寡基因的 的英文怎麼說
中文拼音 [guǎjīyīnde]
寡基因的
英文
oligogenic-
In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover
本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins
將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測，發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分，阿維菌素的總產量和b1的產量與出發菌株相當，說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。All kind of oligonucleotides ( 16 sense primers, 8 antisenset primers, 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna. no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations
依據nbs - lrr類r基因特有的兩個保守序列glpl和cfly （兩者相距約70個氨基酸）合成所有可能的簡並寡核苷酸引物（左引物16個，右引物8個，共組成128個引物組合）對玉米基因組dna進行擴增的結果表明：在6個引物組合中得到了擴增產物，產物長度在500 1000bp之間；沒有獲得特異性擴增產物（指僅從含ht基因材料中得到，長度約210bp的dna片斷） 。Prepare the oligonucleotide probes designed 16 probes as four groups according to hla - dqa1 sequence in genbank and modify each probes by ammonia at 5 ' - end. then resuspended the lyophilized probes in ph = 9, 0
寡核苷酸探針的制備根據genbank數據庫新近公布的hla - dqa1等位基因序列，設計四組共16條特異性寡核苷酸分型探針，並在各5 』端做氨基修飾。These interactions are primarily dependent upon the coordinated actions of ovarian progesterone and estrogen, moreover, many other factors, such as growth factors, cytokines, ecm, adhension molecules, oligosaccharides and proteases, regarded as local mediators, endometrium and embryo have also expressed some specific receptors, via intracellular signal transduction chains and express some key genes, making receptivity of the uterus and synchronized development of the embryo to the blastocyst stage
成功的植入是處于接受態的子宮內膜和具有侵入性的胚胎間的同步協調反應。植入過程受多種生長因子及其受體、細胞因子、粘附分子、蛋白水解酶、寡糖等的精細調控，通過胞內信號轉導及關鍵基因的表達使子宮內膜發生一系列復雜變化。Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan, and be stimulated by ifn - r before oligochitosan added, then measured the changes of gene transcription and translation level of both il - 1 and imf - a, respectively by methods of relatively quantitive rt - pcr and elisa. first, rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan, then by the same method, confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating. because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone, so add ifn - r to microphages alone for 22 hours, then examined by rt - pcr, the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group
此外，由於ifn y單獨作用也可促進兩種細胞因子基因表達，故在巨噬細胞中加入ifn y單獨作用22h ，再經阿一pcr檢測，發現加ifn y的實驗組細胞的幾一lp和tnf a基因轉錄水平與空白對照組相比較無顯著性差異，可見，殼寡糖和ifn v對巨噬細胞il lp和tnf一口基因轉錄水平的影響在作用時間上無一致性，在殼寡糖作用最適時間時，僅受ifn y刺激的巨噬細胞il lp和tnf q基因轉錄己下降至刺激前水平，因此可以認為， ifn y的加入僅起到對巨噬細胞預刺激使之處于敏感狀態的作用，有利於增強殼寡糖對巨噬細胞的作用。Then the effect of turning channel has been studied. 7. this detection system has been used to dna segments separation experiments ; the results showed that it has met the need for dna separation
將該微通道電泳晶元系統應用於不同熒光標記寡核苦酸、 dna片段的分離分析，實現了寡核著酸的單堿基分辨，同時應用於結核桿菌基因組pcr產物的分析，實驗結果表明此系統可以實現dna片段的快速高效分析。In this study, we attempted to construct an engineering strain producing only avermectin bl through the replacement of dna encoding dh2 - kr2 domains of the avermectin pks ( avedh2 - kr2 ) with dna encoding dh2 - kr2 domains from the pikromycin pks in s. avermitilis olm73 - 12, producing only avermectins b and no oligomycin. gene replacement vector pxl201 ( pkc1139 : : 5 ' flank + pia : dh2 - kr2 + 3 ' flank ) was used to transform 5. avermitilis olm73 - 12 protoplasts
我們以不產寡黴素而僅產阿維菌素b的工程菌olm73 - 12為出發菌株，用委內瑞拉鏈黴菌（ streptomycesvenezuelae ）中編碼pikromycinpks模塊2上完全活性的dh和酮基還原酶（ kr ）的dna區域對olm73 - 12染色體上編碼阿維菌素pks模塊2中dh和kr的區域進行取代，試圖構建僅產b1組分的基因工程菌。The key stage of fabricating gene chip is pretreatment of glass surface including the processes of nh3h2o treatment, aminosilane treatment and aldehyde treatment. the pretreatment can grow active group that can bind probe effectively on the surface of glass slide. as a result, the actively treated glass slide can suit for fabricating in - situ synthesis high density gene chips
基因晶元制備技術的關鍵步驟是玻片表面預處理，即對玻片表面進行羥基化、氨基化和醛基化處理，使表面生長的活性基團能有效固定寡核苷酸探針，以滿足原位合成高密度基因晶元對玻片的要求。An oligo - nucleotide primer was designed according to a conservative fragment of this gene of plants in genebank. two 3 ' - end coding sequences of this gene ( including complete sequences of exon 8, 7, 6, 5, 4 and partial sequences of exon 3 ), one, 1097bp, and the other 1109bp, were successfully isolated from brassicaeae napus and brassicaeae campestris. the dna sequence analysis shows that the two fragments are more than 90 % identical to orychophragmus violaceus, arabidopsis thaliana and brassicaceae napus
同源對比分析已有的植物epsps基因序列，我們找到了該基因中一段極為保守的序列並設計了一條寡核苷酸引物，採用3 』 race法，從植物蜀雜9號油菜，白菜型油菜總rna中成功的擴增出了epsps基因3 』端編碼區（包含外顯子8 、 7 、 6 、 5 、 4的全部序列及外顯子3的部分序列） ，分別長1097bp ， 1109bp 。A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments. two dna fragments were first cloned from o. violaceus by performing prc which used o. violaceus genome as the template. one has 798 nucleotides and the other 1157 nucleotides, but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron
根據同源比較其它高等植物中epsp合成酶基因，找出該基因的保守序列並設計一對寡核苷酸引物，以諸葛菜的總dna為模板進行pcr反應，克隆出了兩個epsps基因的片段，其中一條長為797bp ，另一條1157bp ，它們在genbank的登錄號為： af440390 、 af440391 。A pair of primers were designed according to the published sequence of gnrh2 ' s gene mrna and transporter gene with oligo version 4. 1 softwarre, they were annealed by their 3 ' terminal brief complementary base sequence to form small segment double chain, therery, they serve mutually as template and primer, and their extension were carried out to synthesize 90 bp " gnrh / trs
本研究根據genbank中已發表的人gnrh2基因mrna序列以及轉運肽（ transporter ， trs ）基因核苷酸序列，藉助oligo4 . 1設計了一對寡核苷酸引物，以引物3末端的短互補序列退火形成小段雙鏈，從而互為模板和引物，通過引導合成長達90bp的gnrh trs序列gnrh trs 。Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule
其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。Effect of et - 1 antisense oligodeoxynucleotide on the hemodynamics of normal and experimental hypertensive rats
內皮素- 1前體基因反義寡核苷酸對正常和高血壓大鼠血流動力學的影響Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected
Rapd （隨機擴增多態性dna ）技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術，它建立於聚合酶鏈式反應（ pcr ）技術基礎之上，利用隨機合成的寡聚核苷酸序列為引物（一般為10個bp ） ，分別與dna的兩條單鏈結合，在dna聚合酶的作用下，對基因組的特定區域進行pcr擴增，其電泳結果為不同大小和數目的dna譜帶即rapd圖譜，可反映基因組相應區域的dna多態性。For these goals, the fo llowing jobs have been done and some results have been obtained. 1 according to the vitreoscilla hemoglobin ( vhb ) amino acid sequence and plajnt preference codon usage, vgbm gene was designed and synthesized by annealing 22 synthetic fragments respectively, the modified vgbm gene of full length of 450 base pairs was synthesized. 2 the transformants were obtained after pbv221svhb was introduced into e. coli
為此本論文作了以下工作並得出了一些結論： 1根據透明顫菌血紅蛋白（ vhb ）的氨基酸序列，選用植物偏愛密碼子，對透明顫菌血紅蛋白基因（ vgb ）進行優化改造，設計併合成了22條寡核苷酸短片段，人工合成了改造的全長450bp的透明顫菌血紅蛋白（ vhb ）基因（ vgbmA reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。These results show that cdna expression library has an excellent quality and lays solid foundation for further screening. in addition, the library is also used for screening other breast cancer relevant genes
同時由於該文庫包含了此乳腺癌細胞系的全部基因信息，因此也可以用其他的抗體或寡核昔酸探針篩選乳腺癌的相關基因。分享友人