核片段 的英文怎麼說

中文拼音 [hépiānduàn]
核片段 英文
nuclear fragmentation
  • 核 : 核構詞成分。
  • 片 : 片構詞成分。
  • 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
  • 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  3. The study results of this paper can serve the two scientific subjects of our teaching and research group as basic data calculation and elementary exploration. the two subjects are : constructing high activity - amylase genes by dna shuffling technology and studying on the evolution in vitro by mutational pcr ( with 5 - br - dutp as substitute partly ) and dna shuffling technology. - amylase ( ec 3. 2. 1. 1 ; 1, 4 - a - d - glucanohydrolase ) can catalyzes the hydrolysis of - 1, 4 - glycosidic bonds of starch from middle and liberates - maltose, - glucose and - limit dextrin stepwise

    本試驗根據genbank已公布的黃單胞菌-澱粉酶基因的核苷酸序列由引物設計軟體premierprimer5 . 0輔助設計了一對引物（ primer & primer ） ，以pbluescript ks +和puc18 / puc19質粒為載體，用常規的pcr方法從xanthomonascampestrispv . malvacearum （ smith ） dye等七株黃單胞菌（ xanthomomasspp . ）的基因組dna中克隆得到8個基因片段，分別命名為zhyf001 zhyf008 。
  4. Prokaryotic expression and characterization of n - terminal truncated glycoprotein gp85 of epstein - barr virus

    端片段的原核表達與初步鑒定
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software

    本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。
  7. 1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out

    具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。
  8. Dig labeled probe hybridization with solid pcr product was performed as well as electrophoresis of liquid product, this method combines rna purification, pcr amplification and nucleotide probe hybridization detection together and has many advantages including better rna purity, less time consumption, reliable positive reaction and 10 times sensitivity as rt - pcr gel running detection, reduce false positive result, unpurified nucleotide requirement, loug infected plant organism can be detected by solid hybridization

    2 ）結果可靠，雜交特異性誘捕目的片段，同時去除了核酸中存在的pcr抑制物質，減少了因核酸提取不純造成的漏檢現象，結果輸出通過雙重判定保證了結果的可靠性。 3 ）靈敏度高，通過雜交進行結果判定，靈敏度比傳統的rt干cr大約高10倍。
  9. Fachb440 was higher than that in the others. alignment based on deduced amino acid sequences indicated that spirulina fachb440 was different from that in other three samples of arthrospira. nucleotide sequence similarity among the three strains of the genus of arthrospira ( 96. 5 - 99. 6 % ) was higher than that between arthrospira and spirulina ( 78. 1 - 78. 5 % )

    Maximaouqdsm ）采螺旋藻（ spirulinafachb440 ） rubisco大亞基基因（ rbcl ）部分片段並進行了序列測定與分析，結果表明：螺旋藻（ spirulinafachb440 ）的gc含量比其他的節旋藻品系高；氨基酸序列分析發現螺旋藻與其他節旋藻品系的差異較大；螺旋藻與節旋藻核著酸序列相似性約為78
  10. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  11. By the analysis of blastn ( nucleitide level ) and blastp ( amino acid level ), some conversed domains in each protein sequence were dete cted, such as pas domain, pac domain and fhue

    對這4個片段進行核苷酸序列和蛋白序列的同源性比對，發現它們與一些功能保守的結構域具有同源性。
  12. The donkey - adapted strain of eiav ( dv116 ), parental strain of eiav - dla, is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s. the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period. in this study, we constructed a full - length provirus dna clone of dv116 by pcr

    本實驗中將eiav驢強毒dv體外感染驢白細胞培養物，一定時間后收獲病毒（本文中簡稱dv116 ） ，提取eiav前病毒dna ，以pcr法分別擴增並克隆了包含全長基因的三段前病毒dna片段，以雙脫氧法測定了dv116病毒全基因序列共8236個核苷酸。
  13. The cloning cdna fragment was extracted from positive clones and sequenced. the results showed that the cdna fragment was 816bp in size, encoding a protein which included 272 amino acids. the sequence homology analysis was carried out via the software blast 2. 0 network service in the four large databases - genbank, embl, ddbj, pdb, which had recorded 1 337 978 nucleotide and protein sequences. the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango, passion fruit, persia plum, strawberry, grape. . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 %. from the results mentioned above, we could confirm that the cdna of rubber tree etrl had been cloned

    從陽性克隆子中提取克隆片段，經序列測定分析，結果表明，克隆片段的cdna大小為816bp ，編碼的蛋白質包含272個氨基酸。基因序列通過blast2 . 0networkservice軟體對genbank ， embl ， ddbj ， pdb四個大型數據庫中記錄的1337978條核酸和蛋白質序列進行序列相似性檢索，結果表明與芒果、一西番蓮、波斯梅、草毒、葡萄、西洋梨等15種已報道的植物的etrl基因cdnag的同源率為75 88 ；蛋白質氨基酸序列的同源率為90 95 ，表明本研究確實克隆到了橡膠樹etri基因的cdna序列。 4
  14. In order to investigate the genomic organization of the single - nucleocapid nucleopolyhedrovirus of helicoverpa armigera, the ecori - n fragment located at 54. 8 - 59. 3 kbp of the viral genome was sequenced. the fragment contained 3762 bp helicase gene potentially encoding a protein with a molecular mass of 146 kda

    對棉鈴蟲單核衣殼核多角體病毒（ helicoverpaarmigerdsingle - nucleocapsidnucleopolyhedrovirus ， hasnpv ）基因組中ecori ? n片段進行序列分析，獲得了完整的解螺旋酶基因（ hel ） ，其開放閱讀框大小為3762bp ，編碼一個分子量為146kda的蛋白質。
  15. In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level

    本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆，通過核酸序列的差異比較分析，研究了該基因在不同物種中的進化關系；同時，通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究，獲得如下研究結果： （ 1 ）通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。
  16. Inherited genetic variation has a critical but as yet largely uncharacterized role in human disease. here we report a public database of common variation in the human genome : more than one million single nucleotide polymorphisms snps for which accurate and complete genotypes have been obtained in 269 dna samples from four populations, including ten 500 - kilobase regions in which essentially all information about common dna variation has been extracted

    我們現在發表一個人類基因組常見遺傳變異的公開資料庫：這是一個對來自四個群體269個樣品內的一百萬個單核苷酸多態性（ snps ）進行了完整並準確的分析的研究成果；另外也確認了十個dna片段（每個片段含有五十萬個堿基）的所有常見遺傳變異資訊。
  17. The 496 bp fragment of the orf of p22 gene and a 561 bp fragment were amplified from the genomic dna of zs strains of toxoplasma gondii. both 496 bp and 561 bp fragments were successfully cloned into the plasmid pthiohisa, b, c and pbudce 4. 1 respectively. 2

    從弓形蟲zs株基因組中擴增出p22編碼基因的一長496bp ，另一長561bp的片段，並成功構建含p22編碼基因的原核質粒重組體pthiohisa ， b ， c / p22 ，及真核重組表達質粒pbudce4 . 1 / p22 。
  18. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  19. This product fits for testing the even degree of one - metre - length ( 1 / 2m, 1 / 3m ) lap and the actual length of lap at the same time

    測定棉卷一米（或碼）長度片段的均勻情況，實際長度，核算棉卷的伸長率。
  20. Determine cotton roll - the uniform of meter ( yard ) sectional length and practical length. check and calculate the specific elongation of cotton roll

    測定棉卷一米（或碼）長度片段的均勻情況、實際長度，核算棉卷的伸長率。
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