結合酶 的英文怎麼說
中文拼音 [jiēgě]
結合酶
英文
conjugase-
The different isozymes bind calcium ions with different affinities.
不同的同功酶以不同的親和力與鈣離子相結合。Purer acetylcholinesterase was obtained through salting out by ammonium sulphate and ion exchanging
分別採用雞蛋膜固定化法,活化載體共價結合法和戊二醛交聯法進行酶的固定。In these chloroplasts carbon dioxide combines with phosphoenolpyruvate to form oxaloacetic acid, which is transported to the bundle sheath cells, where the carbon dioxide is released, then fixed by the enzyme ribulose bisphosphate carboxylase to form glycerate 3 - phosphate, the first step in the calvin cycle
在葉肉細胞的葉綠體中二氧化碳與磷酸烯醇丙酮酸結合形成草酰乙酸,后被運到鄰近的維管束鞘細胞,在那裡二氧化碳被釋放,后被核酮糖二磷酸羧化酶固定形成3磷酸甘油酸,這是卡爾文的循環第一步。Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants
血吸蟲病疫苗研究已有60多年的歷史,大致經歷了死疫苗、活疫苗(照射致弱尾蚴疫苗)和基因工程疫苗等研究階段,產生了一些who / tdr推薦認為很有希望的疫苗候選分子,如谷胱甘肽- s -轉移酶( gst ) 、副肌球蛋白( sm97 ) 、照射致弱疫苗抗原5 ( irv - 5 ) 、磷酸丙糖異構酶( tpi ) 、曼氏血吸蟲膜內在蛋白( sm23 )和脂肪酸結合蛋白( fabp , sm14 )等,但其對宿主的保護作用均不甚理想,未能穩定地達到40或以上的保護力水平,因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑,進一步提高其保護力。When a catalytically active enzyme forms a complex with a cofactor a holoenzyme is produced
酶蛋白和輔助因子兩者結合成完整分子時,形成具有活力的全酶。6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析, blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟,得到聚丙烯酰胺凝膠電泳為單一蛋白區帶,比活為1 . 46u mg的酶制劑。Notch interaction with its ligands induces the cleavage of its intracellular domain ( ic ), and the notch ic translocates to the nucleus and binds to rbp - j, the mammalian homolog of su ( h ), to transactivate transcription of target genes such as e ( spl ) ( enhancer of split ), hesl ( hairy and enhancer of split ) and hes5 four notch receptors and their ligands are differentially and redundantly expressed in a variety of vertebrate tissues
它通過其識別序列( cogtgggaa )結合於受調控基因的啟動子區,在轉錄激活因子的驅動下調節細胞分化和個體發育相關基因的表達。在沒有n 。 tch胞內段的情況下, rbpj可與包含sm盯( silencingmediatorforretlnoldandthyroidhormonereceptor )和組蛋白去乙酞化酶的轉錄輔助抑制復合物結合,當notch信號被激活時; rbpj可與n 。For uncovering the effects of reversible phospharylation on the structure and function of psii reaction centre, we purified a protein phosphatase associated membrane from the thykaloid membrane of ipomoea aquatica chloroplasts. in our experiments we studied the enzymology and spect rum characters of the purified phosphatase. in our lab, one kind of protein phosphatase associated thylakoid membrane of pomoea aquatica has been isolated
迄今人們對類囊體蛋白磷酸酯酶的研究較少,為了研究可逆磷酸化對psii反應中心結構與功能的影響,本文以蕹菜為材料,從葉綠體類囊體膜中分離純化到一種膜結合蛋白磷酸酯酶,進行了酶學性質和光譜性質的研究。Dr kornberg worked out the details by crystallising the complex of dna, rna and polymerase at various stages of the process
考恩伯格博士通過對此過程中各個階段的dna 、 rna和聚合酶的復合體進行結晶,研究出這一過程的詳細情況。Ligase an enzyme that catalyzes the bond formation between two substrates at the expense of the breakdown of atp or some other nucleotide triphosphate
連接酶:可將兩個底物結合在一起的酶,此過程需要atp或其他核苷三磷酸供能。Active lipase is associated with the membrane of the spherosomes.
活躍的脂肪酶與圓球體的膜相結合。The masp ( mannose - binding lectin - associated serine protease ) gene has been cloned by the method of degenerative pcr and the fragment of the pcr product is 630 bps in length
本文還利用pcr方法從青島文昌魚基因組dna中克隆masp (甘露聚糖結合凝集素相關絲氨酸蛋白酶)基因片段。The results of these early research work showed that rna polymerase transcription was localized in the nucleoli and rna polymerase and in the nucleoplasm
當時的研究結果顯示: rna聚合酶的轉錄發生在核仁內, rna聚合酶和rna聚合酶的位於核質中。It was showed under the laser scanning confocal microscopy that : for dna level fish, 81 % of the dnas were in the nucleoli and at the periphery of the nucleoli and 19 % in the nucleoplasm ; for rna level fish, 22 % of the rnas were in the nucleoli, 78 % in the nucleoplasm and at the boundary between it and the nucleoli ; for dna - rna level fish, 25 %, 46 % and 29 % of the dnas or rnas were in the nucleoli, at the periphery of the nucleoli and in the nucleoplasm, respectively
結果如下: dna水平熒光原位雜交結果顯示, 81的dna位於核仁內部及其核仁周邊區域, 19的位於核質中; rna水平熒光原位雜交結果表明, 22的rna位於核仁內, 78的位於核質及與核仁交界處; dna - rna水平熒光原位雜交結果是, 25的dna或rna位於核仁內, 46處于核仁周邊, 29位於核質中。由此推測出, rna聚合酶的轉錄主要發生在核仁及其周邊區域。It is inferred that its active transcription occurs in the same region, not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rna polymeraes
這一結果不僅直觀地向人們表明了rna聚合酶在真核細胞核中的轉錄位點,而且對於人們進一步認識和理解rna聚合酶的轉錄機制、其轉錄產物的加工運輸途徑、以及真核細胞當中不同的rna聚合酶間的組織和調控關系都將有著重要的理論意義。From these results, it is inferred that the active transcription of pol iii occurs in the nucleoli and its periphery region, but not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase iii transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rnapolymeraes
本實驗為rna聚合酶在真核生物細胞核中的轉錄位點提供了較為直接的證據,這對人們進一步了解rna聚合酶的轉錄機制、加工和運輸過程及三種rna聚合酶之間的結構與功能關系具有重要的意義。The results of these early research work showed that rna polymerase iii transcription was localized in the nucleoplasm. however, with the development and the application of new technologies since 1990s, the controversy arose on the transcription sites of rna polymerase iii. in recent years, more and more scientists presumed that rna polymerase iii transcription might not occur in the nucleoplasm but in the nucleoli
自上個世紀八十年代初期,人們相繼運用細胞化學染色、電鏡放射自顯影等進行研究的結果表明: rna聚合酶的轉錄發生在核質中,但隨著新的研究技術的發展和應用,人們卻發現rna聚合酶的轉錄可能發生在核仁中,從而對早期的研究結果提出了質疑。Almost one - third of all proteases can be classified as serine proteases, including complement subcomponent clr / cls, mannose - associated serine proteases ( masps ), ovochymase, spermadhesin, type ii transmembrane serine proteases ( ttsps ) etc. these proteins are involved in diverse biological processes, including developmental processes such as complement activation, ovulation, fertilization, tissue remodeling, cellular migration, cancer invasion and metastasis, intestinal digestion, embryogenesis, or organogenesis
絲氨酸蛋白酶( serineprotease )是機體最重要的酶分子之一,約占機體蛋白酶的三分之一,我們較熟知的絲氨酸蛋白酶就包括補體組分c1r c1s 、甘露糖結合絲氨酸蛋白酶、 ovochymase 、 spermadhesin和型跨膜絲氨酸蛋白酶等,它們參與了補體活化、排卵、授精、組織重建、細胞遷移、腫瘤浸潤和轉移、消化、胚胎發育、器官形成等多項生理功能。The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )
以抗h3n2流感病毒的多克隆抗體( 100ng l )包被酶標板,加入制備好的肽庫,用tbst洗去非特異結合的噬菌體,加0 . 2mol l甘氨酸-鹽酸( ph2 . 2 ) ,室溫放置10min以洗脫特異結合的噬菌體, 2mol ltris - hcl ( ph9 . 1 )中和后,取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定,其餘噬菌體擴增後用于下一輪篩選,共重復3輪淘洗。To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長,共有10799個堿基,故此我們將mint分為六段,分別命名為fi一f6 ,本研究以其中的f5 ( 222e959 )和f6 ( 296s576 )片段為研究對象,將h者分別插入pgb盯7載體中,結果顯示: mintfs可與核受體輔助抑制因子1階cori ) , o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用,而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。分享友人