胚胎原基 的英文怎麼說
中文拼音 [pēitāiyuánjī]
胚胎原基
英文
embryonic primodia-
Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %
採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵，實驗結果如下： （ 1 ）顯微注射后，根據胚胎發育分期，胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光，原腸期發熒光的胚胎比例為25 ，後期發育熒光率逐漸下降，肌肉效應期后又相對穩定。For the cryogenic preservation of fish, in this paper we made the primary culture of the kidney of allotetroploid crucian carp and primary studies were carried out on cryopreservation culture offish embryo cells derived from misgurnus auguillicaudatus or grass carp, the results of the experiments are as follows : the primary cell culture of the kidney tissue derived from allotetroploid crucian carp was carried out using tissue adherent culture, the primary observations of the growth conditions and morphology of the primary culture and subculture cells which originally come from the kidney tissue were also made
本文主要從魚類種質保存的目的出發，一方面以四倍體鯽鯉魚為材料，對四倍體鯽鯉魚腎臟組織進行初步培養，為建立相應細胞庫及下一步培養凍存的魚類胚胎細胞奠定基礎；另一方面，以魚類組織細胞培養技術為基礎，泥鰍胚胎細胞為材料，對魚類胚胎細胞凍存培養方法進行初步研究，並應用該技術方法對草魚早期胚胎細胞進行凍存培養實驗。報告如下：本文用組織貼壁法對四倍體鯽鯉魚腎組織進行原代、傳代培養。Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件，以hprt為陽性對照，用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析，結果發現，轉錄sry基因的睪丸組織以及雄性胚胎，從受精卵發育到囊胚的過程中，基本上不轉錄xist基因；不轉錄sry基因的雌性卵母細胞和雌性胚胎，從出現原核開始，到發育至2 -細胞期的過程中， xist基因一直不轉錄，但是，從4 -細胞期開始，一直到孵化前囊胚階段，雌性胚胎都轉錄xist基因。The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時，還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試，並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學，細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞，但其遺傳學和細胞生物學背景知之甚少。The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy. after mechanical desection and enzymic digestion, the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin. the medium contains several cytokines : lif ( leukemia inhibitory factor ), bfgf and foskolin
從4 ? 8周的流產胎兒的原始生殖嵴部位分離到原始生殖細胞，經過機械和化學方法的解離后培養於事先用絲裂黴素處理過的小鼠胚胎成纖維細胞或人胚胎成纖維細胞飼養層上，培養基中添加了lif ， bfgef ， foskolin等細胞因子，此後大約每7天左右傳代一次。By this method of primary culture, cells and tissues could not only subsist for 11 months and more in vitro, but also be subcultured. these works lay a foundation of the establishment of silkworm embryonic cell line
為建立新的家蠶胚胎細胞系奠定了一定的基礎。原代培養的細胞和組織塊在體外有一定的生長現象，細胞體外最長存活達門個月以上。The objective of this study was whether the reconstituted embryo with the second polar body ( pb2 ) can develop. firstly, female mouse was superovulated with pmsg + hcg and keep in the same cage with male mouse, the pronuclear embryo was obtained at the different time after hcg injection with different motheds to investigate the existing time of pronuclear embryo. then pronuclear embryo was cultured with three different mediums to select the best to culture in vitro
母鼠在注射hcg后與公鼠同籠交配，並在注射hcg后不同時間（從18h到26h ） 、不同方法（體內法和體外法）獲取胚胎，研究獲取原核胚的最佳時間；在此基礎上獲得具有第二極體原核胚，然後對原核胚進行顯微操作，去除雌原核並注入第二極體，探討第二極體內遺傳物質支持胚胎發育的潛力；同時利用dna特異性染料h33342對原核胚進行染色，顯示並比較原核和第二極體內的dna遺傳物質。The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates
加入新鮮的eg細胞培養液（含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分）培養24小時以後， pgcs開始部分貼附於共培養的生殖原基細胞上。In order to clone new genes expressed during early embryonic development of trionyx sinensis, we constructed and characterized a cdna expression library from poly ( a + ) mrna isolated from 250 mg of cranial / kidney / gonad complex tissues of one - week - old embryos of trionyx sinesis using the smart ( switching mechanism at 5 " end of rna transcript ) cdna synthesis and ld - pcr amplification strategy
為了克隆到與胚胎發育有關的新基因，以孵化一星期的中華鱉（ trto屍砂優sinensis ）胚胎的頭部、生殖晴混合組織為原始材料，採用smart但witching叢eehanism叢5 』 endof旦nairanseript ）和長距離pcr伍d一pcr ）技術，構建t一個中華鱉cdna表達文庫。Embryonic stem ( es ) cells were derived from the inner cell mass ( icm ) of preimplantation embryos, and embryonic germ ( eg ) cells from primordial germ cells ( pgc ). both es and eg cells were cultured to determine the factors affecting on isolation, cloning and passage of pluripotent stem cells from icr mouse embryos
試驗以icr小鼠胚胎內細胞團（ icm ）和原始生殖細胞（ pgc ）為材料，採用不同培養體系分離克隆胚胎多能幹細胞，探討icr品系小鼠胚胎多能幹細胞分離克隆的基本方法，以及一些因素對其分離、培養、克隆、傳代等效果的影響。Howeer, uniparental es cell research faces the biological hurdle of genomic imprinting, in which specific gene expression patterns are dictated by the parental origin
然而，單系胚胎幹細胞研究面臨著基因組印跡的生物學障礙，即特殊的基因表達模式受原始親代的控制。At present, specialized courses offered in secondary health vocational schools have been tending towards comprehensiveness and appear to be more wide spread and more practical. that is more useful to the medical students facing basic levels. for example : anatomy, physiology, histological and embryology has been merged into normal humanbody
目前中等衛生學校專業課程設置已越來越趨于綜合性，出現「寬」的特徵，體現出對于面向基層第一線工作的醫學生更具有實用性，如原有的《解剖學》 、 《生理學》 、 《組織胚胎學》等學科合併為《正常人體學》 。Firstly in mammary epithelial stem cells, we investigated that there were some cells to express the specific genes, defined in embryonic stem cells or other known stem cell lineages, such as oct - 4, rex - 1, integrinb1, bcrp1 / acbg 2 and cd 34. 6. estabished an optimal cultural system for enriched mammary epithelial stem cells, firstly
首次證明體外短期培養的乳腺類腺體體系中，有一些細胞可以表達一定量的胚胎幹細胞的特異性表達抗原基因oct - 4 、 rex - 1 ；上皮幹細胞的特異性表達抗原基因integrin - 1 ；大部分已知幹細胞共表達的抗原基因bcrpl acbg21 ； 5分享友人