寡核 的英文怎麼說

中文拼音 [guǎhé]
寡核 英文
oligonucleotide
  • 寡 : Ⅰ形容詞1 （少; 缺少） few; little; scant 2 （淡而無味） tasteless; insipid Ⅱ名詞1 （丈夫死去並未...
  • 核 : 核構詞成分。
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. All kind of oligonucleotides ( 16 sense primers, 8 antisenset primers, 128 different primer combinations in all ) were designed from the conserved motifs glpl and cfly ( distance between them is approximately 210bp ) which existed only in the type of nbs - lrr resistance gene and used as primers for scanning the genomic dna. no specific products ( which is approximately 210bp in length and obtained from genomic dna containing ht gene ) was obtained though amplified products of approximately 500 - looobp appeared in six different primer combinations

    依據nbs - lrr類r基因特有的兩個保守序列glpl和cfly （兩者相距約70個氨基酸）合成所有可能的簡並寡核苷酸引物（左引物16個，右引物8個，共組成128個引物組合）對玉米基因組dna進行擴增的結果表明：在6個引物組合中得到了擴增產物，產物長度在500 1000bp之間；沒有獲得特異性擴增產物（指僅從含ht基因材料中得到，長度約210bp的dna片斷） 。
  3. Prepare the oligonucleotide probes designed 16 probes as four groups according to hla - dqa1 sequence in genbank and modify each probes by ammonia at 5 ' - end. then resuspended the lyophilized probes in ph = 9, 0

    寡核苷酸探針的制備根據genbank數據庫新近公布的hla - dqa1等位基因序列，設計四組共16條特異性寡核苷酸分型探針，並在各5 』端做氨基修飾。
  4. Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers

    嘗試應用多位點dna指紋技術，檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明：採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜，具有足夠的多態性和很好的穩定性。
  5. Inhibitory effects of heparinase antisense oligodeoxynucleotides on growth of human malignant melanoma trans planted subcutaneously in nude mice

    乙酰肝素酶反義寡核苷酸對人惡性黑素瘤裸鼠皮下移植瘤抑制作用的研究
  6. Then the effect of turning channel has been studied. 7. this detection system has been used to dna segments separation experiments ; the results showed that it has met the need for dna separation

    將該微通道電泳晶元系統應用於不同熒光標記寡核苦酸、 dna片段的分離分析，實現了寡核著酸的單堿基分辨，同時應用於結核桿菌基因組pcr產物的分析，實驗結果表明此系統可以實現dna片段的快速高效分析。
  7. The results of the experiments indicates that concentration of aminosilane influences the fluorescence background of glass slide, and some factors affect immobile ratio of oligonucleotide probe, such as aminosilane treatment time, aldehyde treatment time, uv crosslinking energy, washing temperature and time

    研究表明，氨基化試劑濃度對玻片熒光背景有影響，氨基化試劑處理時間、醛基化處理時間、紫外交聯能量和洗滌溫度和時間等工藝因素影響寡核苷酸探針的固定率。
  8. The key stage of fabricating gene chip is pretreatment of glass surface including the processes of nh3h2o treatment, aminosilane treatment and aldehyde treatment. the pretreatment can grow active group that can bind probe effectively on the surface of glass slide. as a result, the actively treated glass slide can suit for fabricating in - situ synthesis high density gene chips

    基因晶元制備技術的關鍵步驟是玻片表面預處理，即對玻片表面進行羥基化、氨基化和醛基化處理，使表面生長的活性基團能有效固定寡核苷酸探針，以滿足原位合成高密度基因晶元對玻片的要求。
  9. First, glass slides having been rinsed will be treated with nh3h2o, aminosilane and aldehyde. second, the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope. in the end, the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods

    實驗選用表面平整的德國玻片，將清洗好的玻片分別進行羥基化、氨基化、醛基化，採用熒光法和原子力顯微鏡法分別檢測玻片表面預處理質量，研究兩種檢測方法之間的內在聯系，從而確定表徵玻片表面寡核苷酸探針固定率的方法。
  10. The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin

    對照組培養液中不含g廿一1 （ 7一36 ） nhz ，實驗組培養液中含有20nmol / lglp一1 （ 7一36 ） nhz ，培養24h后，用0 . 25 %胰蛋白酶消化胰島分散細胞，塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交， dab顯色，高清晰度病理圖文分析系統（ highpathologiealimageanalysissystem ， hp認s ）對細胞著色的平均光密度（ mean即tiealdensity ， mod ）量化分析，觀察實驗組和對照組胰島素mrna的表達情況。
  11. Experimental study of tgf - 2 antisense oligodeo - xynucleotide as an anti - scarring agent in glaucoma surgery

    2反義寡核苷酸抑制抗青光眼術后濾過泡瘢痕的實驗研究
  12. An oligo - nucleotide primer was designed according to a conservative fragment of this gene of plants in genebank. two 3 ' - end coding sequences of this gene ( including complete sequences of exon 8, 7, 6, 5, 4 and partial sequences of exon 3 ), one, 1097bp, and the other 1109bp, were successfully isolated from brassicaeae napus and brassicaeae campestris. the dna sequence analysis shows that the two fragments are more than 90 % identical to orychophragmus violaceus, arabidopsis thaliana and brassicaceae napus

    同源對比分析已有的植物epsps基因序列，我們找到了該基因中一段極為保守的序列並設計了一條寡核苷酸引物，採用3 』 race法，從植物蜀雜9號油菜，白菜型油菜總rna中成功的擴增出了epsps基因3 』端編碼區（包含外顯子8 、 7 、 6 、 5 、 4的全部序列及外顯子3的部分序列） ，分別長1097bp ， 1109bp 。
  13. Reliable detection of rifampin - resistance of mycobacterium tuberculosis strains by using a specialized oligonucleotide microarray

    檢測耐利福平結核分枝桿菌寡核苷酸晶元的研製及應用
  14. A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments. two dna fragments were first cloned from o. violaceus by performing prc which used o. violaceus genome as the template. one has 798 nucleotides and the other 1157 nucleotides, but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron

    根據同源比較其它高等植物中epsp合成酶基因，找出該基因的保守序列並設計一對寡核苷酸引物，以諸葛菜的總dna為模板進行pcr反應，克隆出了兩個epsps基因的片段，其中一條長為797bp ，另一條1157bp ，它們在genbank的登錄號為： af440390 、 af440391 。
  15. A pair of primers were designed according to the published sequence of gnrh2 ' s gene mrna and transporter gene with oligo version 4. 1 softwarre, they were annealed by their 3 ' terminal brief complementary base sequence to form small segment double chain, therery, they serve mutually as template and primer, and their extension were carried out to synthesize 90 bp " gnrh / trs

    本研究根據genbank中已發表的人gnrh2基因mrna序列以及轉運肽（ transporter ， trs ）基因核苷酸序列，藉助oligo4 . 1設計了一對寡核苷酸引物，以引物3末端的短互補序列退火形成小段雙鏈，從而互為模板和引物，通過引導合成長達90bp的gnrh trs序列gnrh trs 。
  16. The result of fluorescence show that the fluorescence intensity of the surface of the treated glass slide connect with the probe immobile ratio of oligonucleotide. the more oligonucleotide probes have been linked with active group, the stronger fluorescence intensity is. for the strongest fluorescence, the technical conditions is : treatment of 2 % aminosilane of 20 minutes, treatment of 5 % aldehyde of 24 minutes, uv crosslinking of 150mj and washing of 5 minutes at 20

    兩種檢測方法表明，當活性基團呈柱狀、分佈均勻且尺寸比較大（ 200nm ）時，有利於寡核苷酸探針的連接，且連接探針數量多，玻片表面熒光強度強，固定率高；當活性基團呈錐狀、分佈及尺寸不均勻（ 150nm （ 300nm ）時，連接的寡核苷酸探針數量少，玻片表面熒光強度弱，固定率低。
  17. 5. prepare the oligonucleotide microarray add 16 prepared probes into 384 - pole plate, meantime, add p2, low homologic probes and probe buffer in the same concentration as positive control, blank control and negative control respectively. spot microarray as designed by the spotting machine

    5 .寡核昔酸微陣列的制備以同等濃度的反義鏈引物咫作為陽性對照，以探針緩沖液作為空白對照，以無關探針作為陰性對照，與制備好的16條寡核昔酸探針，同時加人384孔板。
  18. Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule

    其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。
  19. The exprossion of vascular endothelial growth factor and microvessel density in glioma

    血管內皮生長因子及其反義寡核苷酸對膠質瘤血管形成的影響
  20. Effect of et - 1 antisense oligodeoxynucleotide on the hemodynamics of normal and experimental hypertensive rats

    內皮素- 1前體基因反義寡核苷酸對正常和高血壓大鼠血流動力學的影響
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