核酸探計 的英文怎麼說
中文拼音 [hésuāntànjì]
核酸探計
英文
nucleic acid probe-
Prepare the oligonucleotide probes designed 16 probes as four groups according to hla - dqa1 sequence in genbank and modify each probes by ammonia at 5 ' - end. then resuspended the lyophilized probes in ph = 9, 0
寡核苷酸探針的制備根據genbank數據庫新近公布的hla - dqa1等位基因序列，設計四組共16條特異性寡核苷酸分型探針，並在各5 』端做氨基修飾。We designed a suitable probe in the experiment and used ribonuclease protection assay technology to detect the expression of pdipl gene in mouse liver at different time points after partial hepatectomy. we found that the expression of pdipl gene was increased. it reached the highest level after 12 hours and then decreased
我們設計合適的探針，採用核酸酶保護反應等技術測定了pdip1 -基因在肝臟部分切除后不同時間在小鼠肝臟中的表達，發現pdip1 -基因在肝臟部分切除后表達增強， 12小時表達最強，以後表達減弱。The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1, respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus, which had no analogical to human gene. mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell
第三章，根據一種常見的病毒煙草花葉病毒（ tmv ）的核酸序列設計了分子信標熒光探針，由於tmv的遺傳物質是rna ，分子信標又具有很高的特異性和靈敏度，因此感染了病毒粒子的植物葉片在經過簡單處理后，可用分子信標檢測葉片上。With the rapid development of molecular biology, the researchers of different scientific background are provided with a good opportunity to enter the field. people can resolve some important difficult problems with all kinds of research methods and knowledge in their fields. it is chemists " tribute that they design and synthesize effective nucleic acid cleavage reagents and clarify the reaction mechanism of complexes and dna, which makes it possible to search effective remedial reagents and structural probes by molecular design
分子生物學的迅猛發展為不同科學背景的研究者涉足該領域提供了良好的機遇，人們可以利用各自領域的研究方法和知識來攻克生物學中的一些重要難題，化學家所能做的貢獻就是設計和合成一些特異識別和高效切割的核酸斷裂試劑，並闡明其作用機理，從而使通過分子設計尋找有效的治療試劑和結構探針成為可能。The massive amount of high - throughput microarray, snps and other biological data bring a great challenge of developing advanced statistical and computational data mining tools
大量的高產能微陣列、單點核苷酸多型性及其他生物資料也對高級統計計算之資料探勘工具產生極大的挑戰。The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced
首先以中肋骨條藻的rdna序列為設計種特異性引物的靶區域，共設計出7對適合rfq - pcr的引物（依次命名為primer1 （ f r ） primer7 （ f r ） ） ，並用常規pcr實驗初步證明其中有4對引物（ primer1 （ f r ） 、 primer2 （ f r ） 、 primer5 （ f r ） 、 primer6 （ f r ） ）可作為中肋骨條藻特異性引物的候選者；然後測定了以primer6 （ f r ）為引物的pcr產物的序列，序列分析表明，中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大，從中可設計出滿足rfq - pcr需要的taqman探針（命名為taqman6 ） ；進一步的核酸雜交實驗表明， taqman6隻與中肋骨條藻的pcr產物雜交，不與其他藻的pcr產物雜交。There are four imperfect repeats v - v - e - k - k - n / e - e of which the core sequence is similar to map ib of mouse. rna blot and rt - pcr analysis showed that this gene is expressed specifically in the mature pollen and can be classified as a late gene in pollen development
計算機軟體分析st901基因核苷酸、氨基酸序列的相似性，結果表明， st901基因編碼區與探針sb401 、與高賴氨酸基因sblr 、與番茄tsb具有較高的相似性，它們可能來源於馬鈴薯的一個基因家族。Calculate the distibution of the melting temperature of the oligonucleotide probe sets that affymetrix uses for its microarrays
計算用於微陣列的基因表達譜、和基因分型研究技術平臺使用的、低聚核苷酸探針裝置的溶解溫度分佈。This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv
為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently. lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques, such as immunological screening, drug - prob screening, southern et al. lt is very important to study the life nature of plasmodium falciparum in molecular level. with the developments of these studies, the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known. at the same time, the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs
建立cdna表達文庫在一次永久保存基因資源的同時，可以利用功能篩選、免疫學篩選、藥物探針篩選、 southern雜交和大規模序列測定等現代分子生物學技術尋找特異性活性蛋白基因，進而克隆和表達這些基因，對從核酸及蛋白質等分子水平研究瘧原蟲的生命活動規律，對揭示其抗藥性分子機理，搞清某些特效藥物結合蛋白的基因及此類藥物的作用機制，對新型抗瘧藥物的合理設計及篩選都具有極其重要的現實意義。To explore the feasibility of ib edible vaccine, we have transformed si gene of ibv into potato and researched the immunogenicity of it ' s expression product. the findings are as follows : 1. a pair of primers for ibv si gene were designed and synthesized according to the published nucleotide sequence of ibv si gene and multiclone sites of expression vector pbi121
為了探索ibv可食性疫苗的可行性，我們進行了轉基因馬鈴薯表達ibv免疫原基因及其表達產物免疫原性的研究，取得以下結果： 1根據周繼勇等報道的ibv - zj971毒株s1基因核苷酸序列和pbi121植物表達載體的多克隆位點，設計併合成引物，以含s1pbs質粒為模板擴增s1基因，將擴增片段定向克隆到pbi121質粒的35s啟動子下游。分享友人