篩序 的英文怎麼說

Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。

He had eleven children, all of whom were, almost from the moment they learned the alphabet, roped into the endless business of helping to sift through and alphabetize the several million slips of paper on which were recorded every twitch and burble of the language over seven centuries

他有11個孩子，所有的孩子差不多從學會字母那一刻起，就被這無休止的生意給纏住了- - -幫著篩選七百萬個紙片，並按字母表的順序進行分類，這些紙片記錄了七個多世紀所使用的所有字的來龍去脈

It is discovered that existence of small amount of n - butyl alcohol in mesoporous zeolite synthesis system can not only modify ordered degree of formation of mesoporous zeolite, which improves hydrothermal stability of it, but also help to attain much smaller, nearly nanometer, grain of mesoporous zeolite

研究發現，少量正丁醇的存在能改善介孔分子篩形成過程的有序度，並使其水熱穩定性得到提高，同時合成產物的粒度更小，趨近納米顆粒。

Calibration tests are standard procedures applied to sensors not eliminated by screening tests.

校準試驗是對篩選試驗中未被淘汰的傳感器進行試驗的標準測試程序。

The pollen can then be separated from the rest of the catkin by sieving through cheesecloth or fine screen.

以粗紗布或細篩過篩，便可將花粉同雄花序的其它部分分開。

In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。

Two spider silk protein genes ( avfl and avf2 ) screened from the library are characterized in a lot of repetitive motifs, a high guanosines or cytidines content, a strong preference for adenosine or thymidine in the third position of a codon and rich residues of glycines or alanines in the proteins translated

試驗結果，該文庫容量為4 . 9 10 ~ 6 。從文庫中篩選到avf1和avf2蛛絲蛋白新基因，具有典型重復序列多， g c含量高，密碼子第三個堿基偏愛使用a t及編碼蛋白中含有大量gly和ala殘基等特點。

The following diagram illustrates the filtering common to ffa applications

下列關系圖說明了常用於ffa應用程序的篩選：

In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。

Then the pcr product was purified, ligated into pgem - t vector by ta cloning

篩選陽性克隆，測序，大量制備序列完全正確的質粒。

The rasterizer uses the magnification filter instead

光柵化程序改用放大篩選器。

This machine adopts one pair of rotating grinding blade to grind form damp material into column - shape granule by stainless steel sieve cylinder which is provided for pelletize in next process

本機是通過一組旋轉碾刀，將前道工序製成的濕材注入環碾刀的不銹鋼篩筒篩孔中，從而製成圓柱狀的濕顆粒，供下道工序拋丸使用。本設備可通過更換不銹鋼篩筒來取得不同大小的顆粒。

Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis

二、研究論文1 、參照人sry基因hmg - box保守區的序列，設計一對兼并引物， pcr擴增了中華絨螯蟹的sox基因，並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ，其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ，顯示該基因在進化上具高度的保守性。

In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。

Can be resumable, which means a filtered exception handler can correct the problem that caused the exception, and the code will continue from the point that threw the exception

可以恢復，這意味著篩選異常處理程序可以更正導致異常的問題，並且代碼將從引發異常的地方繼續。

This paper analysis the data mining of the single nd multiple streams time series, and draw a conclusion that the relationship between the events of the multiple streams time series are the association patterns dependency patterns, sudden patterns, this paper call them are structure patterns, the existing algorithm have n ' t discuss these patterns, although msdd discussed the dependency patterns, however, it ignored the association patterns, sudden patterns, this paper have a definition of the association patterns, sudden patterns and dependency patterns, and have a complete, frank algorithm called twma ( time window moving and filtering algorithm ), the peculiarity of this algorithm is that events is listed by the time window, by this way, the relationship of the events is clear

本文將它們統稱為結構模式，而這正是目前其它演算法、沒有考慮到的，雖然msdd考慮了事件之間的依賴關系，但它忽略了突變模式，關聯模式等重要的知識表示。本文給出了關聯模式、依賴模式、突變模式的定義，提出了一個比較靈活全面、直觀的挖掘它們的演算法：時間窗口移動篩選演算法twma （ timewindowmovingandfilteringalgorithm ） 。該演算法的一個突出特點是將時間序列事件按時間窗口序列化，使得事件之間的時間關系表示很直觀，該演算法能成功地從多流時間序列中發現了事件之間的關系。

You can control the case sensitivity of filtering, searching, and sorting by setting the dataset s

屬性，可以控制篩選、搜索和排序是否區分大小寫。

Which shared 10 % ~ 73 % identities to other rips from plants and 37 % ~ 73 % to other rjps from cucurbitaceae. six novel rip gene fragments ( 408 ' bp ), 1 from benincasa hispida and 5 from cucurbit a moschaia

根據葫蘆科rip上、下游兩段高度保守的氨基酸序列設計簡並引物對ly1 ly2 ，對基因組dna進行pcr擴增，首次建立了rip新基因快速篩選體系。

Adding message filters to the message pump for an application can degrade performance

向應用程序的消息泵添加消息篩選器會降低性能。

At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。