病原性基因 的英文怎麼說

中文拼音 [bìngyuánxìngjīyīn]
病原性基因 英文
virulence gene
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 原 : Ⅰ形容詞1 （最初的; 原來的） primary; original; former 2 （沒有加工的） unprocessed; raw Ⅱ動詞（原...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 病原性 : pathogenic microorganism
  • 病原 : （病因） etiology; aetiology aitiology; noxa （pl noxae）; cause of disease; pathogeny病原蟲 prot...
  1. Most of plants, when attacked by avirulent pathogens, usually produce a hypersensitive response ( hr ) at the locus attacked and develop a systemic acquired resistance ( sar ) throughout entire plant by expressing a set of defense genes including pathogenesis - related ( pr ) genes

    植物受到病原菌侵染后，感染部位產生過敏性壞死反應，在其它部位產生系統獲得性抗性並伴隨抗性基因包括病程相關蛋白（ pathogen - relatedprotein ）基因的表達。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Sedegah m, hedstrom rc, hobart p, et al. protection against malaria by immunization with plasmid dna encoding circumsporozoite protein [ j ]. proc natl acad sci usa, 1994, 91 ( 21 ) : 9866

    劉彥文，余新炳，徐勁等.惡性瘧原蟲海南株環子孢子蛋白基因的克隆與表達[ j ] .中國人獸共患病雜志， 2000 ， 16 （ 1 ） : 8
  4. This article reviews the clinical manifestations, mutation feature, gene location and phenotype of different ischemic cerebrovascular disease caused by monogenic disorders, including coagulation disorders, erythrocytic disorders, inherited small vessel disease, metabolic disorders, connective tissue diseases, vasculopathies and disorders of unknown etiology

    本文主要闡述了單基因遺傳障礙引起的缺血性腦血管病，包括凝血障礙、血細胞病、遺傳性小血管病、代謝障礙、結締組織病、大動脈病及不明原因引起缺血性腦血管病的臨床特徵、突變特點、基因定位及表型等遺傳學研究進展。
  5. Genomic analysis of parasitic human pathogens, particularly plasmodium falciparum, and leishmania major

    人類寄生性病原體的基因體分析，特別是瘧原蟲與利什曼原蟲。
  6. The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr, and express by protokaryotic and eukaryotic expressing system, then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine

    利用pcr方法擴增和克隆gpvh1分離株主要免疫原性蛋白基因vp3 ，並對其進行原核和真核表達，是建立小鵝瘟分子診斷方法、構建vp3基因重組禽痘病毒活載體疫苗的基礎，具有極為重要理論和實踐意義。
  7. To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7

    為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
  8. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  9. The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus

    本研究的目的在於以人的-干擾素基因（ ifn - ）作為目的基因，與鯉魚-肌動蛋白基因啟動子在體外重組，利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究，其結合了干擾素和基因工程育種抗草魚出血病病毒的優點，以期獲得對草魚出血病具有天然抗性的轉基因魚，並在此基礎上培育出草魚抗病新品系。
  10. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  11. The result shows that a vvibdv strain was obtained, the above work lay a important role for further studying on the molecular biological mechanism of antigenic drift and virulence variation of ibdv, molecular epedimiology, it also provided the basis for recombinant and gene deleted vaccine of ibdv

    本實驗可以幫助我們進一步探討ibdv抗原性漂移和毒力變化的分子生物學機制，追溯ibdv的起源，理解病毒的傳播方式。同時也為研製開發基因重組疫苗和缺失疫苗打下一定的基礎。
  12. The basic mechanism of viral attack is that the viruses replicate themselves using the host ' s in this case is " our " dna genetic replication system

    濾過性病毒能夠引起疾病的原因是，病毒能夠通過使用病人身體中的dna基因復制系統來復制自己。
  13. To elucidate the antigenic drift and evolution of h9n2 subtype avian influenza viruses ( aivs ), five isolates from the north of henan province during 1998 - 2002 were compared and analysed by cross - hemagglutinin inhibition test ( hi ), cross - virus neutralization test ( vn ) in the chicken embryo and chicken embryo primary kidney cell ( cek ) and cross protection against challenge infection test

    為了探討h _ 9n _ 2亞型禽流感病毒的抗原性有否發生漂移，本研究從生物學角度和ha基因分子水平上對1998 ? 2002年間在河南省豫北地區分離到的5株h _ 9n _ 2亞型禽流感病毒的抗原性變化進行了比較和分析。
  14. Recovery estimated from the safh4 plant line indicates that 9 ( jl g of pure active scfv can be obtained per gram of fresh leaf material, on a laboratory scale. the production of the scfv antibody proteins in plant root exudates was also addressed. the scfv antibody protein was continuously secreted from the transgenic tobacco roots into a simple hydroponic medium at 630 to 760 ng g - 1 dry weight of root day - 1

    在水培條件下，轉基因煙草根可連續分泌具有活性的重組抗乙肝病毒表面抗原pres1 （ 20 - 47 ）單鏈抗體進入到液體培養液中，不須破壞植物即可連續獲得重組單鏈抗體，為利用植物生物反應器連續生產單鏈抗體開辟了新途徑。
  15. Review on application of q - pcr on the detection of foodborne pathogenic microorganism

    基因晶元技術在病原性食品微生物檢測中的應用
  16. However, when they do infect chickens or turkeys, certain subtypes of lpai virus are capable of converting into hpai strains though genetic mutation

    然而，當他們感染雞或土雞時，部份的低病原（ lpai ）病毒可能會藉由基因變化，轉變成高病原性（ hpai ）株。
  17. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  18. For example, as a direct toxic effect, periodontal iruses and bacteria and their products ( endotoxins or enzymes, for example ) are toxic to surrounding cells and may directly induce mutations in tumor suppressor genes and proto - oncogenes or alter signaling pathways that affect cell proliferation or surial of epithelial cells

    例如，作為直接毒性作用，牙周病毒和細菌及在細胞周圍其產生的毒性物質（例如，類毒素或酶類）可直接誘導腫瘤抑制基因和原癌基因突變或者改變細胞信號傳導通路從而影響上皮細胞增殖或存活。
  19. For example, as a direct toxic effect, periodontal viruses and bacteria and their products ( endotoxins or enzymes, for example ) are toxic to surrounding cells and may directly induce mutations in tumor suppressor genes and proto - oncogenes or alter signaling pathways that affect cell proliferation or survival of epithelial cells

    例如，作為直接毒性作用，牙周病毒和細菌及在細胞周圍其產生的毒性物質（例如，類毒素或酶類）可直接誘導腫瘤抑制基因和原癌基因突變或者改變細胞信號傳導通路從而影響上皮細胞增殖或存活。
  20. The research at seoul national university in south korea demonstrates the principle of “ therapeutic cloning ” producing stem cells genetically identical to the patient, which could repair any damaged or diseased tissue in the body

    韓國漢城國立大學的這項研究論證了「治療性克隆」的原理，這種克隆能產生與病人的基因完全相同的幹細胞，可能修補人體任何受損或患病的組織。
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