amplification reaction 中文意思是什麼

amplification reaction 解釋
放大反應
  • amplification : n. 1. 擴大；擴充。2. 【電學】增幅，放大（率）。3. （聲明等的）補充材料。
  • reaction : n 1 反作用，反應；反沖；反動力。2 【政治學】反動，倒退；復古（運動）。3 【化學】反應，【物理學】...
  1. Asymmetric autocatalysis intrinsically has the following advantages : chiral amplification of production, high reaction efficiency, easy purification of the products, consecutive reaction, which is a new aspect of chiral synthesis

    不對稱自催化具有手性自動放大、反應活性較高、產物處理較易、反應體系連續等特點，是不對稱化學的一個新的領域。
  2. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  3. Dig labeled probe hybridization with solid pcr product was performed as well as electrophoresis of liquid product, this method combines rna purification, pcr amplification and nucleotide probe hybridization detection together and has many advantages including better rna purity, less time consumption, reliable positive reaction and 10 times sensitivity as rt - pcr gel running detection, reduce false positive result, unpurified nucleotide requirement, loug infected plant organism can be detected by solid hybridization

    2 ）結果可靠，雜交特異性誘捕目的片段，同時去除了核酸中存在的pcr抑制物質，減少了因核酸提取不純造成的漏檢現象，結果輸出通過雙重判定保證了結果的可靠性。 3 ）靈敏度高，通過雜交進行結果判定，靈敏度比傳統的rt干cr大約高10倍。
  4. The ghr cdna was isolated from southern catfish ( silurus meridionalis ) by reverse transcription polymerase chain reaction followed by rapid amplification of cdna ends

    用rt - per加race的方法從南方大口鯰中克隆出其生長激素受體（ ghr ） 。
  5. To isolate hfl -, hf2 gene encoding flavonoid - 3 ', 5 ' - hydroxylase ( f3 ', 5 ' h ) from petunia hybrida of the purple flower, we used a polymerase chain reaction ( pcr ) amplification using the genomic dna as a template. the result indicated that the hfl dna was 2797bp in total length and encoded 506 amino - acid, containing three extron and two intron ; hf2 dna was 2223bp in total length and encoded 509 amino - acid, containing three extron and two intron. comparison of the sequence with genbank databases revealed that hfl dna and hf2 dna shared significant nucleotides identity ( 98. 97 % and 99. 04 % respectively ) and amino - acid identity ( 99. 41 % and 99. 41 % respectively )

    Hf1dna全長2797bp ，包括3個外顯子， 2個內含子，其中一個開放閱讀框架（ orf ）編碼506個氨基酸，與文獻報道的序列相比較，具有98 . 97核苷酸同源性與99 . 41氨基酸同源性： hf2dna全長2223bp ，包括3個外顯子， 2個內含子，其中一個開放閱讀框架（ orf ）編碼509個氨基酸，與文獻報道的核苷酸同源率為99 . 04以及氨基酸同源率為99 . 41 。
  6. Polymerase chain reaction is a rapidly developing and widely used dna amplification technique, which is widely applied in life science and other related fields

    聚合酶鏈式反應（ polymerasechainreaction ，簡稱pcr ）技術是發展很快、應用很廣的體外擴增基因片斷技術，在生命科學研究及諸多相關領域已經得到了廣泛應用。
  7. The work mainly consists of four parts : the first part is to use oxidation and lpcvd technique to produce sio2 mask film and si3n4 insulation film in order to enhance the heating efficiency of micro chamber, and guarantee the carry out of the reaction. the second part is to use the combination of dry etching and wet etching to produce reaction micro chamber, it is the container which carry out the pcr reaction, and dna sample carry out amplification reaction here. the third part is to use the sputtering, photolithography to produce heaters and temperature sensors which heat the reaction micro chamber and provide the temperature condition for the pcr reaction

    首先，利用氧化工藝和lpcvd技術，生長sio _ 2掩膜層和si _ 3n _ 4絕緣層，以提高反應腔的熱效率，保證擴增反應的順利進行；其次，用濕法腐蝕和干法刻蝕相結合的方法加工微型腔體，使之作為dna樣品進行pcr擴增反應的容器；第三，用濺射、光刻等工藝在微型腔體底部製作微型加熱器和溫度傳感器，實現對反應腔體的加熱及其溫度的精確測量，提供pcr擴增反應所需的溫度條件。
  8. The fourth part is temperature control system which ensure the pcr reaction performing accurately. after analysis of the experiment result and optimization of technics, we finally come up with relatively ideal technics situation and produce relatively ideal micro chamber, ni temperature sensors and ni / cr heaters, which can provide and test the temperature condition for pcr amplification

    經過不斷地分析實驗結果，改進製作方法，最終探索出了較好的工藝條件，制備出了較理想的微型腔體、鎳薄膜電阻溫度傳感器和鎳鉻合金薄膜電阻加熱器，能提供和檢測pcr擴增反應所需的溫度，並實現了三溫區的循環控制。
  9. A novel real - time fluorescent rt - pcr was established to detect pnrsv this technique uses a taqman probe to monitor in real time amplification of target genes, a taqman probe labeled by a quenching and fluorecenting was added to the pcr reaction buffer

    另外還設計了taqman熒光探針，確定了檢測條件和參數，建立了實時熒光rtwcr方法，該方法是指在pcr反應體系中加入帶有熒光基團互補探針，如果有pcr反應（擴增） ，熒光信號就較大。
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