液化蛋白 的英文怎麼說

中文拼音 [huàdànbái]
液化蛋白 英文
liquefied protein
  • : 名詞(液體) liquid; fluid; juice
  • : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
  • : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
  • 液化 : [化學] liquefaction; deliquesce; liquefying; liquidation液化劑 liquefier; fluidifiant; liquefacie...
  • 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
  1. After the expression form analysis, the insoluble recombinant proteins was purified by destraction and abstersion of inclusion bodies. to study the abstersion condition of the inclusion bodies, we adopted ultrasound crushing and freezing - melting methods

    採用超聲加洗滌破碎菌體;離心加凍融分離純融合,研究不同的超聲次數和凍融對包涵體洗滌效果的影響。
  2. Other non - peroxidated aldehydes, such as formaldehyde, acetaldehyde, glyoxal, acrolein, pyruvaldehyde, salicylaldehyde, all did not change rheological property of these biological materials. the effects of mda on rheological parameters, crosslinking reaction and their relation with age pigment - like fluorescence were studied

    戊二醛作為一種固定劑能特異地改變流體特性,而其它非脂質過氧醛類,如甲醛、乙醛、乙二醛、丙烯醛、丙酮醛、水楊醛等並沒有明顯改變上述溶的流變性。
  3. Study on treatment of soybean albumen whey wastewater using pre - oxidation - coagulating sedimentation method

    預氧混凝處理大豆乳清廢技術探討
  4. The pancreatic juice also contains three main enzymes which tear proteins, fats and carbohydrates apart into basic building blocks.

    內還含有三種主要的酶,這些酶能把質、脂肪和碳水合物分解成基礎建築材料。
  5. The antibody of ceruloplasmin is modified on the qcm by the approach above and the determination of ceruloplasmin is carried out in the buffer solution with 3. 5 % polyethylene glycol ( peg )

    採用上述固定方法,將銅藍抗體吸附固定於石英晶體表面,在含有3 5的聚乙二醇( peg )緩沖溶中測定人血清銅藍(抗原) 。
  6. The results showed that the cucumber seeds soaked with extracts of total alkaloid, dissoluble alkaloid and fat - soluble alkaloid from p. multisectum ( maxim. ) bobr., the activities of amylase, protease and lipase during seed germination were inhibited, the seed vigor and germination rate were suppressed, and respiration rate of seedling was slackened ; root activity, chlorophyll content and activities of nitrate reductase, superoxide dismutase sod ) and peroxidase ( pod ) of cucumber seedlings during seedlings growth were increased

    結果表明,多裂駱駝蓬總生物堿提取、水溶性生物堿提取和脂溶性生物堿提取浸種均抑制黃瓜種子萌發過程中澱粉酶、酶和脂肪酶活性,種子活力和萌發速率降低,呼吸速率減慢;幼苗生長過程中根系活力、硝酸還原酶活性升高,葉綠素含量增加,超氧物歧酶( sod )和過氧物酶( pod )活性提高。
  7. Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4

    Sds page分析得到3條主要帶,剪下這三條帶進行膠內賴氨酸內切酶的消,通過高效相色譜分離肽段,選擇性進行肽段的氨基酸序列測定。
  8. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中,轉大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總量的26以上。
  9. Expanded bed adsorption ( eba ) is a novel bioseparation technique, which integrates clarification, concentration and initial purification into a single unit operation. it enables proteins to be recovered directly from unclarified cultivations of microorganisms or cells and homogenates of disrupted cells, without the need for prior removal of suspended solids. matrix is the principal " hardware " pillar supporting the successful application of eba

    擴張床吸附( eba )技術是一種新型的生分離技術,它集成了固分離、濃縮和初期純於一步單元操作之中,可以直接從含有細胞和細胞碎片的發酵或培養中提取目標,而不必事先除去懸浮的固體顆粒。
  10. Optimization technology of preparing the solution of wool keratin with method of reduction - c

    法制備羊毛角質溶的工藝優
  11. According to the physical - chemical property of the wool and the actions of the chemilacs on the wool in the solution, we take all kinds of measures such as bleaching, osmosis, setting, etc, and combine with the experiments data of the wool under the different chmical conditions. therefore, we have found the best way of the wools chemical mercerization

    根據羊毛質纖維的理性質及其在水溶中與學藥劑發生作用的情況,我們採取了各種漂、滲透、定形等措施,並結合羊毛在不同學條件下絲光情況的實驗數據,得出最佳學絲光羊毛的工藝路線。
  12. The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e. coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method. several conditions, which include diffend ph buffer and concelltheion of imidazole, were selected to purify recombinan nucleorotein

    根據載體pproexhtb含有( his ) 6特點,將融合進行純,在純過程中經各項條件的探索,確定為在裂解中含有1mmpmsf的條件下,分別經過2倍體積的buffera和bufferb洗脫后,再收集ph5 . 9 ,含有80mmol / l咪唑的1倍體積bufferc洗脫,可得到純的融合
  13. With the increase of concentration of aqueous extract from peganum multisectum increased, root vigor, the contents of chlorophyll, soluble protein and nucleic acid in roots and shoots of alfalfa seedlings decreased, while the activities of protease and nuclease, the contents of o2 ( superscript - ), h2o2 and malondiadehyde ( mda ) increased, the activities of superoxide dismutase ( sod ), catalase ( cat ) and peroxidase ( pod ) first increased and then decreased

    幼苗根系活力和葉綠素、可溶性質、核酸含量隨水浸濃度的提高而降低,酶和核糖核酸酶活性及超氧陰離子( o2 (上標- ) ) 、 h2o2和丙二醛( mda )含量則增加,超氧物歧酶( sod ) 、過氧氫酶( cat )和過氧物酶( pod )活性呈先升后降變
  14. The protein inclusions in a granular appearance accumulated in the central vacuole and starch grains generally in the cytoplasm of ordinary parenchyma cells

    免疫熒光和電鏡免疫細胞學定位證明,它們是貯藏質細胞的質內含物的主要成分。
  15. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株體發酵,通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸酶,生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用,同時測定了該酶理特性,酶活力在75附近酶活力最高,隨著ph的增加酶的穩定性升高,與膽堿酯酶具有相似的ph曲線,對特異性底物aape ( suc - ala - ala - pro - glu - pna )具有作用, ssi和ci - 2抑制該酶的活性。
  16. It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation

    最佳氮源為玉米漿和腖,最佳比例為2 : 3 ,最佳濃度為1 . 6 ;加入其它碳源時有助於菌株穩定產酶; 100ml搖瓶的最佳裝量為15ml ;肌酸、肌氨酸和氯膽堿都能誘導菌株產酶,其中肌酸誘導產酶的效果最好,而肌酐和尿素不能誘導菌株產酶;誘導物肌酸的最適加入時間為接種培養12小時后,最適加入量為0 . 75 。
  17. Salt treatment had effects on growth, succulence and some physiological parameters. in present study, suaeda salsa seedlings were treated with different salts and isoosmotic peg to examine the succulence and some physiological parameters. the hydraulic conductance ( lo ) of the roots, the water permeability of protoplasts and western blot analysis of aquaporins in plasma membrane and tonoplast under nacl were determined

    本實驗以鹽生植物堿蓬幼苗為材料,用不同的鹽及與nacl等滲的peg處理,測定肉質及有關生理指標,並測定nacl處理下植物根的導水性,原生質體的水滲透性,並在分子水平上進行了細胞質膜及泡膜水孔免疫雜交分析。
  18. Na + / h + antiporter is one sort of protein which on the membrane or vacuole membrane, they can transport na + retrorsely out of the cell or into the vacuole. most of the algaes and the salt plants have this kind of protein

    Na ~ + h ~ +運輸是位於質膜和泡上的一類運輸,它可以逆電學梯度將na ~ +排出細胞外或區隔泡中,普遍存在於藻類與鹽生植物中。
  19. Change of the proteoglycan and hyaluronic acid in different symptom type of knee oa in human synovial filud

    膝骨性關節炎不同中醫證型滑多糖和透明質酸含量的變
  20. Method to collect respectively 180 unrelated males " venous blood 500ul, who lived in shanxi province, 120 unrelated mongolians " venous blood 500ul, who lived in the inner mongolia autonomous region, and the blood is anticoagulant with edta, then to extract dna by using the method of phenol - chloroform after ingested by proteinase k at 56 and amplify the dys413 site by using pcr

    方法採集180例山西漢族和120例內蒙古蒙古族男性無關個體靜脈血各500ul , edta抗凝,用tkml反復洗滌至無色,加入2酶k緩沖180ul ,酶k ( 20mg ml ) 20ul ,在56消體清亮為止,用酚-氯仿法抽提dna , pcr擴增dys413位點, 6非變性聚丙烯酰胺凝膠電泳, 1硝酸銀染色分型。
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