peptide chain 中文意思是什麼
peptide chain
解釋
肽鍵-
The enzyme of microbial transglutaminase ( mtg ) can modify the structure and properties of proteins through catalyzing an acyl transfer reaction of a - carboxyamide group of glutamine residue in a peptide chain, resulting the cross - linking of proteins
摘要微生物谷氨酰胺轉胺酶（ mtg ）可通過催化蛋白質谷氨酰胺的酰基轉移反應改變蛋白質的結構和性質，是一種有廣泛應用前景的重要新型酶制劑。 -
It is known from studies using brefeldin a ( bfa ) that disruption of tgn can block the cytotoxicity of ricin, suggesting that the intracellular compartment may be an important part of the uptake pathway. ricin enters the cells by receptor - mediated endocytosis, followed by translocation across the membranes of intracellular organelles. in this study, a trans - golgi retention peptide signal yqrl was fused to the c - terminus of ricin a chain ( rta ) by polymerase chain reaction
本實驗為重點研究反式高爾基體在毒素細胞內轉運過程中的作用，我們設計了一個反式高爾基體的靶向信號肽基因yqrl （ tyr - gln - arg - leu ）連接在rta分子的羧基端，用原核表達蛋白體系，純化並測定rta - yqrl與rta蛋白對hela （人宮頸癌細胞） ， wish （人羊膜上皮細胞） ， skov - 3 （人卵巢癌細胞）三種細胞的毒性作用。 -
The study of in - situ construction of cytocompatible surface on pdl - la matrix via amphiphile - amino acid ( rgd ) hybrid self - segregation - the amphiphilic diblock copolymer, poly ( dl - lactide ) - poly ( ethylene oxide ) ( pla - peo ) copolymer, containing hydrophobic pla block and hydrophilic peo block was synthesized via coupling method in this dissertation. cell - adhesion - promoting amino acids and integrin receptor peptide rgd were then immobilized at the end of peo chain of pla - peo copolymer via hydroxyl group activation technique. the solvent blending and casting method was then used to obtain the amphiphile modified pdl - la membranes
兩親共聚物-氨基酸（ rgd ）雜化體原位自修飾構建聚乳酸細胞相容性表面的研究一本論文首先設計併合成了一類含疏水聚乳酸（ pla ）鏈段和親水聚氧乙烯（ peo ）鏈段的兩親嵌段共聚物材料（ pla - peo ） ，利用peo鏈端的活性官能團羥基固定了促細胞粘附的氨基酸及整合素配體多肽片段rgd 。 -
Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies
小鼠噬菌體抗體庫的構建和n -肽結合單鏈抗體的篩選 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。 -
The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template
應用逆轉錄-聚合酶鏈式反應（ rt - pcr ）技術克隆得到編碼草魚生長激素（ cgh ）的基因cdna ，並定向克隆到puc18載體上。 -
There are 4 cysteins in the protein hbfgf, cysteins 78 and 96 are localized on the surface of hbfgf, whereas cystein 34 is completely buried and cystein 101 is partly buried within the folded peptide chain. so the cys78 and cys96 are especially prone to intramolecular disulphide - bond formation. we converted these two cysteins into serines
同時對於155個氨基酸的hbfgf來說，其34 、 78 、 96和101位的四個半胱氨酸的巰基有形成二硫鍵的可能，是造成二聚體或多聚體的主要原因，其中cys34隱藏在分子內部， cys101部分包埋在分子中； cys78和cys96暴露在蛋白質的表面，很容易因cys78和cys96的游離巰基氧化，在不同的分子間形成錯誤的二硫鍵。
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