dna probe analysis 中文意思是什麼

dna probe analysis 解釋
dna探查分析
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • probe : n 1 【醫學】探針；探示器；取樣器；【物理學】試探電極。2 【醫學】（對傷處等的）針探，探查；刺探；...
  • analysis : n. （pl. -ses ）1. 分解，分析；【數學】解析。2. 梗概，要略。3. 〈美國〉用精神分析法治療（＝ psychoanalysis）。
  1. We found that sit variant gene ( slt2vha ) was identified in strains e. coli o157 : h7 isolated from patients and dung beetles 2000 in xuzhou city, jiangsu province. the primers used for stx2 variant analysis are shown in tablel. genomic dna restriction fragments digested by pstiwere sonthern - blotted and hybridized with an stx2 - specific dna probe. the probe was prepared fromed a 285 - bp pcr productof the strain 882364 stx2 gene obtained by using the specific primer pair ( tablel )

    2000年在江蘇省徐州市銅山縣腹瀉病患者的糞便標本分離的10株產生志賀毒素的菌株以及從蜣螂腸道分離到的4株產生志賀毒素的大腸桿菌o157 ： h7 ，屬于另兩個pfge型，和1986 、 1987 、 1988年在徐州市腹瀉病患者的糞便標本分離的菌株pfge圖譜不同。
  2. How to obtain the useful biochdrical informaton on this scale is the new tren in the research fie1d of analytical chehascy therefore, single molecule detection, sing1e cell detection, dna ~ and the shaple dna analysis were one of the main research direeons ofanalytcal chendscy nove1 molecular probe and ultrasmali biosensor for real tiine and in vivo detection has been the focuses in the research field of analytical chendstry according to the above mentioned advanced direetions, two pnd of inveshgations has been pdrirmed in thes thesis

    人們對生命現象的觀察和研究已經深入到納米尺度和單細胞，單分子的水平，如何在這樣一個尺度范圍內獲取有用的生物化學信息對分析化學的各個研究領域均提出了新的要求。單分子、單細胞檢測、生物晶元的開發以及納米技術的應用漸漸成為現代分析化學研究的主流領域之一。可進行實時、在線、原位、活體檢測的分子探針和超微型生物傳感器成為人們研究的熱點和重點。
  3. Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule

    其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。
  4. A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli. endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern. the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18. the ligation mixture was used to transform e. coli jm109and the transformants were plated on lb agar containing antibiotics. plasmid dna containing cloned genes were used for direct sequencing

    提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產物的hae 、 ras酶切分析，以及pcr產物的序列分析，發現2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 ： h7菌株僅僅攜帶slt2vha基因。
  5. Ih ped one, a nove1 mo1ecuar probe onolecuar beason ( mb ) was developed to establish a stwle and precise method for the quanitative analysis of gentic materials such as ing1 inrn from differen cells and rna fonn tobacco mosaic virus. the milization method for mb was studied for the fabrication of dna biosensor and dna chip. ih pall two, a series of inunobilization method such as sol - gel method and ligh polererization method were investigaed for the otilization of bio - molecues and fluorescence dye

    本論文各章內容可歸納如下：第一章，根據抑癌基因ing1基因的序列設計併合成了檢測ing1轉錄產物的分子信標（ 5 ' - - tamra - c6 - nh - cgttgatggttccacttctcgtgcgttcaacg - dabcyl - 3 ' ） ，其中tamra代表四甲基羅丹明，為熒光基團， dabcyl代表4 - （對二甲氨基偶氮苯）苯甲酸，為熒光熄滅基團。
  6. The distribution and amount analysis of these bacteria in different layers of core sediment indicated that there was an intact cycle that coupled sulfur metabolism with methane metabolism existed in this area, which may be the microbial response to the environment because there was seldom similar bacteria detected from " manganese nodule " area sediment by dna - dna hybridization with specific oligonucleotide probe and 16s rdna clone library analysis

    而16srdna克隆文庫分析和dna - dna雜交的結果表明「結核」區沉積物中這兩類細菌數目很少，說明「暖池」區沉積物中的微生物群落結構特徵是對環境因素的一種響應，同時也可能是影響該海區深海及海洋環境的一個重要因素。
  7. The quantitative determination of protein and dna is a common analysis contents in biology chemistry and life science. so it is very important to find fluorescence probe with high sensitivity and low detection limit

    對蛋白質和dna進行定量測定是生物化學和生命科學中經常涉及的分析內容，建立高靈敏度、低檢測限的熒光探針定量測定的方法仍具有重要意義。
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