菌體抗原 的英文怎麼說

中文拼音 [jūntǐkàngyuán]
菌體抗原 英文
o antigen
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 體 : 體構詞成分。
  • 抗 : Ⅰ動詞1 （抵抗; 抵擋） resist; combat; fight 2 （拒絕; 抗拒） refuse; defy 3 （對等） contend with...
  • 原 : Ⅰ形容詞1 （最初的; 原來的） primary; original; former 2 （沒有加工的） unprocessed; raw Ⅱ動詞（原...
  1. Results indicate that lactobacillus casei possesses probiotic properties and can be used as oral vaccine carrier to deliver heterologous antigen

    結果表明：乾酪乳桿菌符合益生菌的標準，可作為遞呈疫苗抗原活菌載體。
  2. The monoclonal antibodies ( mab ) specific for the nucleoprotein of prrsv were used to select a phage random heptapeptide library

    採用噬菌體隨機7肽庫對單克隆抗體ge3識別的抗原表位進行了鑒定。
  3. In order to produce monoclonal antibodies, first, several v. dahliae isolates were grown in liquid czapeak medium, after rinsing mycelia and eliminating zoospores, the fungal tissue was homogenized with the pestle in liquid nitrogen and then transferred to test tubes and was centrifuged in tris - hcl buffer

    在制備抗原的過程中，首先液體振蕩培養了若干株棉花黃萎病菌，經過沖洗除孢子、液氮研磨，用tris - hcl抽提，再離心制得菌絲蛋白提取液，可作為電泳樣品。
  4. Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use

    X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。
  5. Phage display describes a selection technique in which a peptide or protein is expressed as a fussion with a coat protein of bacteriophage, resulting in display of the fused protein on the surface of the virion, while the dna encoding the fussion resides within the virion

    自1985年gpsmith首次提出噬菌體展示技術以來，隨著生物技術的發展，噬菌體隨機肽庫已成為研究分子間相互作用的有力工具，特別是在抗原表位研究方面。
  6. The diaphragm had the ability to detect the positive serum when it was diluted to 2 ' 11 and so it has good sensitivity ; stored at 4 for at least 7 months, the sensitivity and specificity of the diaphragm did not change, so it has good stability ; when 10 positive serum was detected 3 times, the result is reproducible, so the diaphragm has good reproducible. serums from experimental inoculated piglets was detected. the results showed that when the titer is l : 16, the pigs were infected with streptococcus suis ; and when 1 : 64, the pigs could survive after challege with streptococcus suis. all the results have shown that dot - ppa - elisa was a convenient, rapid, sensitive specific useful method for the detection of antibody

    該法以硝酸纖維素膜為固相載體，包被膜載抗原製成的診斷膜片具有良好的特異性：不與仔豬副傷寒、豬巴氏桿菌病、豬大腸桿菌病、豬衣原體病、豬瘟、豬細小病毒病、豬偽狂犬病、豬布氏桿菌、豬丹毒陽性血清反應；膜片具有良好的靈敏性，陽性血清作2 ~ （ - 11 ）稀釋亦能檢出；膜片具有良好的穩定性，在4至少能保存7個月，其靈敏性不變。
  7. In addition, no back mutation to obtain a maximal complexity ( i. e. - resistant and sensitive species coexistence ) or original ecology ( i. e. original - lysogen ) for the evolution occurs

    而且，逆反應變異回原有菌相而成對噬菌體敏感及阻抗菌相共存之最大分歧度或回歸至起始單一對噬茵體敏感菌相併未曾演化發生。
  8. We are now working on the expression and oral vaccination of the hepatitis b virus surface antigen and nucleic antigen by using the expression system in lab

    正在利用這一表達體系研究乙肝表面抗原和核心抗原的在乳酸菌中表達及口服免疫。
  9. In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit

    本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。
  10. A protein substance produced in the blood or tissues in response to a specific antigen, such as a bacterium or a toxin. antibodies destroy or weaken bacteria and neutralize organic poisons, thus forming the basis of immunity

    抗體，免疫體血液或組織針對某種抗原如細菌或毒素而產生的蛋白質物質。抗體消滅細菌或削弱其作用，中和有機毒素，因而形成免疫力
  11. Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag

    目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。
  12. Ptxb1 - hng and ptxb1 - m - insulin are expressed in e. coli successfully. after sds - page and densitometric scan analysis, the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins. western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )

    Pbv220 ? hng在大腸桿菌中未檢測到表達，后兩個克隆在大腸桿菌bl21 （ de3 ）中獲得高效表達， hng及m - insulin融合蛋白表達量分別佔全菌蛋白的40及50左右；經western - blot鑒定m - insulin融合蛋白可以與小鼠抗人胰島素單克隆抗體（ igg ）發生抗原抗體結合反應。
  13. And the positive clones were used as the immunogens to immunize mice. both of the serum and phage peptides were add to measure their effects on the growth of huvec ( human umbilical vascular endothelial cell )

    檢驗這種短肽可否模擬vegf的抗原決定簇誘發機體產生能與vegf結合的抗體，研究小鼠抗血清對huvec （人臍靜脈血管內皮細胞）生長的抑制作用以及噬菌體表達的7肽和12肽對huvec生長的抑制作用。
  14. Different kinds of salmonella antigens have been tried and an improved heat - extracted antigen was used as the diaphragm antigen. the optimum condition of dot - ppa - elisa was determined. the antibody positive standard of salmonella infection was established as 1 : 32, and the positive standard of protecting pigs from salmonella infection as 1 : 512

    研究中對多種方法提取的沙門氏菌抗原進行了篩選，選取了特異性良好的抗原；確定了dot - ppa - elisa的最佳工作條件；初步確定了感染仔豬副傷寒的dot - elisa抗體陽性標準為1 ： 32 ，豬只抵禦副傷寒感染的dot - elisa抗體保護標準為1 ： 512 。
  15. A dot - ppa - elisa has been developed to detect the specific antibody against streptococcus suis. the antigen was isolated from group c d e r and type 2 streptococcus suis by three different method : ( a ) autoclave extraction method, ( b ) hcl - extraction method, and ( c ) fuller ' s method

    本研究選取c 、 d 、 e 、 r群及2型鏈球菌標準菌株，以高壓法提取抗原建立了檢測豬鏈球菌病血清抗體的dot - ppa - elisa方法。
  16. The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively

    豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。
  17. Thirteen putative epitopes showing characteristics of antigenic epitope were found from the analysis information. using pcr, the nucleotide acid fragments encoding these putative epitopes were amplified, then cloned into the expression vector miske. the positive recombinant phage displying the epitopes were found out by using pcr, sequencing and the determination of phage plaque titer

    運用goldenkey分子生物學軟體對prrsvbj - 4結構蛋白的抗原表位及其二級結構進行了分析和比較，從中篩選13段顯示表位特徵的氨基酸殘基序列，用pcr技術擴增相應的核苷酸片段，將其插入到噬菌體表達載體m13ke ，結果預測的13個表位可在噬菌體表面得以展示。
  18. The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis

    取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。
  19. In this experiment, hbsag ( adw subtype ) was introduced into lettuce ( lactuca sativa ) with the agrobacterium transformation system and biolistic method. we hope to obtain the transgenic lettue with hbsag so that it can be used as edible vaccine

    本實驗採用農桿菌介導法和基因槍介導法，研究了乙肝表面抗原基因（ hbsag ）導入可生食的萵苣（生菜） （ lactucasativa ）的核基因組和葉綠體基因組，並期望獲得轉基因萵苣植株，從而建立以萵苣為生物反應器生產可食用的乙肝疫苗。
  20. Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii, the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa

    3 mg _ 7重組噬菌體抗體庫的淘篩用m13ko7輔助噬菌體感染轉化菌，以挽救出噬菌體形式的抗體庫；用高表達mg _ 7抗原的胃癌細胞系kato對抗體庫進行三輪淘篩；用elisa篩檢呈現有mg _ 7scfv的噬菌體單克隆。
分享友人
分享到 Line

分享到臉書