核酶 的英文怎麼說
中文拼音 [hé]-
Research on changes of the nitric oxide synthase - positive neurons in medial cortical amygdaloid nuclei of rats under stress of exercise - induced fatigue
疲勞應激大鼠杏仁皮質內側核群一氧化氮合酶陽性神經元的變化It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證As the cyclin dependent kinase, cdc2 may act at multiple levels during mitosis to repress ribosome biogenesis, which lead the biosynthesis to a relative silent phase, when the most of cell ' s energy expenditure is used in chromosome condensation, breakdown of the nuclear envelope, and formation of the mitotic spindle
作為cyclin依賴激酶， cdc2的激酶活性在很大程度上抑制了細胞的合成代謝，使細胞的蛋白合成進入了一個相對的靜止期，而細胞主要的能量則被應用於細胞分裂時的染色體的濃縮、核膜的降解、紡錘體的形成等。In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation
本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。Constructing human colorectal cancer cell line with stably down - regulated grp94 ( 1 ) the plasmid prc / rsv - ribol that contains specific grp94 - targeting ribozyme and the control plasmid prc / rsv were miniprepared, respectively, cleaved by endoenzyme pvuii
穩定下調grp94的人大腸癌細胞克隆株的構建（ 1 ）分別對含有特異性打靶grp94核酶的質粒prc rsv - ribo1和對照組質粒prc rsv進行小量提取、 pvu酶切鑒定。Ribozyme structural elements : hairpin ribozyme
核酶結構元件Ribozyme structural elements : group i introns
核酶結構元件A specific cis - hairpin ribozyme facilitates the infection in tobacco protoplasts
一個特殊的順式發夾核酶對感染煙草原生質體的影響Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表達載體的構建及對番茄的轉化As indicated above, several m1gs that cleaved hcmv ul54 mrna segments in vitro were successfully designed and constructed. our studies demonstrates the utility of this ribozyme m1gs for antiviral application
我們的研究成功地利用引導序列，將核酶rnasep催化亞單位m1rna構建為序列識別的核酶m1gs ，證實了核酶在抗病毒方面的應用價值。Sequencing report of pcr product shows the ribozyme gene with two cleavage sites has already integrated into the genome of potato. and it also proved 35s promoter of prok2 was same as that of hajdukiewicz, p. and rna detection is going on
檢測結果證明：所擴增的dna包含核酶基因反轉錄后的dna ，證明核酶基因已被轉入馬鈴薯j - 1中；同時證明了轉基因的prok2的35s啟動子與hajdukiewicz ， p等所公布的啟動子相同。Based on this consideration, we chose human colorectal cancer cell line ccl229 as the experimental object, down - regulating its grp94 expression by specific ribozyme targeting. we observed its effects on the biological characteristics of ccl229 and upr pathway, and gained further understand of the grp94, upr and tumor relationship
基於此，我們選擇了人大腸癌細胞株ccl229作為研究對象，通過特異性核酶打靶的方式，下調細胞中grp94的表達水平，觀察其對人大腸癌細胞某些生物學特性及upr通路的影響，加深對grp94 、 upr和腫瘤這三者間關系的了解。Different migs / substrate reacting concentrations were tested, the best migs / substrate ratio was 2 : 1 and too much m1gs led to substrate degrading. it was also found that the secondary structure affected the action of m1gs
對m1gsdna模板和4個底物模板分別進行體外轉錄，獲得核酶m1gs的rna片段以及帶有~ （ 32 ） p放射性標記的四個底物rna片段。Inhibition of hyperplastic scar with ribozymes target in type and pre - collagen
型前膠原基因核酶抑制增生性瘢痕的實驗Intervention of ribozymes of type and procollagen on hyperplastic scar of nude mice
型前膠原核酶對裸鼠增生性瘢痕的干預Ribozymes and evolution
核酶和進化M1gs can cleave any rna segments in vitro when the rna is in a complex with its guide sequence ( gs )
附帶gs的m1rna成為了具備自我引導和序列特異切割能力的核酶? m1gs 。Association of dna enzyme targeting egr - 1 with the expression of inducible nitric oxide synthase in rats after balloon injury
生長反應因子1特異的脫氧核酶與大鼠頸動脈損傷后誘生型一氧化氮合酶表達的關系The detection of ribozyme gene with two cleavage sites that cleaves plrv replicase gene were present in the second part of this paper. a conserved sequence of 35s promoter of plrv was found in gene pools. two primers were designed based on the conserved sequence and bamh i and ribozyme gene. the genomic dna of potato was amplified by the primers through polymerase chain reaction ( pcr )
從許多資料中報道的plrv的35s啟動子的序列中找出一段保守序列，然後根據與核酶緊密相連的bamh和這段保守序列設計兩段引物，用這兩段引物通過pcr擴增轉基因馬鈴薯的基因組dna ，並進行檢測。The recombinated plasmid was named pu54. based on this material, 4 recombinated plasmids pu54 - a, pu54 - b, pu54 - c and pu54 - d were construted, which contained different cloning segments of ul54 gene. 11 m1gs ribozyme containing different gss designed to the target sequences of ul54 mrna were construted by pcr
從hcmvdna聚合酶ul54基因序列中搜尋了11個適合gs互補的靶位，人工核酶m1gs轉錄模板dna包含有t7啟動子、 m1rna基因、連接序列以及gs序列四個組成部分。分享友人