溶酶體基質 的英文怎麼說
中文拼音 [róngtǐjīzhí]
溶酶體基質
英文
matrix of lysosome-
Abstract : numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
文摘:許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料Numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease
許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料The obtained biosensor exhibits high sensitivity, excellent reproductivity and good stability with substantially improved performance. part two describes the manufacture and characterization of glucose oxidase - silver sol - polyvinyl butyral modified platinum electrodes with tris ( 2, 2 ' - bipyridyl ) cobalt ( iii ) perchlorate as an electron transfer mediator in the glucose solution
用納米ag溶膠固定god ，採用聚乙烯醇縮丁醛為輔助固酶膜基質修飾鉑絲電極，並以葡萄糖溶液中的co恤pyh （ cio4 ） 3為電子媒介體組成葡萄糖氧化酶生物傳感器。Nucleoli do not emerge in this process. the number of the organelles increase until secondary spermatocyte stage. mitochondria accumulate together, merging together with lysosomes and golgi bodies at the early spermatid stage, and finally the lamellar structure is formed, which forms the acrosome at last
在精子發生過程中，線粒體、內質網和核糖體逐漸增多，其中線粒體數目在次級精母細胞階段達到頂峰，並形成線粒體區，精細胞早期核內出現膜性泡結構，同時次級溶酶體與高爾基體大量存在，這些細胞器共同形成片層復合體，並參與頂體的形成。The number of mitochondrion is more less than the endoplasmic reticulum, and the smooth endoplasmic reticulum is the main kind of the endoplasmic reticulum ; golgi bodies and lysosomes emerge in the secondary spermatocyte stage. finally, these organelles change into pre - acrosome vesicles which become acrosome at last. sinopotamon chekiangense during the spermatogenensis, chronmatins condense at different level until middle spermatid stage
在整個發生過程中細胞器數量較少，內質網數目在各細胞器中所佔比例最大，以滑面內質網為主，線粒體在初級精母細胞中最多，自次級精母細胞開始逐漸減少，高爾基體和溶酶體自次級精母細胞始出現，在發育過程中上述細胞器不斷分化，在精細胞階段形成前頂體腔，最後形成圓球形頂體。First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后，考察了影響兩親株原生質體形成和再生的主要因素，確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件：前者用s培養基，在28 、 220r . min ~ （ - 1 ）培養24h后，用3mg ml的溶菌酶在32恆溫酶解50 60min ，得到的原生質體在乾燥的r5培養基上28倒置培養5 6天，可得到再生率在20左右的再生菌落；後者採用二級菌絲培養，用1mg ml的溶菌酶在37恆溫酶解1h左右，得到的原生質體也在乾燥的r5培養基上28倒置培養5天，即可得到再生率在20左右的再生菌。A novel aqueous two - phase system can be formed by the mixtures of a polymer and cationicanionic surfactants. such a system can be used as a partitioning system of proteins. in this work, we investigated the formation, phase behavior and protein partitioning in aqueous two - phase systems formed by dodecyltriethylammonium bromide / sodium dodecylsulfate / peg and dodecyltriethylammonium bromide / sodium dodecylsulfate / dextran. the ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. it was shown that the surfactants and polymers are enriched in different phases of aqueous two - phase systems. phase separation are promoted by increasing temperature and adding inorganic salts. different proteins are partitioned in different phases. the selectivity of protein partitioning is increased by adding ligands with affinity
報道了由正負離子表面活性劑與高聚物混合溶液形成的一種可用於蛋白質的分離及分析的新型雙水相萃取體系.研究了正負離子表面活性劑（溴化十二烷基三乙銨/十二烷基硫酸鈉）分別與葡聚糖和聚乙二醇混合雙水相體系的形成規律、相行為及牛血清蛋白和溶菌酶在雙水相體系中的分配.通過在高聚物分子中接上親和配基，研究蛋白質在雙水相體系中的親和分配.結果表明，在該體系中，表面活性劑與高聚物分別富集於不同相中.升高溫度及加入無機鹽均可促進雙水相體系的形成，不同蛋白質可分配于不同的相中.親和配基的引入極大地增強了蛋白質分配的選擇性Objective, clone tissue - type plasminogen activator ( t - pa ) gene and construct a new kind of recombinant vector containing human tissue - type plasminogon activator ( t - pa ) cnda neither cytotoxiaty nor actovating prot - oncogenes
目的：克隆組織纖溶酶原激活物（ t - pa ）基因並構建一種無細胞毒性、不激活原癌基因的真核表達的pcdna3 . 1 （ + ） / t - pa質粒載體。A single molecule of rta is able to depurinate 1500 - 2000 ribosomes per minute. ricin b chain ( rtb ) is a galactose - specific lectin which binds to the receptors on cell surfaces, and may enhance rta translocation by forming a pore in the membrane of intracellular organelles. ricin enters the cells by receptor - mediated endocytosis
內吞進入細胞的蓖麻毒素一部分返回到膜表面，一部分經早期內體至晚期內體，最後在溶酶體中降解，只有一小部分約5到達高爾基體，隨后逆轉運到粗面內質網，在那裡rta才和rtb解離，游離出有催化活性的rta 。Using pcr technology, a 2. 4kb dna fragment, part of tryptopanase operon, containing a promoter, a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e. coli k - 12, was cloned to pmd18 - t vector. the dna sequence is the same as which was published on science
為了證明質粒上的基因能表達出有活性的色氨酸酶，將這個dna片段克隆到pet28c質粒的bamhi和hind位點上，使該片段受t7rna聚合酶的啟動子控制，然後轉化噬菌體de3的溶源菌bl21 （ de3 ） 。分享友人