接酶 的英文怎麼說

中文拼音 [jiē]
接酶 英文
ligase
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  1. We have identified in s. cerevisiae distinct ubiquitin - ligase complexes that define different erad pathways

    我們在酵母體內發現了一種參與不同erad途徑的特定的泛素連接酶復合體。
  2. Ligase an enzyme that catalyzes the bond formation between two substrates at the expense of the breakdown of atp or some other nucleotide triphosphate

    連接酶：可將兩個底物結合在一起的酶，此過程需要atp或其他核苷三磷酸供能。
  3. 4. the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi, and collected the digested cpti fragment and the pgem - 7z, then ligated by t4 dna ligase and formed the pgem - cp

    中間載體及表達載體的構建將puc - cp質粒和pgem ? 7z質粒，用kpni和bamhi酶切，分別回收cpti片斷和酶切后的載體片段，用t _ 4連接酶連接構建成中間載體pgem - cp 。
  4. Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

    Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。
  5. The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid

    將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。
  6. It does get into dna, but only when it expresses a particular enzyme called an integrase

    它確實會進入dna ，但只有當它表達一種特殊酶（稱為連接酶）時才行。
  7. The degree of bond formation by ligases is proportional to the amount of atp available in the cell at a particular instant

    連接酶合成產物的瞬時速度與細胞中可利用的atp含量成比例。
  8. In other cell types the gpi - linked enzymes can be released into the surrounding media by the appropriate triggers [ 42 ? 44 ]

    在其它細胞類型中， gpi連接酶通過適當的激發能被釋放到周圍介質中42 44 。
  9. Both rad6 and ubcls are ubiquitin - conjugating enzymes, and mms2 is a ubc - like protein. radls and rad5 are both single strand binding ring linger proteins. rad6 binds to radls and mms2 - ubcls binds to rad5

    在泛素系統中起主要作用的是三種酶：泛素激活酶e1 ；泛素綴合酶e2 / ubc和泛素連接酶e3 。
  10. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  11. To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully

    本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。
  12. Bacterium fluid is polluted or join is enzymatic give an issue did not hook up

    菌液污染或者連接酶出問題了沒連上。
  13. According to the mutliclone site of pcdnas plasmid, we have used xho i to cut pcdnas plasmid and make its linearized

    Pcdna3和doc一ir的酶切片段25經毛dna連接酶作用形成重組質粒。
  14. The same ability has been reported for some lymphocyte gpi - linked enzymes following lymphocyte actiation [ 43 ]

    某些淋巴細胞gpi連接酶隨著淋巴細胞的活化具有同樣的能力，這已經被報道過43 。
  15. 6 % respectively. 3. two sense plant expression vectors and two antisense vectors were constructed by fu

    Pbi121表達載體構建方法是酶切去除gus基因，利用t4連接酶代之以ginndl基因，構建成pzjll7和pzji18 。
  16. Wang sp, grayston jt. serotyping of chlamydial trachomatis by indirect fluorescent - antibody staining of inclusions in cell culture with monoclonal antibodies [ j ]. j clin microbiology, 1991, 29 : 1295

    余加林，吳仕孝.連接酶鏈反應在沙眼衣原體感染診斷中的應用[ j ] .重慶醫科大學學報， 1996 ， 21 : 409
  17. First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a

    用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。
  18. After digested with ecori and noti, the obtained dna fragment was linked with ppic9k by t4dna ligase and then the recombinant plasmid was transformed into competent dhscc. after positive transformants were sieved out by pcr, digesiting analysis and sequencing were also used to confirm the positive result more

    所得的dna片段經ecor和not雙酶切後用t _ 4dna連接酶與ppic9k載體進行連接，然後導入大腸桿菌dh5 ，用pcr法篩選陽性轉化子，並用雙酶切和序列測定方法鑒定重組質粒。
  19. To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis

    本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
  20. The construction of pci - il - 2 : primers were designed by the software oligo, xhol and sail were added to the primers, the cdna of il - 2 was obtained by pcr. the cdna of il - 2 was ligated with pci - neo cleaved by xhol and sail. the recombinant was evaluated by pcr

    Pcr法擴增幾億片段，在t4連接酶的作用下與xhol和sal工酶切的pci neo載體連接， pcr法鑒定重組體，用xho工， xhol sal ， ban工工酶切進一步驗證重組體，命名為pclll 2 。
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