缺失相 的英文怎麼說
中文拼音 [quēshīxiāng]
缺失相
英文
a ent phase-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。If one is prone to losing notes in their language of light scale due to karmic encounters, and detects so through muscle testing or use of the pendulum, then one may call upon the associated mineral kingdom for assistance in replacing the missing note along with learning to master each note more fully
如果你由於業力遭遇而容易丟失光之語音階中的音調，通過肌肉測試或用擺錘來檢定，隨后你能呼喚相關的礦物王國，來幫助你重置入缺失的音調，及學會充分掌握每一個音調。Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank
以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。After analyzing the reasons of credibility gap, the article proposes that the government should : ( 1 ) enact and perfect the laws and rules relevant to credit management, strengthen the management and execution, and provide good circumstances for the enterprise credit management through perfecting the macro - control system ; and ( 2 ) on this basis, combine china " s enterprise credit management situation with occidental advanced enterprise credit management theory, and carry out comprehensive and systematic credit management
文章通過剖析企業信用缺失的原因，然後提出：一方面政府要建立、健全信用管理相關的法律和制度，加強管理和執法力度，通過國家宏觀制度的完善為企業信用管理創造良好的環境。在此基礎上，另一方面要將西方先進的企業信用管理理論和我國企業信用管理實際狀況相結合，對企業實施全面系統的信用管理。Institutional disappearance and practice disorganization is the impetus to study relevant legal problems of enterprise trusteeship
制度性缺失導致的實踐失范是研究企業託管相關法律問題的動力所在。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins
將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測，發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分，阿維菌素的總產量和b1的產量與出發菌株相當，說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。There exist successive deletions in the sequence of the genomic dna of kale and kohlrabi, compared with that of brussel sprouts
相對抱子甘藍基因組dna而言，羽衣甘藍和球莖甘藍在序列中發生了多處大量堿基的缺失。Meanwhile, the results show that sp - d, sp - j, sp - z and sp - c are relatively tolerant to lincomycin, but the other 6 strains of meterials are not
7kd的一條蛋白帶，而直線形藻絲體即mo則缺失此帶，表明此53 7l蛋白與螺旋藻的形態變異相關。Part iiilegal issues upon the reform of toll and tax in countryside this part is concentrated to show six problems that the reform of toll and tax in countryside is to be up against and is to be peremptorily settled as following : the first one, in the confirming of agricultural tax, it is very difficult to protect the farmer ' s rights fairly because of unscientific of the evidence at assessment, highly of tax rate, and the absence of law and statute correlatively ; the second one, on the tax levying, there lack of legal systems that is to supervise and run, especially lack of law and statute which lead to collect agricultural special tax canonically ; the third one, the reform of toll and tax in countryside extrudes unbalance burden among farmers and countries, and lead to unevenly between subject of tax payment, which breaches the legal rule of balanceable tax ; the fourth one, the reform of toll and tax in countryside faces the danger that farmers " burden tends to rebound because of weakly carry through legal rule on tax statutory and absent of law and statute correlatively ; the fifth one, the reform of toll and tax in countryside affects the finance and revenue - expenditure auditing of the grass roots and father affects the grass roots to raise fee upon public construction and education, as a result, there ' s more charge that is out of law, the monocracy of grass roots " finance ought to be carry out ; the sixth one, legal measures connect with the reform of toll and tax in countryside in dire need of to be built, which mainly conclude legal system that adjust country social security, country compulsory education and country debt
第三部分? ?農村稅費改革的法律課題集中展示了農村稅費改革所面臨且亟待解決的六大法律問題。即：其一，在確定農業稅上，計稅依據不科學、稅率偏高、相關法律規范缺失，無法平等保護農民的合法權益；其二，在農村稅收的徵收上，缺乏行之有效的法律監督和運行機制，尤其是農業特產稅的徵收缺乏保障據實徵收的法律規范;其三，稅費改革凸顯農民之間、農村之間的負擔不均，使納稅主體的權利義務失衡，有悖稅負均衡的法律原則;其四，由於稅收法定主義沒有得到有效貫徹，規制農民負擔反彈方面的法律法規缺位，致使農村稅費改革潛伏著農民負擔反彈的風險;其五，稅費改革影響基層組織的財政收支，進而影響鄉村公益事業建設及教育經費的籌措，引發稅外亂收費沉渣泛起，基層財政體製法治化勢在必行;其六，與稅費改革相關的配套法律措施巫待構建，主要是農村社會保障法律體系、鄉村義務教育和鄉村債務疏導的法律支撐魚待構建與完善。第四部分一一農村稅費改革與相關法律制度的構建基於上一部分提出的法律問題，力圖構築農村稅費改革的法律框架並提出相應對策。Here we found g proteins also function in leaf, silique development and the yield of pollen microspore. we observed several traits or characters in the offsprings of gpal, agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type, whereas the lamina length, petiole length and rosette diameter is smaller than the wild type, the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type, and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines
實驗中我們跟蹤觀察了多代異三聚體g蛋白a亞基超表達轉基因植株及a ， p亞基缺失突變體的表型特徵，發現突變體的葉片寬度大於對應的野生型，葉片長度，葉柄長度及蓮座直徑小於野生型，而超表達植株的上述某些特徵與突變體相反; gp時突變體的長角果長度，花梗柄部長度大於野生型，而超表達ga植株種英則略小於對照; gpal突變體長角果尖端未出現咭乙i突變體的特徵: gpal ，口gbl突變體花粉生成量大於野生型，而超表達ga植株的花粉生成量則略小於對照。During the process of the rapid economy transformation in china, due to the lagging of the system arrangement, systematic credibility shortage comes forth, especially in the field of the real estate appraisal. " unfair appraisal, skullduggery together " abounds, which has become the hidden trouble of the economy running, and endangered the security of the national finance system
摘要中國在經濟快速轉型的過程中，由於制度安排相對滯后，出現了系統性的信用缺失，在房地產估價業尤顯突出，大量存在的「高估低評、合謀作假」 ，成為宏觀經濟運行的隱患，並將危及國家金融安全。Do you believe that you can make the stegosaurus come back to life if you find all the missing bones and put them in the right place of the stegosaurus body
只要找到全部缺失的骨頭，並把它們插到劍龍身上相應的位置，信不信，你就能使它復活？The description of performance and basic environment of foreign vc in china indicates though china has stable economic development basis and certain conditions for foreign vc, the overall investment environment is not adequate. such as conflict of laws, imperfect business circumstance, ignorance of foreign vc practice for local target companies and lack of vc exit mechanism
從對我國吸引外國風險投資的狀況和基本環境介紹可以看出，我國雖擁有穩固的經濟發展基礎和一定的吸引外國風險投資條件，但仍存在許多不足之處，包括涉及風險投資的相關法規存在不適應方面、風險投資商業環境不夠完善、風險企業對外國風險投資運作方式認識不足、外國風險投資在我國缺失靈活退出通道等。Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions
細胞色素b基因序列變異分析表明： 1 ）雁形目鳥類細胞色素b基因全序列長度一致，無插入和缺失：對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列，無無義突變，全序列內部無終止密碼子； 2 ）序列比對后1143加，含416個核著酸變異位點， 306個簡約信息位點，其中處於密碼子第三位的變異最大，第一位和第二位堿基的變異相對較小。Based on the deletion region, the possible candidate genes of 20 stocks were searched in flybase. they mainly function as transcription factors, tran slation factor, kinase, signal molecules, and so on
根據缺失區段，在果蠅數據庫中檢索了7個品系相關的可能候選基因，它們的功能主要有轉錄因子、翻譯因子、激酶、和信號分子等。It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain
通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段，將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較，結果在參比的16個ndv毒株中在該區段中除了有多個點突變外，個別毒株有堿基插入和缺失，所有以lasota株為代表的弱毒株均無6堿基的插入，而以f48e9株為首的強毒株均有此6堿基的插入，但有一個中等毒力的毒株dp沒有6堿基的插入，不過它的基因序列和lasota的幾乎相同，對于所克隆到的基因的代表性還有待確定。This article started with the background of " 3 + x " model, aimed to explore the conception and the essence of " 3 + x ", seeked for the gap between this model and actual biology teaching, disclosed the drawback of " education for tests " that existed in both idea and practice of teaching as well as the lack of role consciousness, efficiency and innovation, and consequently presented relevant teaching strategy and measures
本課題從「 3 + x 」高考模式產生的背景中追根搠源，探討「 3 + x 」的命題立意及實質，從實際的中學生物學教學現狀的調查研究中找尋與之不相適應的差距，發現了教育教學中從觀念到實施所存在的「應試教育」的遺患，以及課堂教學中存在的主體性、有效性和創新性缺失等問題，進而提出了相應的教學策略和措施。The development of society and economy of shanxi province falls behind the advanced regions of china because of the factors of history and natural situation. so the most of the cities of shanxi province are small, and not integrated, and there are few big cities in shanxi province, which makes the medium - sized cities act a very important role in the social economic development of shanxi province
另一方面，由於受歷史及自然條件的影響，陜西社會經濟發展與東部發達地區相比，仍顯滯后，陜西城市的規模大多較小，城市規模體系不完整，大城市缺失，陜西中等城市在陜西社會經濟發展中的地位益加突出。Compared with bombyx. mori, the exon regions of alpha - amylase gene of bombyx mandarina have 13 transitions, 4 transversions and a 12bp insertions, while the intron regions have 362 transitions, 343 transversions, a 94bp insertions and a 56bp deletions. the whole alpha - amylase gene ( including the 5 ' - and 3 ' - utrs ) of bombyx mandarina has 380 transitions, 350 transversions, 113bp insertions and 57bp deletions
野桑蠶相對於家蠶來說，在-澱粉酶基因外顯子區域發生了13次轉換、 4次顛換、 12bp插入，在內含子區域發生了362次轉換、 343次顛換、 94bp插入和56bp缺失，全基因（包括utr ）共發生mmrttoopmi mgzx ffatt了380次轉換、 350顛換、 113hp插人和57hp缺失。分享友人