核酶機制 的英文怎麼說
中文拼音 [héjīzhì]
核酶機制
英文
ribozyme mechanisms-
It is inferred that its active transcription occurs in the same region, not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rna polymeraes
這一結果不僅直觀地向人們表明了rna聚合酶在真核細胞核中的轉錄位點，而且對於人們進一步認識和理解rna聚合酶的轉錄機制、其轉錄產物的加工運輸途徑、以及真核細胞當中不同的rna聚合酶間的組織和調控關系都將有著重要的理論意義。From these results, it is inferred that the active transcription of pol iii occurs in the nucleoli and its periphery region, but not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase iii transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rnapolymeraes
本實驗為rna聚合酶在真核生物細胞核中的轉錄位點提供了較為直接的證據，這對人們進一步了解rna聚合酶的轉錄機制、加工和運輸過程及三種rna聚合酶之間的結構與功能關系具有重要的意義。Found the phenomenon observed in the nematode caenorhabditis elegans for the first time in 1998, consequently similar processes have been described for drosophila melanogaster, trypanosome, mammals including humans. the mechanism is that sirnas is the mediator, which can induce the risc to the target mrna and degrade it. recently there was great progress in the specific gene therapy and anti - virus, and rnai has been a focus of rna molecular therapy
自1998年fire等在研究線蟲時首次發現以來，相繼在果蠅、渦蟲、錐蟲、小鼠及哺乳動物細胞中發現rnai現象。一般認為： rnai第四軍醫大學碩士學位論文效應作用機制是sirnaskmallinterferinguaduplex ）作為中介分于，引導risc （ rnaiinducingsuppresscomplex ）至靶基因m洲a處，隨后核酸內切酶將之降解。Such examples are given as the blood filtering principal of hemoglobin, catalysis of enzymes, immune recoglization, prion, glycoprotein and the relationship of structure and function of membrane protein, et al, as well as the applications to medicine
在簡要介紹結構生物學的研究方法的基礎上，主要從分子水平闡述蛋白質和核酸的結構原理、相互作用、結構與功能的關系，通過具體實例闡述血紅蛋白的輸氧機制、酶的催化機制、免疫分子識別、朊病毒、糖蛋白、生物膜的結構功能關系等，以及結構生物學在醫學上的應用。Research interests : organic synthesis, including synthetic methodology, divergent oriented organic synthesis, asymmetric synthesis, asymmetric synthesis of small molecules with biological significance ( alkaloids, amino acids, pheromones, nucleosides analogues, enzyme inhibitors, receptor agonists and antagonists, bioactive heterocycles etc ) and chiral drugs
研究興趣：主要從事有機合成化學及相關的化學生物學研究，包括合成方法學、多樣性導向有機合成、不對稱合成、有機小分子調節劑（生物堿，氨基酸，昆蟲信息素，酶抑制劑，受體拮抗劑，核苷類似物等）和手性藥物的不對稱合成。A brief introduction to the x - ray crystallographic studies on rna polymerase complexes and the enzymatic mechanisms revealed by the crystal structures
摘要用x射線晶體學方法測定的一系列rna聚合酶復合物結構揭示了真核轉錄的分子機制。Its km for sacilin at50 andph5. owas3. 73mg / ml. with the analysis of kinetics, the p - glucosidase showed synergistic action to the endoglucanase and the endoglucanase inhibited the p - glucosidase. the mechanism for their interaction was explained that the endoglucanase was combined with its products and the inhibition of these products to endoglucanase was removed by its hydrolysis by p - glucosidase
經兩組分共同作用的酶反應動力學分析后發現，兩組分這種相互作用機制的核心是：內切酶和?葡萄糖苷酶與內切酶的水解產物，也是其底物類似物? ?纖維二糖和纖維寡糖的結合以及-葡萄糖苷酶對該產物的水解。This review focuses on morphological and physiological reactions of fruit tree to water stress. leaf area, root growth and microstructure of leaves and roots were investigated. some physiological and biochemical index of fruit tree leaves and roots under water stress, such as variations of stomatal aperture, photosynthesis, photoinhibition, metabolism of lipoxygenase, content of proline, nuclear acid and endogenous phytohormones, were summarized
從水分脅迫對果樹葉、根的形態指標及顯微結構，葉片氣孔行為、光合作用、光抑制、活性氧代謝、脂氧合酶代謝、多胺代謝、脯氨酸、核酸代謝、內源激素變化等生理生化方面綜述了近十幾年來的研究成果，為全面研究果樹抗旱機理及進一步制定抗旱措施奠定理論基礎。Kinetic study shows that nadp + is the only valid coenzyme and that one substrate have no effect on the the other substrates " bind with the enzyme. products are competitive inhibitors, nadp + / nadph and 6 - phosphogluconate / ribulose - 5 - phosphate pairs are competitive whatever the concentration of the other substrates but noncompetitive versus the other substrates. therefore the kinetic mechanism of the 6 - phosphogluconate dehydrogenase is haldance - laidler - socquet random mechanism
兩種底物與酶的結合互不影響，而在產物抑制效果上， 5一磷酸核酮糖只競爭性抑制6一磷酸葡萄糖酸， nadph只競爭性抑制nadp + ，所以該酶催化機理是haidance一laidier一socquet隨機機理。It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified
中國豬瘟兔化毒（脾淋毒）基因組cdna文庫的構建、序列分析：根據已發表的豬瘟病毒（ csfv ）核苷酸序列，藉助計算機軟體分析，選擇高保守區段和基因組中的單一限制性酶切位點，利用rt - pcr及nested - pcr和helf - nestedpcr技術，成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ，分別克隆到pmd - 18t或pgem - teasy載體進行測序后，拼接出了其核苷酸序列。The last seven years have spawned a vast amount of data on the enzymes that remodel and modify nucleosomes and the rules governing how transcription factors interact with the epigenetic code on histones
在過去的7年中，科學研究提供了大量的有關以下兩個方面的數據： （ 1 ）具有重構和修飾核小體功能的酶，以及（ 2 ）轉錄因子如何同組蛋白上的表觀遺傳編碼相互作用的機制。And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。A eukaryotic expression vector pcdna3. 1 - cptl was constructed by insert cp77 gene into the vector pcdna3. 1 which is used in nucleic acid immunization. the vector was immuned the balb / c mice by the method intramuscular injection after extracted and purified in great deal. immu - nological reaction was induced by the expression of cptl after the vector entered into the mice body
本研究通過限制酶將cpti基因片段從載體pbluel3上切下，插入真核表達載體pcdna3 . 1 ，構建了用於核酸免疫的真核表達載體pcdna3 . 1 - cpti ；質粒大量提取和純化后，通過肌肉注射的方法免疫balb c小鼠，基因表達產物刺激小鼠機體產生免疫反應，從而獲得了抗cpti蛋白的抗體。分享友人