dna sequence analysis 中文意思是什麼

dna sequence analysis 解釋
dna序列分析
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  • analysis : n. （pl. -ses ）1. 分解，分析；【數學】解析。2. 梗概，要略。3. 〈美國〉用精神分析法治療（＝ psychoanalysis）。
  1. Dna sequence analysis indicated that tn5gusa5 is prone to insert into low gc content regions ; guanine is a preferential base at the first place and cytosine at the last site of target sequence

    在質粒上tn5gusa5也傾向于插入低gc含量區；堿基g和c分別在靶序列的首位和末尾出現的幾率高。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Hemagglutinins inhibition ( hi ) testes showed that there were obvious variations among the isolates from different geographical areas. this result explained why the protection rates of current vaccine were different around the country. dna sequence analysis of h9 hemagglutinin genes showed that these viruses were closely related and possibly came from one source

    且在同一地域內，不同的異變體之間的差異較小，隨著時間的推移，其變異並不明顯；但在不同的區域內分離的毒株之間變異較為突出，這表明中國大陸h9n2亞型禽流感病毒不同分離株抗原性是與地域的改變而發生較為明顯的變異。
  4. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  5. Cloning and sequence analysis of nosema bombycis s special dna fragment

    片段的克隆與序列分析
  6. Due to these inherent advantages, ecl method has attracted much attention from all analytical fields, especially from biochemical analysis. in this dissertation we focused on the preparation of a new type of dna probes which were labeled with ecl activated substances. based on coupling with the dna hybridization and immobilization techniques, we have developed new ecl methods for the determination of special dna sequence

    本論文通過研究了多種ecl活性物質的發光性能，並以這些物質為標記物制備了多種高靈敏度的dna - ecl探針，結合dna雜交技術和dna固定化技術，將高靈敏度的ecl檢測手段應用於生命物質dna的序列識別及含量測定，為dna傳感器的研究和基因晶元的開發提供了新的思路和方法。
  7. Restriction fragment lenth polymorphism ( rflp ) analysis n random amplified polymorphic dna analysis [ rapd ], the sequence analysis of internal transcribed spacers ( its ) of ribosomal dna ( rdna ), induction of microcyclic conidiation, sem ( scanning electron microscopy ) ascosporal isolation and other methods were applied to study more than 100 specimens or isolates of cordyceps, its anamorphs and other entomogenous fungi

    本文採用了rapd 、 rdnaits的rflp 、 rdna的its序列分析、誘發微循環產孢、掃描電鏡觀察、子囊孢子分離等方法研究了蟲草及其他蟲生真菌的100多個標本或菌株。
  8. So must use information theory method depict and abundant the genetic diversity index system. in addition to, the introduce of molecule biology technology and the research of nucleotide sequence evolutive give a new method for population genetic, so must do deeply research about the analysis method of dna sequence data = the research main about the follows : there are three parts about the information model of population genetic : one about the shannon information entropy property of equilibrium population and the entropy change in the process of establish equilibrium ; another research is about the diversity measure - ment of genetic variation ; lastly, research the shannon information measurement about the disequilibrium gene variation. the result is : 1 to a definite gene distribution, the genotype entropy reach the maximum at the equilibrium population, the process of population from disequilibrium to equilibrium, the entropy get large and large

    此外，分子生物技術的介入及核苷酸序列進化的研究都為群體遺傳學的深入研究提供了新的途徑，但關于dna序列數據的分析方法需要作進一步的研究。本研究主要體現在以下幾個方面： （一）關于群體遺傳學的信息論模型研究，主要分為三部分內容：一是群體平衡的shannon信息熵的性質和群體平衡建立的熵變性質；二是群體遺傳多樣性測度的研究；三是非平衡群體的基因變異測量shannon信息量的方法研究。得到了如下結論： 1 、平衡群體的shannon信息熵最大，群體平衡的過程是熵的增大過程。
  9. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  10. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  11. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  12. The study indicates that mitochondrial dna sequence analysis is a useful tool to associate the larval and adult stages of trichoptera, in turn, it will be valuable for health assessment of stream ecosystems

    根據原雙棲紋石蛾成幼蟲的pcr反應電泳圖譜、序列同源性和酶切圖譜的一致性，基本確認了其成幼蟲聯系。
  13. An oligo - nucleotide primer was designed according to a conservative fragment of this gene of plants in genebank. two 3 ' - end coding sequences of this gene ( including complete sequences of exon 8, 7, 6, 5, 4 and partial sequences of exon 3 ), one, 1097bp, and the other 1109bp, were successfully isolated from brassicaeae napus and brassicaeae campestris. the dna sequence analysis shows that the two fragments are more than 90 % identical to orychophragmus violaceus, arabidopsis thaliana and brassicaceae napus

    同源對比分析已有的植物epsps基因序列，我們找到了該基因中一段極為保守的序列並設計了一條寡核苷酸引物，採用3 』 race法，從植物蜀雜9號油菜，白菜型油菜總rna中成功的擴增出了epsps基因3 』端編碼區（包含外顯子8 、 7 、 6 、 5 、 4的全部序列及外顯子3的部分序列） ，分別長1097bp ， 1109bp 。
  14. The obtained haplotypes are compared, aligned and analyzed by dna sequence analysis software package dnastar, dnasp, mega, to calculated residue composition, polymorphic sites and genetic distance, to consider the types and rates of residus substitution and to create neighbor - joining ( nj ) phylogenetic trees and determine phylogeny of the classification taxa involved in the research. 5

    5 、盲榕科昆蟲cytb基因序列分析方法和分析結果直接反映著被試分類單元的遺傳物質的差異和遺傳本質，是最為客觀的系統分類依據，所以可應用於盲峙科的種類的鑒別、種群的分析、種上類群的劃分以及科級階元水平系統發育關系的確定。
  15. Two dna fragments encoding d - hydantoinase gene were amplified by pcr from chromosome dna of pseudomonas putida yz - 26 and sinorhizobium morelense ss - ori, respectively, and confirmed by dna sequence analysis

    序列分析表明，來自yz - 26菌的d -海因酶基因全長1440bp ，編碼479氨基酸，基因序列已被genbank登錄（ ay387829 ） ，被確定為一個新基因。
  16. The 3. 4kb ecori dna fragment of the pgxn201 and the mutant ecori dna fragments of the three mutant plasmids were subcloned into the ecori sites of the clone vectors ml3 and the pgem3z - f ( + ) respectively. through dna sequencing and dna sequence analysis by comparing with the bradyrhizobium japonicum dna sequences reported, we found that there are four intact open reading frames in the 3. 4kb fragment and it was highly homologous to the bradyrhizobium japonicum dna sequence reported, and the tn5gusa5 containing in the pgxn217 was inserted in one of the four open reading frames. this open reading frame located on about 5. 5kb upstream of the nfec gene, which includes 207bp nucleotides, coding 68 putative amino acids and this is completely identical to that of the bradyrhizobium japonicum dna sequence reported

    本研究首先利用轉座子tn5gusa5對重組質粒pgxn201進行誘變，獲得3 . 4kbecori片段插入了tn5gusa5的突變質粒pgxn215 、 pgxn216 、 pgxn217 。對這些突變質粒進行亞克隆及測序分析並與基因庫中已報道的慢生型大豆根瘤菌的dna序列作比較，發現突變質粒pgxn217中tn5gusa5恰好插入在一個未知功能的開放閱讀框架內。將pgxn201的3 . 4kbecori片段與m13作連接，獲得亞克隆pgxn201c ，對pgxn201c進行測序分析，發現與基因庫中已報道的慢生型大豆根瘤菌的dna序列有95的同源性。
  17. Dna sequence analysis showed that the cloned nk gene was highly homologous to the sequence reported by nakamura. the deduced amino acid sequence also had the conserved sequences ( serine 221, histidine 64, and aspartic acid 32 ) which was essential for the catalytic center of serine proteases. the nk gene was then cloned into the transfer vector pvl1393

    本實驗以b . subtilis （ natto ）基因組dna為模板擴增了nk基因，測序結果顯示與nakamura報道的納豆激酶基因高度同源，其氨基酸序列也含有絲氨酸蛋白酶的活性保守中心（ serine221 ， histidine64 ， asparticacid32 ） 。
  18. Momp gene is amplified by using pcr technologyfrom dna of l2 trachoma chlamydia. the pcr products are recombined with vectors of pmd18 - t. more over, the recombinant plasmids are colonged. the dna sequence analysis shows the insert fragment is momp dna

    本實驗採用pcr技術，從l _ 2型沙眼衣原體dna中擴增出momp基因，與pmd18 - t載體重組行t a克隆，並進行dna序列分析，證明所克隆基因為mompdna序列。
  19. We applied the learning local languages to dna sequence analysis and also abtained good experimental results

    我們探索把持續局部可測語言用於血紅蛋白dna序列的識別，也獲得了不錯的實驗結果。
  20. Due to their systematic reliability, the dna sequence analysis, and the practical value of the radar exploration, the distribution in statistics becomes the current focus

    由於它們在系統可靠性、 dna序列分析以及雷達探測方面的應用價值，這些統計量的分佈問題是目前的一個熱點問題。
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