內肽酶酶 的英文怎麼說
中文拼音 [nèitài]
內肽酶酶
英文
endopeptidase-
Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史,大致經歷了死疫苗、活疫苗(照射致弱尾蚴疫苗)和基因工程疫苗等研究階段,產生了一些who / tdr推薦認為很有希望的疫苗候選分子,如谷胱甘肽- s -轉移酶( gst ) 、副肌球蛋白( sm97 ) 、照射致弱疫苗抗原5 ( irv - 5 ) 、磷酸丙糖異構酶( tpi ) 、曼氏血吸蟲膜內在蛋白( sm23 )和脂肪酸結合蛋白( fabp , sm14 )等,但其對宿主的保護作用均不甚理想,未能穩定地達到40或以上的保護力水平,因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑,進一步提高其保護力。Cultivation optimization of prolyl endopeptidase and high cell density fermentation
脯氨酰內肽酶培養條件的優化及高密度發酵Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4
Sds page分析得到3條主要蛋白帶,剪下這三條帶進行膠內賴氨酸內切酶的消化,通過高效液相色譜分離肽段,選擇性進行肽段的氨基酸序列測定。The changes of these endopeptidase are different during the former vasing of " samatha " and " belami ". the main eps in petals of " samatha " are the acid thiol - proteinase, metallo - proteinase and the alkali serine proteinase and the main eps in petals of " belami " are the acid thiol - proteinase and serine proteinase. during the later vasing the acid metallo - proteinase and the alkali serine proteinase become main eps in petals of " samatha " and " belami "
這3種內肽酶在兩種切花瓶插初期(盛開以前)的表現不同: 『薩蔓莎』花瓣中以酸性金屬蛋白酶、巰基蛋白酶和堿性絲氨酸蛋白酶起主要作用, 『貝拉米』花瓣以酸性巰基蛋白酶、絲氨酸蛋白酶起主要作用,後期『薩蔓莎』和『貝拉米』花瓣均以酸性金屬蛋白酶、堿性絲氨酸蛋白酶起主要作用。The main study in this paper included as follows : the content and distribution of heavy metals in sediments and benthic organisms from the sewage stream in guangzhou city ; the acute toxicity and joint toxicity of mercury and selenium to swordtail fish ( xiphophorus helleri ) ; the damage of mercury to the indexes of antioxidant system in the gills and livers in swordtail ( including the measurement of the activities of total antioxidative capacity [ t ~ aoc ], superoxide dismutase [ sod ], glutathione peroxidase [ gsh - px ] and the concentration of malondiald - ehyde [ mda ] ) and the relief effects of selenium on it, as well as the physiological damage of mercury on the tissues, namely : the antagonistic effect of na + - k + ~ atpase activity on the tissues between selenite and mercury, and the ultrastructural damage under the exposure of mercury
研究內容主要有:廣州市河涌沉積物及底棲生物體內重金屬含量及評價;汞和硒對劍尾魚的急性毒性和聯合毒性及安全濃度的評價;汞對劍尾魚鰓和肝臟中抗氧化系統的毒性,包括對總抗氧化能力、超氧化物歧化酶、谷胱甘肽過氧化物酶活力及丙二醛含量的測定及硒對其保護作用;汞對劍尾魚組織生理毒性即:汞對na ~ + - k ~ + atpase活力的影響及硒的保護作用和汞和對劍尾魚組織超微結構的損傷等。以高氯酸?硝酸消化法和火焰原子吸收分光光度法測定了廣州市河涌沉積物和底棲生物中重金屬含量。The extracted marrows ganoderma lucidum and semen zizyphi spinosae contained in threesss can regulate secretion of hydrocortisone in human body and reduce excitation of human body during nighttime ; in addition, ganoderma - lucidum spores and semen zizyphi jointly stimulate secretion of sleeping - inducting peptide in body. improve rate of " high efficient sleeping quality and immunity. recover and strengthen mental strength, mental strength and vigor. through sleeping. the psychlogy and physiology return to the youth level
「索萊爾」多元明膠囊中所含的靈芝和酸棗仁提取精華,能調節人體內皮質醇的分泌,降低人體在夜間的興奮度;此外靈芝孢子粉和酸棗仁共同作用,能夠在體內刺激「睡眠肽」和格里酶「的分泌,有效促進「高能睡眠」率,延長「高能睡眠」時間,從而達到全面改善睡眠質量,提高免疫力,使體力、腦力、精力得到迅速恢復和加強,通過睡眠將生理及心理狀態調整到青春期水平。The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide
核苷酸序列分析表明, pcr擴增產物中包含有完整的phya基因,該基因全長1506bp ,其中包含一段長102bp的內含子,該內含子具有真菌植酸酶基因內含子的特徵保守序列: donor序列? gtatgc , lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸,理論分子量為51 . 37kda ,其上有13個潛在的n -糖基化位點, n端19個氨基酸為信號肽序列,植酸酶活性位點序列( crvtfaqvlsrhgaryptdskgk )位於氨基酸序列的+ 71 + 93 。Casein of dairy is the important sources of those peptides and can release them during gastronintestinal digestion, or enzyme proteolysis
摘要酪蛋白是生物活性肽的重要來源,可以通過體內的胃腸消化和食品加工過程中的酶解將其釋放出來。This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected
本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;並在合成、純化後分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。2. the optimum temperature of eps is 37 ' c in petal and leaf of " samantha " and " belami "
兩個品種切花花瓣、葉片中,內肽酶最適溫度均為37 。Relationship between polyamines and ethylene evolution of cut rose flowers during senescence
兩個切花月季品種花朵開放和衰老對乙烯的反應及其與內肽酶的關聯3. the changes of ep activities of petal are more complicated than those of leaf during vasing
在瓶插期葉片內肽酶活性變化平緩,花瓣內肽酶活性變化復雜。Enzyme ( polypeptide ) : the enzyme is a kind of organic catalyst created by the body of aloe, which is a kind of special protein
酶(多肽) :酶是蘆薈植物體內所產生的一種有機催化劑,是一種特殊蛋白質。There are at least three kinds of ep in petal : thiol - proteinase, metallo - proteinase, serine proteinase. they gather at the site between 43 kd and 66. 2 kd ( about 52 kd ) on the substrate gel electrophoresis. the optimum ph of thiol - proteinase, metallo - proteinase is ph 6, which are looked as acid ep, the serine proteinase has two forms, one is acid ep ( ph 6 ), and the other is alkali ( ph 10 )
花瓣中都至少有3種內肽酶:巰基蛋白酶、金屬蛋白酶、絲氨酸蛋白酶,三者聚集在底物膠電泳膠條的43kd 66 . 2kd之間的位置,約為52kd ;前兩種最適ph為6 ,是酸性內肽酶,后一種有酸( ph6 ) 、堿( ph10 )兩種存在形式,既是酸性內肽酶又是堿性內肽酶。There are mixed reports regarding the status of glutathione and glutathione enzyme activities in psoriatic patients but a recent paper by kokham and naziroglu indicates that glutathione levels and glutathione peroxidase activity in plasma and red cells is significantly lower in these subjects ( 3 )
有許多的報告論及牛皮癬患者的谷胱甘肽和谷胱甘肽酶活動的狀態, (研究3 )指出患者血漿及紅血球內的谷胱甘肽水平和谷胱甘肽過氧化酶活動顯著較低。Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site, and the igf - i product of pcr contains 230 base pairs. igf - ii contains 219 base pairs. 3
各另外設計一對特異性pcr引物,導入適當限制性內切酶切點,以上述連有目的基因的克隆載體為模板,採用pcr方法擴增基因片段,獲得長度約230bp的igf -和219bp的igf -成熟肽基因序列。The content of soluble protein is also increased after deficit water stress, but the increasing of " belami " is more significant than that of " samantha ". 5. after deficit water stress, in " samantha ' petals, the metallo - proteinase activities of the optimum ph 6 and the serine proteinase activities of the optimum ph 10 are increased markedly, the thiol - proteinase activities of the optimum ph 6 is decreased markedly
失水脅迫后顯著提高了『薩蔓莎』花瓣中最適ph6條件下金屬蛋白酶、最適月季( rosahybrde )切花瓶插期間內肽酶與衰老的關系及失水脅迫對其影響的研究ph條件下的絲氨酸蛋白酶的活性,顯著降低了最適ph6條件下琉基蛋白酶的活性。From beginning to end, the ep activities of the optimum ph 10 of " samatha " petal is higher by far than added values those of the optimum ph 6 and the optimum ph 10 of the " belami " petal. this is one of likely reasons that " samatha " can open quickly and fully during vasing
整個過程中『薩蔓莎』花瓣最適ph10條件下的內肽酶活性遠大於『貝拉米』最適ph6 、 ph10條件下的內肽酶活性的疊加值,這可能是『薩蔓莎』瓶插期花朵能充分迅速綻開的原因之一。6. there are very close relations between the rapidly decreasing of the soluble protein content, the sweetly enhancing of ep activities, the quckly increasing of fre
切花瓶插期間,花瓣、葉片中可溶性蛋白質含量迅速降低、內肽酶活性迅速增強、游離氨基酸含量迅速升高與切花衰老密切相關,可以作為切花開始衰老的生理指標。In order to seek the probable reasons " of difference of the process of opening to senescence between " samantha " and " belami ", the relations between senescence and ep were probed. the flowing results were obtained : 1
探討了切花月季開花進程不同的品種瓶插期衰老與內肽酶的關系,以尋找引起它們開花進程不同的可能原因,主要得出了以下結果。分享友人