核酸探針 的英文怎麼說

中文拼音 [hésuāntànzhēn]
核酸探針 英文
nucleic-acid probe
  • 核 : 核構詞成分。
  • 酸 : 酸構詞成分。
  • 探 : Ⅰ動詞1 （試圖發現） try to find out; explore; sound 2 （看望） call on; visit; see 3 （向前伸出）...
  • 針 : Ⅰ名詞1 （縫衣物用的工具） needle 2 （細長像針的東西） needle like things 3 （針劑） injection; sh...
  • 核酸 : [生物化學] nuclein; nucleic acid核酸聚合酶 nucleic acid polymerase; 核酸酶 nuclease; 核酸內切酶 [生物化學] endonuclease
  • 探針 : probe; sound; filling fork; feeler; explorer; probing pin; touch needle; wire probe
  1. Prepare the oligonucleotide probes designed 16 probes as four groups according to hla - dqa1 sequence in genbank and modify each probes by ammonia at 5 ' - end. then resuspended the lyophilized probes in ph = 9, 0

    寡核苷酸探針的制備根據genbank數據庫新近公布的hla - dqa1等位基因序列，設計四組共16條特異性寡核苷酸分型探針，並在各5 』端做氨基修飾。
  2. In this study, the meaningful results have been achieved, which is the important basic work to research the pili subunit vaccine of avian e. coli, and detect pila gene by nucleric acid probe. moreover, it is significant to research molecular epidemiology, to diagnose, to prevent and treat avian colibacillosis

    本研究為雞源致病性大腸桿菌菌毛基因工程疫苗的研究，制備核酸探針檢測pila基因提供了重要材料，對雞大腸桿菌病的分子流行病學研究、診斷和防治研究具有重要意義。
  3. Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers

    嘗試應用多位點dna指紋技術，檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明：採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜，具有足夠的多態性和很好的穩定性。
  4. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  5. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。
  6. Was multiplied and the tk gene was cloned. the cloned tk gene was retrieved by proper restrictive hemodynamics. the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin. then, the specificity and sensitivity of tk gene probe were detected with dot blot hybridization. the sequence of tk gene of nm98a strain was analysed. the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99. 7 %

    本研究中首次對iltv - nm98a株的tk基因進行了克隆和序列分析，結果表明： iltv - nm98a株tk基因的核苷酸序列與已發表的iltvtk基因的核苷酸序列具有高度的同源性，兩者之間僅相差4個核苷酸，同源性高達99 . 7 ，從而證實了iltvtk基因是高度保守的，為iltvtk基因核酸探針的制備提供了有力的依據。
  7. Calculate the distibution of the melting temperature of the oligonucleotide probe sets that affymetrix uses for its microarrays

    計算用於微陣列的基因表達譜、和基因分型研究技術平臺使用的、低聚核苷酸探針裝置的溶解溫度分佈。
  8. The results of the experiments indicates that concentration of aminosilane influences the fluorescence background of glass slide, and some factors affect immobile ratio of oligonucleotide probe, such as aminosilane treatment time, aldehyde treatment time, uv crosslinking energy, washing temperature and time

    研究表明，氨基化試劑濃度對玻片熒光背景有影響，氨基化試劑處理時間、醛基化處理時間、紫外交聯能量和洗滌溫度和時間等工藝因素影響寡核苷酸探針的固定率。
  9. The key stage of fabricating gene chip is pretreatment of glass surface including the processes of nh3h2o treatment, aminosilane treatment and aldehyde treatment. the pretreatment can grow active group that can bind probe effectively on the surface of glass slide. as a result, the actively treated glass slide can suit for fabricating in - situ synthesis high density gene chips

    基因晶元制備技術的關鍵步驟是玻片表面預處理，即對玻片表面進行羥基化、氨基化和醛基化處理，使表面生長的活性基團能有效固定寡核苷酸探針，以滿足原位合成高密度基因晶元對玻片的要求。
  10. First, glass slides having been rinsed will be treated with nh3h2o, aminosilane and aldehyde. second, the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope. in the end, the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods

    實驗選用表面平整的德國玻片，將清洗好的玻片分別進行羥基化、氨基化、醛基化，採用熒光法和原子力顯微鏡法分別檢測玻片表面預處理質量，研究兩種檢測方法之間的內在聯系，從而確定表徵玻片表面寡核苷酸探針固定率的方法。
  11. The effects of glp - 1 ( 7 - 36 ) nh2 on insulin secretion glp - 1 ( 7 - 36 ) nh2 with the concentration of 2. 5nmol / l, 5. 0nmol / l, l0. 0nmol / l, 20. 0nmol / l, 40. 0nmol / l respectively were added to the medium as different experimental groups, 24 hours later, insulin amount are 68. 76 ? 1. 71 72. 30 ? 3. 13 104. 16 ? 5. 57 110. 98 ?. 29 111. 58 ? 0. 65miu / l respectively, and the insulin account is 55. 53 ?. 63miu / lin the control group. there was no significant difference between the groups with 2. 5nmol / land 5. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively ; and there was not significant difference among the groups with lo. onmol / l, 20. 0nmol / l and 40. 0nmol / l glp - 1 ( 7 - 36 ) nh2 respectively. but the difference is significant between experimental groups and control group ( p < 0. 05 ). the data show that with the rising concentration of glp - 1 ( 7 - 36 ) nh2, there is an increasing amount of insulin

    對照組培養液中不含g廿一1 （ 7一36 ） nhz ，實驗組培養液中含有20nmol / lglp一1 （ 7一36 ） nhz ，培養24h后，用0 . 25 %胰蛋白酶消化胰島分散細胞，塗片后利用針對胰島素mrna的寡核甘酸探針進行細胞原位雜交， dab顯色，高清晰度病理圖文分析系統（ highpathologiealimageanalysissystem ， hp認s ）對細胞著色的平均光密度（ mean即tiealdensity ， mod ）量化分析，觀察實驗組和對照組胰島素mrna的表達情況。
  12. The author reviewed the detection measures of prunus necrotic ringspot virus, and related the research progression of the pathogen detection technology inside and outside. the template amplication technology include pcr assays ^ nasba and so on. the cdna and crna probe which labeled with the isotope % biotin or dig, the offset probe and the peptide probe can be applied to magnify the signal. pcr - gene scan assays and pcr agilent chip chamber combine the template amplication and the signal magnification

    本文回顧了李壞死環斑病毒的檢測方法，較全面地評述了國內外病原物檢測技術研究進展：在模板的擴增有各種pcr技術、 nasba技術；在信號的放大有同位素、生物素或dig標記的cdna和crna探針，分支探針和肽核酸探針；模板擴增和信號放大相結合的有pcr -基因掃描技術、 pcr安捷倫晶元實驗室技術；模板擴增和雜交以及信號放大相結合的有pcr - elisa技術、實時熒光pcr技術、生物晶元技術。
  13. The result of fluorescence show that the fluorescence intensity of the surface of the treated glass slide connect with the probe immobile ratio of oligonucleotide. the more oligonucleotide probes have been linked with active group, the stronger fluorescence intensity is. for the strongest fluorescence, the technical conditions is : treatment of 2 % aminosilane of 20 minutes, treatment of 5 % aldehyde of 24 minutes, uv crosslinking of 150mj and washing of 5 minutes at 20

    兩種檢測方法表明，當活性基團呈柱狀、分佈均勻且尺寸比較大（ 200nm ）時，有利於寡核苷酸探針的連接，且連接探針數量多，玻片表面熒光強度強，固定率高；當活性基團呈錐狀、分佈及尺寸不均勻（ 150nm （ 300nm ）時，連接的寡核苷酸探針數量少，玻片表面熒光強度弱，固定率低。
  14. 5. prepare the oligonucleotide microarray add 16 prepared probes into 384 - pole plate, meantime, add p2, low homologic probes and probe buffer in the same concentration as positive control, blank control and negative control respectively. spot microarray as designed by the spotting machine

    5 .寡核昔酸微陣列的制備以同等濃度的反義鏈引物咫作為陽性對照，以探針緩沖液作為空白對照，以無關探針作為陰性對照，與制備好的16條寡核昔酸探針，同時加人384孔板。
  15. Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule

    其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。
  16. Nonradioactive nucleic probe to tgev was prepared and was applicated to detect tgev at first stage, which established foundational work for differentiating tgev from prcv and investigation on epidemiology of tgev

    制備了tgev非放射性標記的核酸探針，並初步應用於檢測tgev 。二者為tgev鑒別診斷、血清學調查奠定了基礎。
  17. The specificity of the nucleic acid probe was very strict. lt reacted positively with iltv dna only and it react negatively with the nuleic acid of ndv, bv and ibdv. the sensitivity of this kind of probe is very high. ilt could even detect 20pg ' s iltv dna

    結果表明：該種核酸探針具有高度的特異性，它僅與iltv的dna呈現陽性反應，而與新城疫病毒、傳染性法氏囊病病毒和傳染性支氣管炎病毒的核酸等均呈陰性反應。該種探針具有高度的敏感性，能夠檢測到20pg的iltv的dna 。
  18. 2. the optimize of the oligonucleotide probes preparation the optimal condition is to resuspend the probes in ph = 9, 0. 1 m carbonate buffer to 100 m. 3

    2 .寡核昔酸探針制備條件的優化以ph二9 ， 0 . im的碳酸鹽緩沖液，重懸為100林m探針為最優條件。
  19. Microarray of single stranded deoxyribonuleic acid probes on the surface of zro2 ceramic beads and the preparation of fluorescent ceramic beads

    單鏈脫氧核糖核酸探針在氧化鋯陶瓷小珠表面的微陣列及陶瓷熒光小珠的制備
  20. These results show that cdna expression library has an excellent quality and lays solid foundation for further screening. in addition, the library is also used for screening other breast cancer relevant genes

    同時由於該文庫包含了此乳腺癌細胞系的全部基因信息，因此也可以用其他的抗體或寡核昔酸探針篩選乳腺癌的相關基因。
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