dna probe 中文意思是什麼
dna probe
解釋
dna探針-
Mouse ; restriction fragment length polymorphism ; dna fingerprint ; probe jl - 02 ; es cell
小鼠限制性片段長度多態性dna指紋圖jl - 02探針胚胎幹細胞 -
Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers
嘗試應用多位點dna指紋技術，檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明：採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜，具有足夠的多態性和很好的穩定性。 -
Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city
最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。 -
Along with the development of the cytobiology and the molecular biology, and thoroughly research of the biophysics, the biochemistry, the genetics and immunology, it has cultivated the modem biological technology, such al genetic engineering, cellular engineering, enzyme engineering, fermentation engineering and so on, to change biology characteristic to carry on the material transformation, has formed the front biological examination technology : the dna probe, the pcr technology, the molecular mark, the bioluminescence technology, genechip technology and so on the widespread application of these advanced biotechnologies in dairy industry baa impelled the dairying technical transformation, and has been having vital significance to dairy production, research and dairy product security
摘要隨著細胞生物學和分子生物學的發展及對生物物理、生物化學、遺傳學和免疫學研究的深入，培育了基因工程、細胞工程、酶工程、發酵工程等改變生物特性進行物質轉化的現代生物技術，形成了dna探針、 pcr技術、分子標記、生物熒光技術、基因晶元技術等前沿性的生物檢測技術，其在乳品工業中的廣泛應用，推動了乳業的技術變革，對乳品生產、研究和乳品安全意義重大。 -
The signal provided by the probe is a measure of the degree of hybridization. traditional dna probes are labeled with radioisotopes i. e. 32p, i25i, 3h or 35s
傳統的dna分子雜交採用的是放射性標記的檢測方法，這種方法雖然靈敏度高，但存在放射性物質對人體及環境的危害。 -
The love wave device with the substrate of st - cut quartz and the guiding layer of si02 was used as the saw biosensor. dna sensor employing synthetic seb dna probe as a molecular recognizing component was utilized to detect seb by measuring seb dna in samples
該傳感器採用以st一切割的石英晶片為基體，以510 :為波傳導層的樂甫波（ lovewaves ）裝置，利用具有分子識別功能的dna合成探針來實現對樣品中sebdna的檢測。 -
We found that sit variant gene ( slt2vha ) was identified in strains e. coli o157 : h7 isolated from patients and dung beetles 2000 in xuzhou city, jiangsu province. the primers used for stx2 variant analysis are shown in tablel. genomic dna restriction fragments digested by pstiwere sonthern - blotted and hybridized with an stx2 - specific dna probe. the probe was prepared fromed a 285 - bp pcr productof the strain 882364 stx2 gene obtained by using the specific primer pair ( tablel )
2000年在江蘇省徐州市銅山縣腹瀉病患者的糞便標本分離的10株產生志賀毒素的菌株以及從蜣螂腸道分離到的4株產生志賀毒素的大腸桿菌o157 ： h7 ，屬于另兩個pfge型，和1986 、 1987 、 1988年在徐州市腹瀉病患者的糞便標本分離的菌株pfge圖譜不同。 -
Biotin - avitin system ( bas ) was used to immobilize dna on the pdms channel compared to the direct immobilization methds. the hybridization reaction also has been examined between immobilized probe and target dna
針對pdms新型材料，試驗了兩種dna分子的固定方法，確定了生物素?親和素介導的dna分子固定化方法，並使用此晶元和固定方法進行了晶元上的dna雜交反應。 -
How to obtain the useful biochdrical informaton on this scale is the new tren in the research fie1d of analytical chehascy therefore, single molecule detection, sing1e cell detection, dna ~ and the shaple dna analysis were one of the main research direeons ofanalytcal chendscy nove1 molecular probe and ultrasmali biosensor for real tiine and in vivo detection has been the focuses in the research field of analytical chendstry according to the above mentioned advanced direetions, two pnd of inveshgations has been pdrirmed in thes thesis
人們對生命現象的觀察和研究已經深入到納米尺度和單細胞，單分子的水平，如何在這樣一個尺度范圍內獲取有用的生物化學信息對分析化學的各個研究領域均提出了新的要求。單分子、單細胞檢測、生物晶元的開發以及納米技術的應用漸漸成為現代分析化學研究的主流領域之一。可進行實時、在線、原位、活體檢測的分子探針和超微型生物傳感器成為人們研究的熱點和重點。 -
This is the first time scientists hae been able to probe the " dark " energy state - - so called because it cannot be detected by fluorescence techniques used to study other high - energy states created in dna by u light
這是科學家首次能夠檢測到「黑暗狀態」 ，命名緣于熒光技術下（通常用於檢測紫外光在dna產生其他高能狀態）無法檢測其存在。 -
This is the first time scientists have been able to probe the " dark " energy state - - so called because it cannot be detected by fluorescence techniques used to study other high - energy states created in dna by uv light
這是科學家首次能夠檢測到「黑暗狀態」 ，命名緣于熒光技術下（通常用於檢測紫外光在dna產生其他高能狀態）無法檢測其存在。 -
Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence
以pcr擴增的人血清白蛋白（ hsa ）基因片段為探針，從人的基因組文庫中雜交篩選的陽性克隆中，經測序分析，有一個克隆含有全長hsadna ，用從其它的陽性克隆中選取兩種dna片段，即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ，與人hsadna一起，進行顯微共注射，成功制備了轉多基因小鼠。 -
Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule
其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。 -
A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli. endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern. the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18. the ligation mixture was used to transform e. coli jm109and the transformants were plated on lb agar containing antibiotics. plasmid dna containing cloned genes were used for direct sequencing
提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產物的hae 、 ras酶切分析，以及pcr產物的序列分析，發現2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 ： h7菌株僅僅攜帶slt2vha基因。 -
The genomic dna extracted from three rubber tree strains ( pr107, rrim600, gt1 ) was digested with hinckr. the southern blot was made with the same probe as above. the result showed that all the three strains turned up only one brand ( - - 4. 8kb ). the three strains had the same inherent background
用橡膠樹pr107 、 rrim600 、 gti三個品系的基因組洲a分別經hinan酶切后，進行了southern雜交。結果表明，三個無性系都只出現一條雜交帶，位置一』致（ 4 -
The specificity of the nucleic acid probe was very strict. lt reacted positively with iltv dna only and it react negatively with the nuleic acid of ndv, bv and ibdv. the sensitivity of this kind of probe is very high. ilt could even detect 20pg ' s iltv dna
結果表明：該種核酸探針具有高度的特異性，它僅與iltv的dna呈現陽性反應，而與新城疫病毒、傳染性法氏囊病病毒和傳染性支氣管炎病毒的核酸等均呈陰性反應。該種探針具有高度的敏感性，能夠檢測到20pg的iltv的dna 。 -
4. by using absorption spectra method and eb as a fluorescence probe, we studied the action modes of the complexes binding to dna
用紫外吸收光譜法、熒光光譜法（ eb為探針）詳細研究了鎳、錳、鋅與dna的作用方式。 -
The dna electrochemical sensor was prepared by self - assembling the designed hepatitis b virus dna specific probe on the surface of au electrode together with hoechst 33258 as electro - active indicator
利用自組裝單分子膜法，將人工合成的乙肝病毒單鏈dna片斷作為特異性探針固定在金電極表面，結合電活性指示劑hoechst33258構成dna電化學傳感器。 -
In this thesis, the energy transfer system of acridine orange ( ao ) - rhodamine b ( rb ) dimer was built for the determination of dna as fluorescence probe
本論文以吖啶橙?羅丹明b二聚體能量轉移體系作為熒光探針，建立了測定dna的新方法。 -
Ih ped one, a nove1 mo1ecuar probe onolecuar beason ( mb ) was developed to establish a stwle and precise method for the quanitative analysis of gentic materials such as ing1 inrn from differen cells and rna fonn tobacco mosaic virus. the milization method for mb was studied for the fabrication of dna biosensor and dna chip. ih pall two, a series of inunobilization method such as sol - gel method and ligh polererization method were investigaed for the otilization of bio - molecues and fluorescence dye
本論文各章內容可歸納如下：第一章，根據抑癌基因ing1基因的序列設計併合成了檢測ing1轉錄產物的分子信標（ 5 ' - - tamra - c6 - nh - cgttgatggttccacttctcgtgcgttcaacg - dabcyl - 3 ' ） ，其中tamra代表四甲基羅丹明，為熒光基團， dabcyl代表4 - （對二甲氨基偶氮苯）苯甲酸，為熒光熄滅基團。
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