命名為primer1 ( f r ) primer7 ( f r ) ) ,並用常規pcr實初步明其中有4對引物( primer1 ( f r ) 、 primer2 ( f r ) 、 primer5 ( f r ) 、 primer6 ( f r ) )可作為中肋骨條藻特異性引物的候選者;然後測定了以primer6 ( f r )為引物的pcr產物的序列,序列分析表明,中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大,從中可設計出滿足rfq - pcr需要的taqman探針(命名為taqman6 ) ;進一步的核酸表明, taqman6隻與中肋骨條藻的pcr產物,不與其他藻的pcr產物
  8. Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system

    進一步通過rt . pcr克隆了ked樣蛋白編碼區全長cdna ,通過雙了全長ked樣蛋白與acamz的相互作用,結果為陽性。
  9. Using the complex potential method in the plane theory of elasticity of an anisotropic body, the series solution of finite anisotropic thin plate containing an elliptical inclusion is proposed with the help of faber series. a hybrid element with an elliptical inclusion for anisotropic materials is obtained by using the hybrid variable principle, and the element efficiency is verified by numerical examples. the state of the damage is modeled by an elliptical soft inclusion, and using the point stress criterion based on characteristic curve and yamada - sun etc. criteria, the prediction of the strength of a composite laminate with damage is set up

    首先基於經典層板理論,將復合材料層板的彈性問題化歸為均勻各向異性板來求解;採用各向異性體平面彈性理論中的復勢方法,以faber級數為工具,給出了有限大含橢圓核各向異性板彈性問題的級數解形式;利用變分原理,成功導出含橢圓核各向異性板應力有限元,並用算例了該單元的可行性和有效性;採用含剛度折減橢圓形彈性核的沖擊損傷模型,引入基於特徵曲線和yamada - sun破壞準則的點應力判據,建立了含損傷復合材料層板剩餘強度的分析方法;通過數值計算詳細討論了各種幾何參數對損傷層板應力分佈、剩餘強度的影響,得到了一系列對工程應用具有實用價值的結論。
  10. The main purpose of this paper is to investigate the application of the neuron network for the daily exchange rate forecasting. generalized cross validation is introduced to determine the number of nodes of the hidden layer, several well known time series forecasting methods are also compared with the nn method in this paper

    討論了人工神經網路在金融匯率預報中的應用。其中介紹了廣義generalized cross validation法如何應用於確定神經網路中隱層的個數,並用實例說明了該方法甚至對復的非線性函數也可以得到很好的逼近。
  11. The blotting detection showed : the pcr detection on 35s and nos is correct, while when the gms content decrease the detection signal weakened. so this method was not fit to be applied in market detection

    明pcr擴增的35s啟動子和nos終止子為特異性擴增,當材料轉基因含量降低時,檢測信號則明顯降低。
  12. This paper try to mix the finite strip method and hybrid stress element, and deduce a numerical and computer method which combines the advantages of the two methods - mixed hybrid finite strip method ; analyze the intensity and stability of the orthotropic plate by this method ; deduce the formula of the orthotropic plate ' s analysis and calculation under this method ; compile the program of orthotropic plate ' s mixed hybrid finite strip method ( hfsop. for ) with fortran - 77 language, and perform them under fortran90 environment of pc computer ; comparing with traditional methods by example of computing, prove that the mixed hybrid finite strip method have better degree of accuracy, moderating the stiffness of plate, coming more close to actual conditions, avoiding over - hard or over - soft ; finally, this paper analyze the stability of a orthotropic plate by this method, and deduce the - value of critical stress

    論文將有限條法和應力元法兩者結合起來,導出一種兼有兩者優點的數值計算方法? ?混合有限條法。運用這種方法對正異性板的強度和穩定進行了分析,推導出正異性板在這種方法下的分析計算公式,用fortran - 77語言編寫正異性板混合有限條法程序( hfsop . for )在微機fortran90環境下運行。通過算例與傳統的方法比較,混合有限條法有更好的精度,使板的剛度適中,更接近實際情況,避免「過硬」或「過軟」 ;最後,論文還用此方法對一正異性板進行了穩定分析,得出臨界應力值。
  13. It indicated that the magnetosome deleted phenotype w as caused by tn5 insertion

    Southern磁小體缺失突變確為tn5插入所致。
  14. On the base of trust path and certificate validation steps, we analyze the time complexity of two trust models ( subordinated hierarchy and cross - certified mesh model ), and then analyze the methods of certificate validation in current standards

    第三,介紹了信任路徑的概念,的一般步驟,在此基礎上分析了兩種具有代表性的信任模型(附屬層次模型和叉認網模型)的復度。
  15. Pllz1112 was transformed into m145 to disrupt sc6a9. 34. a mutant named hxy1 was obtained and confirmed to be disrupted at correct site by southern blotting

    將phz1112導入野生型菌株m145中獲得基因中斷菌株hxy1並通過southernhxy1在正確位置發生了基因中斷。
  16. Taking the bending stiffness, cable sag and cable inclination into consideration, the space vibration control of the cables using the visco - elastic dampers in cable - stayed bridges is investigated by joining the center difference method and the state space strategy. both the maximum modal damping ration and the optimal damper size are obtained, then the practical suggestions are proposed for the design of the dampers. the space nonlinear vibration equations of the cable - damper system are derived, and a new hybrid method for solving the cable - damper system is presented by combing the newmark method and pseudo - force technology

    綜合考慮了拉索抗彎剛度、垂度的影響,研究了粘彈性阻尼器對斜拉橋拉索的空間振動控制,聯合中心差分方法及狀態空間法,得出了拉索麵內、外振動各階模態可能達到的最大阻尼比及相應的最優阻尼器系數,並對斜拉橋拉索的阻尼器設計提出了參考建議:考慮拉索抗彎剛度、垂度及幾何非線性,導出了索-阻尼器系統的空間振動非線性方程組,結合newmark方法及偽力( pseudo - force )方法,創新地提出了求解非線性方程組的方法,根據拉索-阻尼器系統的阻尼特性,在各種荷載作用下,對索-阻尼器系統的非線性瞬態振動響應進行了研究,從系統響應的角度更加直接地了阻尼器的控制效果。
  17. Yeast one - hybrid assay revealed that all of the four dreb proteins specifically bound to the dre element

    經酵母單,每個dreb蛋白都能與順式作用元件dre結合,啟動下游基因的表達。
  18. Pit13, an interactor binding to trpt1 protein, was screened by yeast two - hybrid, and confirmed by pull down and co - ip assays. the association of trpt1 with pit 13 demonstrated that trpt1 should probably participate in other activities besides in pre - trna splicing. more experiments will be required to determine the role of pit 13 protein

    0酵母以篩選、加衛工加wn檢測及ip實明m卜1 」且與功能未知的pit13蛋; 」之間存在著相互作用,說明imj基因除了參與trna剪接之外,還可能具有其它方面的功能,有待進一步研究。
  19. Metallothionein - 3, previously named as growth inhibitory factor, is the unique member of the metallothionein family that demonstrates bioactivity in vitro. to test if mt - 3 dimerizes or polymerizes itself

    為了其在細胞內是否形成二聚或多聚體,構建酵母雙系統用於檢測。
  20. Sds - page showed efficient expression and secretion of the rhfl. the highest yield ( 108 mg / l ) was obtained when expression was induced with 0. 5 % methanol for 96 hours

    Western出現了可被抗體特異性識別的帶,實此條帶即為重組畢赤氏酵母km7lppicgkil分泌表達的重組fl蛋白。
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