rna primer 中文意思是什麼
rna primer
解釋
rna引子-
Total rna was extracted from the second stage larve of hypoderma sp, then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer. the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software
本試驗的目的旨在進行hypodeminc （ hc ）和hypodermina （ ha ）基因的克隆、測序、構建重組表達載體並誘導表達，獲得重組抗原，以解決天然抗原的不足並為診斷和免疫試劑的產業化奠定基礎。 -
One version of the dideoxy sequencing method can be used to sequence the 5'ends of rna molecules by "primer extension".
一種形式的雙脫氧序列測定法是通過引子的延長來測定RNA分子5』末端的序列。 -
Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。 -
To device a primer pairs and amplify the full length pstvd by rt - pcr, the positive rna extraction from tuber sample was used as template. the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
Cdna核酸斑點雜交反應（ nash ）檢測pstvd方法準確、靈敏度高，一次檢測樣品數量多，且對于異地樣品檢測非常方便，是以往其它檢測方法的有效補充。 -
In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。 -
The procedure of ddrt - pcr applicable for silver staining was optimized by adjusting the amount of several critical reagents, including total rna, anchor primer, arbitrary primer, cdna and dntp
通過調整ddrt - pcr中總rna 、錨定引物、隨機引物、 cdna和dntp等關鍵試劑的用量，優化了適用於銀染檢測的ddrt - pcr方法。 -
Total rna was extracted from hepatic cells of mouse. a ecori and bamhi restriction sites were introduced into endostatin gene at specific primer f r, and endostatin was amplified by rt - pcr, this endostatin gene contained bamhi and ecori restriction sites at its 5 " and 3 " ends respectively
從小鼠肝臟細胞中提取總rna 。設計合成一對特異引物，分別帶有ecori和bamhi的限制性內切酶的識別位點。用rt - pcr法擴增endostatin的基因片段，在endostatin基因的兩側引入ecori和bamhi酶切位點。 -
Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method
首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。 -
One version of the dideoxy sequencing method can be used to sequence the 5 ' ends of rna molecules by " primer extension "
一種形式的雙脫氧序列測定法是通過引子的延長來測定rna分子5 』末端的序列。 -
A novel hybridization capture - pcr elisa was established for pnrsv detection, the main contributions to the development of the method are that a solid primer is successfully bound to pcr tube wall specially for aimed rna capture in order to raise rna purification and reduce time consumption
固相引物既作為誘捕鏈參與核酸的誘捕，又參與pcr擴增，擴增中，在taq酶的作用下，擴增產物沿著該引物延伸，形成一條固定在管壁上的鏈。
分享友人