recombinant virus 中文意思是什麼

recombinant virus 解釋
基因重組后的病毒
  • recombinant : n. 【遺】重組器官，復合器官。
  • virus : n 1 【醫學】病毒；濾過性病原體。2 毒素；毒害。3 惡意，惡毒。4 【計算機】（電腦）病毒〈指擾亂或破...
  1. Expression and genetic immunization of hantaan virus g2 recombinant adenovirus

    2重組腺病毒的表達及其基因免疫的研究
  2. The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus

    另外，通過共轉染和篩選純化獲得了攜帶appa基因的重組家蠶桿狀病毒。
  3. Pupal hemolymph infected with recombinant virus bmpak - hbmp and bmpak - hbm showed four protein bands of about 31, ' 30, 28 and 25 kd in the western blotting profiles, due to the two atg initial codons at the 5 " ends of both pres2 and s gene

    Western雜交結果表明，感染bmpak hbmp和bmpak hbm的家蠶蛹血淋巴中均有4種不同分子量的蛋白質和鼠抗人的一抗發生免疫反應，大小約分別為31kd 、 30kd 、 zskd 、 25kd 。
  4. After that we determined the presence of angiostatin gene in the putative recombinant virus with pcr analysis

    之後利用pcr分析是否獲得重組angiostatin的桿狀病毒。
  5. Hi our study, dendritic cells ( dcs ) were derived from the cultivation of peripheral blood monocytes in vitro successfully. then to observe whether dcs transfected with carcinoembryonic antigen ( cea ) - vaccinia recombinant virus ( rv - cea ) can induces cytotoxic t lymphocyte - mediated cea - specific immunity in vitro

    體外成功地完成了cd14 +單核細胞來源的樹突狀細胞（ dc ）的培養，並進一步研究人癌胚抗原重組痘苗病毒（ rv - cea ）轉染dc后體外誘導的cea特異性細胞免疫。
  6. Furtherly expression phase analysis showed that orf2 appeared in different form during infection. to reveal the function of haorf2, vha - orf2 - gfp recombinant virus which tagged with egfp - orf2 fusion protein was generated

    構建了ha2與綠色熒光蛋白融合表達的重組病毒vha - orf2 - gfp ，對該重組病毒感染細胞后，融合篡碩士學位論文mast卜r 』 s 』 r拼ifs
  7. Biological activity was determined by egf dependent balb / c 3t3 cell line and with mtt colorimetric assay. extracts of the recombinant virus - infected and mock - infected cells, haemolymph of the recombinant virus infected and mock - infected silkworm larvae could all support the proliferation of balb / c 3t3 cell. this phenomena implied that there were some egf - like growth factors in the haemolymph of normal silkworm larvae, which could enhance the proliferation of the cell line

    用小鼠balb c3t3成纖維細胞和mtt法測定表達產物的促細胞增殖作用，發現重組病毒感染家蠶細胞72小時的胞內樣品與正常家蠶細胞裂解物，以及重組病毒感染4天的蠶血淋巴與正常蠶血淋巴均具有相似的促細胞增殖作用，甚至野生型病毒感染的細胞裂解物和蠶血淋巴也有一定的細胞促生長作用，提示家蠶系統本身可能含有能促進細胞生長、類似於egf的細胞因子。
  8. The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting. the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized )

    用重組病毒bmpak - hbmp和bmpak - hbm [帶有非融合乙肝表面抗原（ pres2 + s ）基因，為本實驗構建]感染家蠶細胞及蛹，對兩種病毒的表達產物用elisa進行了跟蹤檢測，結果表明，感染bmpak - hbmp的家蠶細胞及蛹中rhbsag的表達量分別為3
  9. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  10. The purpose of this study is to construct a recombinant pseudorabies virus expressing ha gene of swine influenza virus and to develop specific diagnosis for differentiating the recombinant virus immunized animals and those naturally infected with prv or sfv

    本試驗的目的是以ge基因缺失的prv為載體構建可以表達sivha基因的重組病毒疫苗用於預防這兩種病，同時，基於此重組病毒的特點，建立可以區分此重組病毒疫苗和prv及siv自然感染的鑒別診斷方法。
  11. The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock

    構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。
  12. A deletion recombinant virus ( hasnpva132 ) of hal32 was generated by homologous recombination in e. coli. electron microscope pictures revealed the deletion virus could replicate in hzaml cells, which indicates hal32 is not essential for the replication of hasnpv

    通過在大腸桿菌內同源重組構建得到orf132的缺失重組病毒（ hasnpv凸132 ） ，轉染成功后的電鏡切片表明， hasnpv凸132能夠在hzami細胞內正常復制、增殖。
  13. Once the recombinant virus has been i - dentified, we amplified the p - 1 stock to attain the large scale, high - titer viral stock in order to initiate expression studies. the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared. the time course for expression of recombinant protein was detected by sds - page and western blot, which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein

    表達重組蛋白angiostatin ：用制備好的帶有矗s標記的融合性angiostatin基因的高滴度重組桿狀病毒貯存液感染草地貪夜蛾sfg細胞，用sds page電泳和hsternblot對感染不同時間後分泌的重組蛋白做時間表達分析，依此確定最適的感染復數（ moi ）和感染時間，以達到重組蛋白表達水平最適化，而後大規模進行重組蛋白的表達， sds page用來分析重組蛋白， westernblot用來在蛋白表達水平低的情況下檢測表達的特異重組蛋白。
  14. The expression of ha in vero cells infected with rprv - ha was detected by western - blot. the results indicated that ha protein could be consistently detected from a serial passages of vero cells infected with rprv - ha. the recombinant virus can be further developed as a live vectored vaccine against pseudorabies and swine influenza

    結果表明所獲得的重組病毒（ rprvha ）遺傳性狀穩定，在培養細胞中能穩定的表達與sivha具有相似生物學活性的外源蛋白，為進一步制備抗豬流感的重組偽狂犬病毒活載體疫苗奠定了基礎。
  15. The polyhedrin gene sequence in recombinant virus bmpak - hbmp can be more benefit to enhancing the expression of the hbsag ( pres2 + s ) from atg initial codon at the 5 " ends of pres2 gene

    另一方面， elisa檢測表明， presz抗原性提高率約是hbsag的14倍，提示bmpak hb帥中多角體基因部分堿基序列的存在更有利於從presz前的atg開始表達中蛋白全基因（ presz s ） 。
  16. To solve this problem, we used bac - to - bac system to recombine gfp - actin fusion gene and gfp gene under the control of the promoter of polyhedrin gene respectively. two recombinant virus : acmnpv - gfp - actin which contains gfp - actin fusion gene and acmnpv - gfp which contains gfp gene were obtained. the latter was set as a control

    為了解決以上問題，在以上實驗的基礎上，我們利用bac - to - bac系統分別將gfp基因和gfp - actin融合基因重組于多角體基因啟動子之下，構建了兩種重組病毒，即含gfp - actin融合基因的重組病毒acmnpv - gfp - actin和含gfp基因的重組病毒acmnpv - gfp 。
  17. Then bm cell line was co - transfected with parental bm - npv dna and the transfer plasmid pvl - nk, the recombinant virus was then obtained subsequently with routine procedure. nattokinase was expressed in silkworm larva by injection with the recombinant virus

    將nk基因克隆至轉移載體pvl - 1393中後用常規方法獲得重組病毒，感染家蠶后納豆激酶獲得正確表達。
  18. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
  19. The recombinant virus adhuctla4 - ig prepared in this study efliciently inltcted l - o2 " cells, and the infected cells expressed a - nd excreted soluble rccolllbina11t protein huctla4 - ig

    該重組病毒在體外能有效感染正常肝細胞株l - o2 ，受感染細胞能表達、分泌可溶性的重組融合蛋白huctla4 - ig ； 2
  20. The bmn cells and fifth instars were infected by the recombinant virus bacpak - angiostatin. the bioactivity of the protein product was determined by human umbilical vein endothelial cells ( ecv304 ) growth assay in vitro and inhibition of vascular growth assay in cam in vivo. angiostatin showed significantly inhibitory effect on endothelial cells

    重組病毒以moi = 10感染家蠶細胞（ 2 10 ~ 6個細胞/ 5ml ）和家蠶5齡幼蟲，表達產物用體外培養的人臍靜脈血管內皮細胞（ ecv304 ）及體內雞胚尿囊膜（ cam ）新生血管實驗檢測其抑制活性： （一）血管抑素可明顯抑制體外培養的內皮細胞增殖。
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