competitive pcr 中文意思是什麼
competitive pcr
解釋
競爭（性）- competitive : adj 競爭的，競賽的。 competitive bidding system 招標制。 competitive examination 競爭考試。 compe...
- pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
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Genetic diversity and phylogeny of 55 slow - growing rhizobia isolated from peanut ( arachis hypogaea ) in china were determined by analysis of host - plant range, phynotype, 16s rrna rflp, 16s rrna sequence, 16s - 23s igs rflp, rapd, rep - pcr, dna - dna hybridization homology. at the same time, the competitive nodulation capacity of rhizobia, effect of host plants and soil ph on the rhizobia were determined for screening and improvement of high effective rhizobium inoculant
本研究採用宿主范圍試驗、表型性狀測定、 16srrna - rflp 、 16srrna序列分析、 16s - 23srdnaigsrflp分析、 rapd分析、 rep - pcr分析和dna - dna同源性分析等技術系統研究了從我國不同地域分離的55株花生根瘤菌的遺傳多樣性及其在根瘤菌系統發育中的地位和相互關系。 -
Improvement of competitive rt - pcr in quantification of per1 gene expression
1基因表達中的應用 -
This project is aimed at : ( 1 ) expressing melittin and megf in e. coli respectively ; ( 2 ) constructing a membrane - toxic immunotoxin by using melittin as toxic part and megf as target part ; ( 3 ) measuring the megf mrna expression abundance by quantitative competitive rt - pcr ; ( 4 ) checking the genetic stability of the recombinant strains constructed in the project ; ( 5 ) optimizing the fermentation conditions of the target strains by flask shake
本課題以大腸桿菌（ e . coli ）宿主重組表達melittin和megf ；以melittin為毒性部分、 megf為靶向部分，構建膜毒性免疫毒素；用定量競爭rt - pcr法測定megf體內表達豐度；檢驗所構建的各重組菌株的遺傳穩定性；採用搖瓶分析對各重組菌株的發酵條件進行了初步優化。 -
To quantitatively measure the each cytokine levels from the spleens obtained before or after immunized mice, the total rna were extracted and generated into cdna by rt reaction. the native cdna was subjected into a competitive pcr reaction with a competitor that is a slightly larger in size
為了對免疫前後小鼠脾臟表達的細胞因子的水平進行定量檢測，提取小鼠脾臟組織總rna ，經rt反應得到cdna ，利用一種修飾的競爭模板進行競爭pcr擴增，在瓊脂糖凝膠電泳分析並與競爭模板產物比較而定量。
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