upstream sequence 中文意思是什麼
upstream sequence
解釋
上游序列-
The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned, sequenced and characterized by using chromoseme walking method. the sequence includes cpcb gene ( 519 bp ), cpca gene ( 489 bp ), cpch gene ( 357 bp ), and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ), 111 bp of phycocyanin intergenetic spacer ( pc - igs ). upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e. coli, synechocystis pcc 6803 and a. platensis fachb341 by supersonic and electrophoresis methods
根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻（ arthrospiraplatensisfachb341 ）藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列（ pc - igs ）並進行序列測定，然後根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因（ cpcb ， 519bp ） ，亞基基因（ cpca ， 489bp ） ，連接蛋白h基因（ cpch ， 357bp ） ，亞基基因上游啟動子序列（ 427bp ）以及各基因之間的間隔區（ pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ） 。 -
Promotor, in broad sense, consists of transcription start site, binding site of rna polymerase ( promoter in narrow sense ) and upstream regulation sequence
廣義的啟動子（ promotor ）包含有轉錄起始部位、 rna聚合酶與dna結合部位（也就是狹義的啟動子）以及上游調控序列。 -
4, an intron sequence was also inserted upstream of gfpmut3 and its six reading frame could all be stopped, which could guarantee gfp translation in right reading frame
將藍色熒光蛋白基因bfp克隆到pet - 11c上，轉化bl21 （ de3 ）后實現了bfp在大腸桿菌中的誘導表達。 -
In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。 -
( 3 ) on the basis of the deletion analysis, three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element. transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation. loss - of - function experiment, using transient expression analysis of gus reporter genes, confirmed that gcc box act as an essential element to respond ja signaling in pdf1. 2 promoter
（ 3 ）在缺失突變的基礎上，通過對gccbox及其相鄰的上下游六個堿基進行取代突變，將突變啟動子與gus構建融合基因，在煙草中受heja誘導的瞬時表達結果表明， h1和m3的突變對該啟動子應答ja信號的影響很小，而m2 （ gccbox的突變）則幾乎使該啟動子應答ja信號的功能完全喪失，所以gccbox是該啟動子中應答ja信號的必需元件。 -
The most efficient regulation of gene occurs at transcription level by regulating the interaction between transcription factors and upstream regulation sequence. thus, to investigate promoter of a target gene will be helpful to predicate the principle of molecule regulation, biological function of molecule and even involving pathogenesis of some diseases
轉錄水平是調控蛋白質表達效率最高的環節，通過影響相應的轉錄因子與啟動子和上游調控序列的相互作用調控目的基因的表達，因此研究基因的啟動子對于了解基因的表達調控規律、闡明分子的結構和生物學功能乃至疾病的發生都有重要的意義。 -
There is typical - 10 region, - 35 region and rbs sequence on the upstream of orf1 and on the upstream of the - 35 region there are four tandem repeats of 22 bps sequence, and similar sequence was found on the upstream of the replicase genes of pmbbl, pvs40, plu510
在orf1上游具有典型的- 10區、 - 35區和rbs堿基序列， - 35區上游附近四段22bp正向串聯重復序列，相類似的序列也見于質粒pmbb1 、 pvs40 、 plq510復制蛋白酶基因上游。 -
The 2. 0 - kb bamhl - sphl fragment, upstream fragment of the apramycin resistant gene, was sequenced. analysis of the nt sequence revealed that it contained an orf from nt positions 877 to 1995
抗性基因上游2 . 0kbbamhi - sphi片段測序表明在該片段中有一個完整的orf ，位於877 1995bp之間，編碼373個氨基酸。 -
The 3. 4kb ecori dna fragment of the pgxn201 and the mutant ecori dna fragments of the three mutant plasmids were subcloned into the ecori sites of the clone vectors ml3 and the pgem3z - f ( + ) respectively. through dna sequencing and dna sequence analysis by comparing with the bradyrhizobium japonicum dna sequences reported, we found that there are four intact open reading frames in the 3. 4kb fragment and it was highly homologous to the bradyrhizobium japonicum dna sequence reported, and the tn5gusa5 containing in the pgxn217 was inserted in one of the four open reading frames. this open reading frame located on about 5. 5kb upstream of the nfec gene, which includes 207bp nucleotides, coding 68 putative amino acids and this is completely identical to that of the bradyrhizobium japonicum dna sequence reported
本研究首先利用轉座子tn5gusa5對重組質粒pgxn201進行誘變，獲得3 . 4kbecori片段插入了tn5gusa5的突變質粒pgxn215 、 pgxn216 、 pgxn217 。對這些突變質粒進行亞克隆及測序分析並與基因庫中已報道的慢生型大豆根瘤菌的dna序列作比較，發現突變質粒pgxn217中tn5gusa5恰好插入在一個未知功能的開放閱讀框架內。將pgxn201的3 . 4kbecori片段與m13作連接，獲得亞克隆pgxn201c ，對pgxn201c進行測序分析，發現與基因庫中已報道的慢生型大豆根瘤菌的dna序列有95的同源性。 -
Two crtw 5 ' - flanking upstream sequences ( 795bp and 695bp, respectively ) and one crtz 5 ' - flanking upstream sequence ( 302bp ) are obtained using ada ptor - primer pcr method
分離到兩個crtw5 '上游序列和一個crtz上游序列，長度分別為795bp 、 695bp和302bp 。
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