recombinant vaccine 中文意思是什麼

recombinant vaccine 解釋
基因工程疫苗
  • recombinant : n. 【遺】重組器官，復合器官。
  • vaccine : adj. 牛痘的；預防疫苗的；種痘的。n. 1. 疫苗，牛痘苗；菌苗。2. 【計算機】免疫程序軟體，抗病毒軟體。
  1. Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method

    鑒于eo蛋白在病毒感染，誘導機體免疫及與宿主細胞相互關系中的作用，本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析，揭示了我國豬瘟病毒流行株之間的遺傳演化關系，為豬瘟病毒的流行病學研究提供依據。
  2. Healthy rabbits were inoculated each with vaccine of the recombinant fused pili - el - 2 protein at 250ul per dose to potentiate the immunogenicity. rabbits inoculated two times at one week interval

    擴增此質粒，回收bamh切出的2 . 5kb的基因片段，與pme290 （ bamh酶切後去磷酸化）載體連接。
  3. By using aa 385 - 365 fragment, an elisa system for the evaluation of post - e2 - vaccination humoral immune responses was also established, and was successfully applied to recombinant vaccinia virus - and dna - based vaccine research

    利用aa385 - 565片段，建立了e2疫苗免疫后抗體反應的elisa評價體系，並已成功用於重組痘苗病毒疫苗和dna疫苗的研究。
  4. Detection of prrs virions in the main organs of the challenged animals showed that, all recombinant plasmids and the inactivated vaccine failed to prevent the occurring of viremia

    而且不同的基因疫苗在誘導機體免疫應答、在機體的抗病毒作用中表現出不同的效能。
  5. The blood of each rabbit was collected at days 0, 7, 14, 2l, 30. the antibody titers were evaluated by k - agglutination test. the results showed that lower agglutination titers was observed at day 7 and up to higher levels over 8000at day 21 induced by the vaccine of recombinant fused protein

    將融合基因工程菌株在營養肉湯中培養，離心後上清用0 . 1mmgcl _ 2沉澱，離心，提取表達產物。經sds - page電泳證明表達產物為纖毛蛋白與il - 2的融合蛋白。
  6. This paper is a study on the expression of the protective gene of bont / a in the prokaryotic and eukaryotic. it indicates the possibility to produce the recombinant protein in quantities. it has also laid down a good foundation for the further research on vaccine or antibody of bont / a

    本論文進行了人工合成的a型肉毒毒素hc段基因在原核和真核表達系統中的表達研究，使制備大量bont a保護型抗原的重組蛋白成為可能，為進一步進行a型肉毒毒素的疫苗或抗體研究奠定了一定的基礎。
  7. Proteins of expression in multi - copy recombinant were able to react with monkey - anti - pzp3 a antibody and rabbit - anti - hcg antibody specifically. cone i us i on : recombinant pzp3 a hcg p - ctp109 - 145 was constructed and expressed in the yeast pichia pastoris successfully. it provides a foundation for further contraception vaccine research

    結論：重組質粒ppic9k - pzp3 - hcg - ctp構建成功，並在巴氏畢赤酵母中獲得重組抗原pzp3 - hcg - ctp的表達，為探討重組多特異性避孕疫苗抗生育效能的研究建立基礎。
  8. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  9. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  10. The result shows that a vvibdv strain was obtained, the above work lay a important role for further studying on the molecular biological mechanism of antigenic drift and virulence variation of ibdv, molecular epedimiology, it also provided the basis for recombinant and gene deleted vaccine of ibdv

    本實驗可以幫助我們進一步探討ibdv抗原性漂移和毒力變化的分子生物學機制，追溯ibdv的起源，理解病毒的傳播方式。同時也為研製開發基因重組疫苗和缺失疫苗打下一定的基礎。
  11. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  12. Construction and identification of recombinant attenuated salmonella typhimurium vaccine strain expressing helicobacter pylori urease subunit b

    重組減毒鼠傷寒沙門氏菌的構建和鑒定
  13. Construction and identification of a recombinant attenuated salmonella typhimurium vaccine strain expressing helicobacter pylori hpaa gene

    亞單位的重組減毒鼠傷寒沙門菌核酸疫苗的構建
  14. 3 immunogenicity of m3m4 loop recombinant peptide antigen of nr1, neuroexcitotoxicity protective effects and their mechanisms study of antiserum against m3m4 ( 1 ) to explore the immunogenicity of m3m4 loop recombinant peptide vaccine, m3m4 recombinant peptide was injected subcutaneously to immunize balb / c mice ( h - 2 ), cd4 / cd8 t lymphocytes subsets, the cytokine levels in serum and the liter of specific humoral response were detected with facs, indirect elisa and elisa separately

    ，抗血清還可以結合合成膚nps ，證明該表位具有免疫原性和抗原性。收集抗血清用於功能檢測。 （二） m3m4片段抗血清抗興奮毒性損害作用以臺盼藍染色法和原位末端標記（ tun 』 el ）法觀察抗血清對原代培養海馬神經元興奮毒性壞死和凋亡的保護效應。
  15. Study on the safety and immunogenicity of enterotoxigenic escherichia coli recombinant active vaccine

    腸毒素大腸埃希菌載體活菌疫苗的安全性和免疫性研究
  16. Construction of a recombinant attenuated salmonella typhimurium nucliec acid vaccine carrying helicobacter pylori hpaa and detecton of its immunogenicity

    減毒鼠傷寒沙門菌核酸疫苗的構建及免疫原性檢測
  17. Preliminary study on immunotherapy of an oral recombinant dna vaccine of helicobacter pylori neutrophil activating protein

    幽門螺桿菌中性粒細胞激活蛋白核酸疫苗實驗研究
  18. Influence of cpg - odn on immune effects of hepatitis b dna vaccine and recombinant hepatitis b vaccine

    對乙肝核酸疫苗及重組疫苗免疫效果的影響
  19. So, recombinant vaccine of hp ( rhp } was used in this study. it has been proved that vaccine administered by mucosa can produce effective immune response and prevent or cure hp infection

    幽門螺桿菌在粘膜部位感染機體，研究證實， hp疫苗經粘膜途徑接種能產生有效的保護性免疫應答，發揮預防、治療hp感染的作用。
  20. The target protein purified by ni - nta metal chelate affinity presented one major band in the sds - page and reacted with rabbit serum raised against the ndv b95 strain in western blotting, suggested that the protein could be used as one of candidated antigenes. it provided the basis for further studying on recombinant vaccine against ndv

    經westem - bloting雜交實驗，純化出目的蛋白與兔抗ndvb95株抗體反應，說明表達產物確為ndvb95株hn蛋白，且具有一定的免疫原性，為進一步研究重組疫苗創造了一定的條件。
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