磷酸化酶激酶 的英文怎麼說
中文拼音 [līnsuānhuàjī]
磷酸化酶激酶
英文
phosphorylase kinase-
Effect of filamin - c on extracellular signal - regulated kinase phosphorylation mediated by 1a - adrenergic receptor in hek293 cell
腎上腺素受體介導細胞外信號調節激酶磷酸化作用的影響Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways
我們發現，低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應，並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降， dna聚合酶譜發生改變，應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活，細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇，細胞信號轉導通路camp - pka - creb和jnk sapk被激活。According to the research of physiological and biochemical indicators or index, components of soluble proteins, substrate protein of phosphorylation and the activity of protein kinase in low - temperature stress in the leaves of brassica oleracea l., we tried to find the law of the physiological and biochemical response of brassica oleracea l. leaf to low temperature. at the same time, discussion on the signal transduction can also provide further evidences for revealing the mechanism of low - temperature stress. the results are showed as follows : malondialdehyde ( mda ), superoxide dismutase ( sod ), ascorbate peroxidase ( asp ) and peroxidase ( pod ) activities were changed greatly after 0 ~ 30min ' s treating with low temperature
本文以甘藍葉片為材料，通過對低溫5脅迫下甘藍生理生化指標、可溶性蛋白組分以及磷酸化底物蛋白、蛋白激酶活性的研究，以期找出甘藍葉片對低溫脅迫的生理生化響應規律，為甘藍露地越冬栽培防範寒害提供理論指導，同時對低溫脅迫下甘藍逆境信號傳導進行了探討，從而為徹底弄清低溫脅迫機理提供進一步的證據，研究的主要結果如下：丙二醛含量（ mda ） 、超氧化物歧化酶（ sod ） 、抗壞血酸過氧化物酶（ asp ）和過氧化物酶（ pod ）活性在低溫處理0 30min發生顯著變化，低溫處理3min后，甘藍葉片內mda含量基本沒有變化，處理5min時出現第一個峰值，達到對照的104 . 10 ， 10min出現低谷，僅為對照的86 . 27 ，隨后再次上升， 30min時超過第一峰值，為對照的113 . 93 。Protein kinase a is camp - dependent kinase, one of the most important signal transduction pathways, plays a pivotal role in growth, differentiation, tumor occur, cell cycle control, etc. pka activators such as camp, 8 - br - camp or phosphodiesterase ( pde ) inhibitor isomethyl butyl xanthine ( imbx ) or purified pka catalytic subunit all can inhibit germinal vesicle breakdown ( gvbd ) and meiotic maturation in mouse oocytes, also in xenopus oocytes
蛋白激酶a （ proteinkinasea ， pka ）是依賴于camp的絲蘇氨酸蛋白激酶，是重要的信號傳導途徑之一，廣泛參與許多生命過程，包括生長、分化、腫瘤發生、細胞凋亡、細胞周期調控等。以小鼠卵母細胞為實驗對象，給予pka激動劑camp 、 8 - br - camp或磷酸二酯酶抑制劑imbx （ isomethylbutylxanthine ）均可抑制小鼠卵母細胞的胚泡破裂及減數分裂成熟。Measured the protein content by lowery method and the activity of pkc by advanced takai method
蛋白激酶c是一種鈣活化的、磷脂依賴性的絲氨酸蘇氨酸激酶。Chloroplast phosphoprotein were first found in thylakoid membranes by bennett o the attachment or removal of a phosphate group from a protein may have profound effects on that protein ' s activities and properties, the reversible phospharylation of the membrane protein in thykaloid is a dymanic equilibrium process
而bennett （ 1977 ）發現的類囊體膜蛋白可逆磷酸化已成為近年研究的熱點之一，蛋白質連上或是移去磷酸基團都將強烈影響蛋白質的生理活性和性質，這需要類囊體膜蛋白激酶和磷酸酯酶的參與。The metabolism of these extreme microbes during the production of maotai liquor would further produce multiple enzymes of thermal stability such as amylase, protease, saccharifying enzyme, cellulose, glucase, xylanase, and each kind of dehydrase involved in redox reaction, and dna polyase etc
茅臺酒釀造過程中極端釀酒微生物代謝產生多種熱穩定性的酶，如澱粉酶、蛋白酶、糖化酶、纖維素酶、葡萄糖甘酶、木聚糖酶、參與氧化還原反應的各種脫氮酶、磷酸烯醇丙酮酸激酶及dna聚合酶等。In rat hippocampal slices, tbs induced phosphorylation of p21 - activated kinase ( pak ) and its downstream effecter cofilin, signaling proteins that enable actin filament growth
在鼠海馬切片中，短暫快速脈沖刺激導致蛋白激酶21的磷酸化，在它的下游區產生了cofilin ，一種能使肌動蛋白絲生長的信號蛋白。The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility
同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點，包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等，拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能，而磷酸化位點是arc1參與信號傳導過程所必需的。Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha
與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較，二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區，都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點，所不同的是： （ ）在結構功能域上， 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區； （ 2 ）在功能位點上， 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區，但沒有n糖基化位點； （ 3 ）擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性（ eElevation of intracellular calcium ions may be partly induced by increased influx through sarcolemma l type - calcium channels. intracellular calcium elevation, on one hand, would activate calpain, a calcium - dependent cysteine protease that degrade the myofibrillar proteins and cause muscle atrophy ; on the other hand, result in activation of calcineurin which enhance the activity of mhc i promoter and inhibit a shift of mhc isoforms from slow to fast in soleus
這樣，可能使得萎縮比目魚肌細胞內鈣離子水平升高，細胞內鈣離子靜息濃度的增加一方面激活calpain ，增加收縮蛋白的降解，使肌肉萎縮；草四軍醫大月卜祠成士學位論文另一方面通過激活鈣調神經磷酸酶，增加快型mhc基因的表達，使骨骼肌肌球蛋白重鏈（ mhc ）發生由慢型向快型的轉化。The combination of meja and fc did not show significant additive effect on enzyme activity. 3. phosphorylation and dephosphorlation of pm h ^ - atpase after treatments of meja and fc 1 ) in vitro, phosphatase inhibitors, okadaic acid and cantharidin, enhanced meja - induced increase of the enzyme activity to 60 % and 50 %, respectively
Tllrase過程中可能發生的磷酸化和去磷酸化作用1 ）磷酸酶抑制劑斑螫素、崗田酸可以增強meja對酶的刺激作用，崗田酸的效應較強，而斑鰲素的作用略弱，增幅分別為60和50左右。There was no difference in other biologic characteristic of mscs between the two separation method, such as cell anchorage ratio and clone formation ratio. ( 2 ) plga film presented uniformity frame with no protuberance and fissure under scanning electron microscopy ( sem ). big aperture with smooth wall and average 400 m i n size running - through each other was observed in porous plga substrate, around the big aperture there were many round micropores about 5 m size. all of the structure were equal and uniform, which satisfied the further research work. ( 3 ) mscs adhesion at earlier time was promoted by biotiegenrafter 3h, cell number was ( 1. 5 0. 18 ) 105 in the plga film coated with biotiegen group, which was significantly higher than that in plga film group ( p < 0. 01 ) and higher than that in coverslip group ( p < 0. 05 ), which cell number was ( 1. 04 0. 21 ) 105. after 6h and 12h biotiegen could not promote cell adhesion, and cell proliferation and alkaline phosphatase ( alp ) activity were not promoted dramatically during 9 days. ( 4 ) cell adhesion was promoted by fibronectin or collagen type i
G ） i型膠原、纖維粘連蛋白促進細胞增殖，細胞接種后3 、 6 、 gd三個檢測時間點，實驗組細胞均明顯高於對照組。與1型膠原相比，纖維粘連蛋白刺激作用更強。 ） i型膠原、纖維粘連蛋白尚能誘導mscs細胞向成骨細胞分化，不僅表達成骨細胞標志物ocn 、 alp 、 opnmrna ，而且堿性磷酸酶活性明顯增高，堿性磷酸酶及鈣結節7第四軍醫大學博士學位論文一染色均強陽性， i型膠原組mscs細胞堿性磷酸酶活性較fn組更高，有顯著性差異；同時，兔疫組化染色表明，經纖維粘連蛋白作用的mscs1型膠原表達陽性。The cyst cells enclosing spermatomeres maybe synthesize a kind of scf - like protein, which can recognize specially the c - kit receptor on the cellular membrane of spermatomeres. then c - kit is activated, dimerizing and autophosphorylating. at the same time, the tyrosine kinase domain of c - kit is activized, which phosphorylates the proteins that have sh2 domain
精母細胞周圍的囊細胞可能合成scf樣蛋白，特異地識別精母細胞膜上的c - kit受體，並刺激c - kit發生二聚化、自體磷酸化，激活胞內酪氨酸激酶活性，活化具有「 sh _ 2結構域」的靶蛋白，可能通過一系列信號級聯，最終激活與減數分裂的相關蛋白或基因。Oocyte maturation involves the activation of various signal trans - duction pathways that converge to activate maturation - promoting factor ( mpf ) ; this is a key activity that catalyses entry into m - phase of meiosis i and meiosis ii
在爪蟾卵中有大量未被激活的cdc2 - cyclinb復合物，稱為pre - mpf ， pre - mpf在tyr15位脫磷酸變成活性mpf ，起到這個作用的磷酸酶是cdc25 ， cdc25的活性通過磷酸化和細胞位置進行調控。Mpf is a complex that consists of the protein kinase p34cdc2 and the regulatory protein cycli b ; the protein kinase activity of p34cdc2 is strictly dependent on its association with cyclinb. in xenopus laevis eggs the absent of cyclin b, mpf will not be activited
Ca卜活化p34 」擬的機制第一步是結合cycinb ，並且處于磷酸化狀態，即thr14 、 tyrls和tlirl61都處于磷酸化狀態；第二步是cdc25磷酸酶通過是thr14 、 tyr15脫磷酸化而激活p34 」擬。( 4 ) phosphorylation modulation decreased activitis and temperature sensitivity of bkca channel reversibly, which suggested the participation of protein phosphorylation in the temperature regulation
通道在某一時間點的磷酸化狀態取決于蛋ei激酶的磷酸化、蛋白磷酯酶的之磷酸化之間的平衡。By combining the information from the cdna sequences with functions of their homologs, we suggest that two kinds of hormones may play important roles during flower regeneration in a few ways : ( i ) proteolytic system of ubiqutin / 26s proteosome complex, ( ii ) actin - dependent vesicular cycling of auxin efflux carrier, ( iii ) regulating transcription of genes, ( iv ) phosphorylation and dephosphorylation
經過進一步的篩選，結合序列特徵和同源基因的功能信息對這些基因功能進行了初步分析。認為兩種外源激素可能通過泛素26s蛋白酶體復合體對調節蛋白的降解機制，生長素運輸載體肌動蛋白依賴的小泡運輸，基因轉錄調節，磷酸化和去磷酸化等調節過程誘導風信子花分生組織的再生。Sh2 domain can specifically recognize phosphated tyrosine residue, and mediate receptor tyrosine protein kinase signal transduction
Shz結構域可以特異性識別磷酸化酪氨酸短序列，介導受體酪氨酸蛋白激酶信號轉? 2 ?導途徑。It has been demonstrated that kvl. 2, an isoform of delayed - rectifier potassium channel, is up - regulated in the ischemic neurons. besides, the activity of kvl. 2 is tyrosine phosphorylation dependent, phosphorylation of the tyrosine residue in the amino terminus of the channel by tyrosine kinases suppresses the channel current. therefore, we hypothesize that vegf could enhance the phosphorylation of kvl. 2, which results in an inhibition of / k
在介導i _ k的鉀通道家族中， kv1 . 2是其中的一個亞型，研究報道它在缺血神經元中的表達是增高的；此外，它的活性與其酪氨酸磷酸化水平相關，在其氨基末端的酪氨酸殘基經酪氨酸激酶磷酸化后可顯著降低其介導的電流的幅度。分享友人