dna protein 中文意思是什麼

dna protein 解釋
脫氧核糖核酸蛋白
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • protein : n. 【化學】朊，蛋白（質）。
  1. Even so, by truncating hbv pres gene, we finally obtained some useful " " bailors ", either nontoxic or self - activating, and used them to fish dna fragments of hbv pres interacting protein ( s ) from an ad vector constructed human embryonic cdna library

    我們通過第回軍巨大學碩士學位論文對pres基因分段截短的方法，獲得了對酵母細胞即無毒性作用，又沒有自激活作用的「誘餌」 ，通過它在酵母雙雜交系統中篩選構建於ad載體的人胎肝。
  2. It ' s meaningful to study the function of rab proteins in ciliates and further to explicit the mechanism of vesicular trafficking. the rob gene was amplified from macronuclear dna of euplotes octocarinatus. the size of gene was 783 bp long with an orf of 624 bp encoding eorabl protein and containing three in - frame tga codes

    本研究利用pcr技術從游仆蟲（ euplotesoctocarinatus ）大核dna中擴增出rab基因，並對該基因進行序列分析，該基因全長為783bp ，兩端為端粒序列，編碼框為624bp ，編碼207個氨基酸，開放讀框中有3個tga ，在此編碼半胱氨酸。
  3. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. Molecular genetics came to depend upon studies of the structure and function of dna, rna and protein.

    分子遺傳學的形成仰賴于DNA，RNA和蛋白質的結構與功能的研究成果。
  6. 2 the polymorphism of ul136 gene dna and amino acid sequence 1 ) the length of ul136 orf in all 18 clinical isolates was similar to that of toledo, 723 bp in size. they hade the potential to encode 241 amino acid protein

    ? 2 ?二月136基因編碼區及編碼產物氨基酸序列的多態性株臨床低傳代分離株ul136orf長度均與toedo株相同，為723hp ，預測編碼241個氨基酸的蛋白。
  7. Sedegah m, hedstrom rc, hobart p, et al. protection against malaria by immunization with plasmid dna encoding circumsporozoite protein [ j ]. proc natl acad sci usa, 1994, 91 ( 21 ) : 9866

    劉彥文，余新炳，徐勁等.惡性瘧原蟲海南株環子孢子蛋白基因的克隆與表達[ j ] .中國人獸共患病雜志， 2000 ， 16 （ 1 ） : 8
  8. The dna sequence had a complete orf ( open reading frame ), which coded a protein of 479 amino acid residues. the protein sequence of enolase which contained the conserved domain, was homologue to enolase of other organisms. it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii

    並將其中的一個gus基因用目的片段烯醇酶基因替換，構建了可以在植物中高效表達的載體pcambia2301g一enolase ，成功地將其轉入根癌農桿菌eha105中，為下一步進行轉基因植物的研究作準備。
  9. Tsarg2 with 1 233 bp length was composed of 6 exons and spaned about 115 kb of genomic dna, the putative protein encoded by this gene was 305 amino acid with a theoretical mass of 34 751 and with no significant homology with any known protein in databases. a kind of nucleoprotein was the most impossible

    Tsarg2基因的cdna全長為1233bp ，包含6個外顯子，基因組跨越115kb ，編碼由305個氨基酸組成的、分子量為34751的蛋白質，與已知蛋白質無明顯同源性，其最大可能是一種核蛋白。
  10. We immunize the balb / c mice with pc4. 0f and pc3. 1f by paunch and muscle administration and immunize each with different content of 10 g, 50 g 100 g. the antibodies in serum against newscastle disease virus f gene protein are tested by elisa. the result showes that two kinds of dna vaccines can induce specific antibody response to encoded proteins in 15 weeks by im and ip, but antibodies response is little different

    免疫結果顯示，採用2種免疫途徑（肌肉注射，腹腔注射） ， 3個劑量（ 10 g 、 50 g和100 g ）免疫balb c小鼠，在被檢測的15周內，肌肉注射和腹腔注射組均可產生抗體，抗體的產生有明顯的劑量依賴性， 50 g只和100 g只明顯優於10 g只，而100 g只與50 g只相似（肌肉注射）甚至稍低（腹腔注射） 。
  11. Sequencers and synthesizers for dna and protein

    及蛋白質的測序和合成儀
  12. A virion consists of a protein coat surrounding one or more strands of dna or rna

    一個病毒粒子包括外部的蛋白衣殼和內部的一條至多條dna或rna鏈。
  13. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  14. There were some degration in the purified protein, but gst - ap - 2 a still had the dna binding activity in the gel shift assay. the gst - testin and gst - antn1 were used for immunolize rabbits

    雖然所提取的融合蛋白gst - ap - 2出現較多降解，但是電泳遷移率變動分析顯示其仍然具有良好的dna結合活性。
  15. The insulin works by switching on cellular signaling proteins called kinases ( specifically src, pi3k, and akt ) and a protein ( srebp ) that binds elements in dna that regulate the production of cholesterol and its relaties

    胰島素功能在於激活一類叫作激酶的細胞信號蛋白（特定的致敏反應細胞，如磷脂酰肌醇（ - 3 ）激酶，蛋白激酶b等）以及一種dna元素結合蛋白（膽固醇調節元件結合蛋白） ，這種蛋白質能調節膽固醇及類膽固醇的生成。
  16. The insulin works by switching on cellular signaling proteins called kinases ( specifically src, pi3k, and akt ) and a protein ( srebp ) that binds elements in dna that regulate the production of cholesterol and its relatives

    胰島素功能在於激活一類叫作激酶的細胞信號蛋白（特定的致敏反應細胞，如磷脂酰肌醇（ - 3 ）激酶，蛋白激酶b等）以及一種dna元素結合蛋白（膽固醇調節元件結合蛋白） ，這種蛋白質能調節膽固醇及類膽固醇的生成。
  17. Phage display describes a selection technique in which a peptide or protein is expressed as a fussion with a coat protein of bacteriophage, resulting in display of the fused protein on the surface of the virion, while the dna encoding the fussion resides within the virion

    自1985年gpsmith首次提出噬菌體展示技術以來，隨著生物技術的發展，噬菌體隨機肽庫已成為研究分子間相互作用的有力工具，特別是在抗原表位研究方面。
  18. As we enter your rna / dna protein strands, carefully observe the fabulous light show

    如同我們進入你的rna / dna蛋白質條內那樣，仔細觀察這神話般的光顯示。
  19. Investigating the interaction mechanism of metal or metal complex with biomolecule, such as dna, protein and amino acid, provides a starting point for the diagnosis of metal - induced human disease

    金屬及其配合物與dna 、蛋白質、氨基酸等生物分子的作用機理研究，是診斷和治療由金屬引起的疾病的重要環節。
  20. Time - order effects of vitamin c on hexavalent chromium - induced dna - protein crosslinks in rat peripheral blood lymphocytes

    姜黃素對順鉑所致大鼠腎毒性的防護作用
分享友人
分享到 Line

分享到臉書