鼠得克 的英文怎麼說
中文拼音 [shǔdekè]
鼠得克
英文
difenacoum-
Amongst us a simpleton, possessed by the demon of hate or cupidity, who has an enemy to destroy, or some near relation to dispose of, goes straight to the grocer s or druggist s, gives a false name, which leads more easily to his detection than his real one, and under the pretext that the rats prevent him from sleeping, purchases five or six grammes of arsenic - if he is really a cunning fellow, he goes to five or six different druggists or grocers, and thereby becomes only five or six times more easily traced ; - then, when he has acquired his specific, he administers duly to his enemy, or near kinsman, a dose of arsenic which would make a mammoth or mastodon burst, and which, without rhyme or reason, makes his victim utter groans which alarm the entire neighborhood
在我們的人社會里,一個傻瓜要是心裏懷有仇恨或動了貪念,想除掉一個仇人或除去一個近親,他就會徑自跑到雜貨店或藥房裡,借口老鼠吵得他無法睡覺,要買五六克砒霜,他還會捏造一個假名字,而那卻比真名字更容易被識破,假如他真是一個狡猾的傢伙,他就會分別到五六家不同的藥房或雜貨店裡去買,因此,當追蹤線索的時候,就更容易了五六倍。然後,當他弄到他想要的東西以後,他就莽莽撞撞地給他的仇人或近親吃一付砒霜,其份量之重,就是古代的巨象或恐吃了也會五臟崩裂的,就這樣毫無意義地使他的受害者在那裡呻吟,以致驚動了四鄰。2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage
Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料,經傳代培養,獲得能連續而穩定傳至6代的,具有胚胎幹細胞諸多特性的eg細胞系。She must find mr. craddock precious dull if she prefers blackbeetles and stuffed kangaroos.
如果她寧願去看蟑螂和脹飽了的袋鼠,她一定是覺得克雷杜克先生呆頭呆腦。The whole defence made mistakes today, not just kalac. just because he is from australia, it does n ' t mean that he can jump over the bar like a kangaroo ? ac perugia coach serse cosmi defends goalkeeper zeljko kalac from the critics after one heavy defeat
他來自澳大利亞,但這並不意味著他能像袋鼠那樣跳得比橫梁還要高-佩魯賈隊教練瑟斯-科斯米在慘敗后為備受指責的守門員澤爾科-卡拉克辯護。The whole defence made mistakes today, not just kalac. just because he is from australia, it doesn ' t mean that he can jump over the bar like a kangaroo ? ac perugia coach serse cosmi defends goalkeeper zeljko kalac from the critics after one heavy defeat
不光是卡拉克,今天整條後防線都在犯錯。他來自澳大利亞,但這並不意味著他能像袋鼠那樣跳得比橫梁還要高- -佩魯賈隊教練瑟斯-科斯米在慘敗后為備受指責的守門員澤爾科-卡拉克辯護。Anti - melatonin monoclonal antibodies of higher titer, affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections. we obtained 6 strains of hybridoma, all of them secreting specific antibodies to mt, we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique. an inhibition curve for mt was obtained in the range of 50pg to30ng, and 1. 4ng of mt inhibited the value of the assay by half. we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues, the moabs recognized mt but
通過將mt用甲醛作連結劑連結到牛血清白蛋白上sa採用皮下多點注射兔疫小鼠得到了高效價,高親和力,較好特異性的抗mt單克隆抗體,最後獲得了5株單克隆細胞株,都能分泌針對mt的特異性抗體,建立了選擇性基團免疫分析法,用制備的抗體測定了人血清中mt的含量,作了mt的抑制標準曲線,其抑制范圍從50pg ? 30ng ,半抑制量為1Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line
本研究分為三個部分,其主要實驗方法及實驗結果如下:第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段( be644542 )入手,設計了基因特異性引物和載體特異性引物進行巢式pcr擴增,結合人類基因組草圖搜索法,從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna , genbank登錄號為ay040204 ,同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因, genbank登錄號為af395083 。Ptxb1 - hng and ptxb1 - m - insulin are expressed in e. coli successfully. after sds - page and densitometric scan analysis, the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )
Pbv220 ? hng在大腸桿菌中未檢測到表達,后兩個克隆在大腸桿菌bl21 ( de3 )中獲得高效表達, hng及m - insulin融合蛋白表達量分別佔全菌蛋白的40及50左右;經western - blot鑒定m - insulin融合蛋白可以與小鼠抗人胰島素單克隆抗體( igg )發生抗原抗體結合反應。The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鑒定、純化重組質粒后,脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞( cho - dhfr ~ - )中, g418選擇轉染子並克隆化培養,經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄,獲得4株穩定表達54位密碼突變型mbl的cho細胞。Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months. the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa. through three clonings less diversed anti - p24 hybridoma cells are gained
方法:基因工程p24抗原免疫小鼠4次,歷時2個月,取脾細胞與骨髓瘤細胞株sp2 0 ,用peg融合, hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞,三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。Positive single clonal cells were selected with g418 and pcr. after proliferating, the transfected cells immobilized and cultured in soldium alginate were induced by hormone
利用脂質體包裹重組質粒plncxhlf ,導入到小鼠乳腺癌細胞株ma - 782中, g418及pcr篩選獲得陽性單克隆細胞。You give away horses worth a thousand louis ; you save the lives of ladies of high rank and beauty ; under the name of major brack you run thoroughbreds ridden by tiny urchins not larger than marmots ; then, when you have carried off the golden trophy of victory, instead of setting any value on it, you give it to the first handsome woman you think of !
您拿價值一千路易的馬來送人您救了一位既有地位又漂亮的太太的性命您以布萊克參謀先生的名義去參加賽馬,派去了純種的駿馬和並不比土撥鼠大多少的騎師當您奪得了獎杯以後,卻毫不珍惜它,把它送給了您所想得到的第一個漂亮女人。 」As far as the first category is concerned, there are only very few mentions of what they eat, obviously because they don ' t have different appetites in the potter universe : sirius lives on rats while he is hiding as a dog outside hogsmeade ( gf ), crookshanks craves spiders and rats ( well, wormtail, but anyway ) and owls go hunting ( only little pigwidgeon gets an owl treat from time to time )
至於普通的生物吃什麼是很少在書中提及的,很明顯是因為它們的飲食習慣即使在哈利的世界中也和普通的世界沒什麼不同:小天狼星變成狗躲在霍格莫德村時靠老鼠過活;克魯克山最喜歡蜘蛛和老鼠(哦,無論如何蟲尾巴也是一隻老鼠啊) ,而貓頭鷹則外出捕獵(只有小豬能經常得到貓頭鷹應有的待遇) 。Cloning techniques have continued to improve since then, and today there are cloned mice, salmon, and cattle
克隆技術從那時起得到了持續的進展,今天已有了克隆老鼠、克隆鮭魚和克隆牛。The mass production of carbon copy mice, in turn, now allows researchers to test variations of the cloning techniques to see what works best
而老鼠的大規模復制反過來也使得研究人員能夠測試各種不同的克隆技術,確定哪能一種最佳。Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography
方法: pcr擴增小鼠pemcdna編碼序列,將它克隆到含有gst的原核表達質粒載體pgex - 4t - 3上,轉化大腸桿菌bl21 ,誘導表達gst mpem融合蛋白,通過親和層析,獲得初步純化的產物,以羥胺切割驗證其一級結構。The main contents and results were as follows : 1. the establishment of sd rat fibroblast for the donor of nuclear transfer the method of single cell cloning by micro - manipulating was improved to purify fibroblast. as a result, steady fibroblast can be obtained and the rate of the clone formation is 92. 71 %
主要內容和結果如下: (一) sd大鼠成纖維細胞系的建立將單細胞顯微法改進為單細胞集落克隆純化,能快速、有效建立細胞系,並可獲得92 . 71克隆率。The components of 37kd and 39kd glycoproteins from gel were immunized respectively mice and polyclonal antiserums were obtained. glycoproteins of sperm cell surface can be examined using igg rabbit anti mouse - colloidal gold as probe
用它們分別免疫小鼠得到多克隆抗體,以兔抗鼠igg膠體金15nm為探針,檢查此兩種蛋白在精細胞質膜上的分佈。分享友人