表型反轉 的英文怎麼說
中文拼音 [biǎoxíngfǎnzhuǎn]
表型反轉
英文
phenotypic reversion-
Study of " ph memory " and activity water ( aw ) in processing of immobilization indicates that former factor has much effect on transformation and the latter just has little effect. permeabilization treating of baker ' s cell with alcohol and ultrasonic leads to lower transformation rate, which illustrates that permeabilization procedure has negative role on biotransformation with whole cell as biocatalyst in organic solvent. in simple dynamics research amount of biocatalysu concentration of substrate and reaction course are considered and all of them have remarkable effect on transformation
Ph記憶、水活度及通透性處理的實驗結果表明， ph記憶對轉化有明顯的影響，水活度對模型反應的影響不大；作為完整細胞生物催化，細胞的通透性可能是傳質阻力的重要組成部分，利用不同濃度的乙醇和超聲波對細胞進行處理，結果表明，在有機相完整細胞生物催化中，通透性處理對轉化產生的是負面影響。G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。Here we found g proteins also function in leaf, silique development and the yield of pollen microspore. we observed several traits or characters in the offsprings of gpal, agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type, whereas the lamina length, petiole length and rosette diameter is smaller than the wild type, the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type, and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines
實驗中我們跟蹤觀察了多代異三聚體g蛋白a亞基超表達轉基因植株及a ， p亞基缺失突變體的表型特徵，發現突變體的葉片寬度大於對應的野生型，葉片長度，葉柄長度及蓮座直徑小於野生型，而超表達植株的上述某些特徵與突變體相反; gp時突變體的長角果長度，花梗柄部長度大於野生型，而超表達ga植株種英則略小於對照; gpal突變體長角果尖端未出現咭乙i突變體的特徵: gpal ，口gbl突變體花粉生成量大於野生型，而超表達ga植株的花粉生成量則略小於對照。This thesis was divided into eight chapters, and the main results and innovations obtained here can be summarized as follows : ( 1 ) the physics model of tsrs in frequency conversion crystals has been built up firstly. based on quantum - mechanical viewpoints and the following physical processes and parameters : the two - photo interaction of light with matter, paraxial diffraction of stokes, langevin noise sources, reflection at the faces and the edges of crystals, gain coefficient, beam aperture, pulse width and fluence of 3, the physics model of tsrs in kdp and kdp crystals acting as high - fluence frequency convector and the paraxial operator maxwell - bloch - langevin equations have been built up
全文共分八章，取得的主要成果及創新點如下： （ 1 ）首次建立了諧波轉換晶體的tsrs物理模型本文根據量子力學原理，在考慮如下物理過程和參量的基礎上：光與物質的雙光子相互作用； stokes光的傍軸衍射； langevin （郎茲萬）噪聲源；晶體表面反射和端面反射；增益系數、光束口徑、脈寬和三倍頻光能量密度，推導出高通量激光在kdp和kd ~ * p諧波轉換晶體中的tsrs物理模型和空間上的近軸算符maxwell - bloch - langevin方程組。Driven by the cauliflower mosaic virus ( camv ) 35s promoter, the er - shsp over - expressed constitutively. the growth and the phenotype of transgenic plants can be used for researching the function of er - shsp in improving tomato ' s cold resistance and the er - shsp chaperones function in vivo. after degested by kpnl and xbal enzymes from the pbs - er plasmid, the gene er - shsp - lehsp21. 3 was inserted into the prokii vector to construct an eukaryotic expressing vector
利用基因工程方法，將內質網小分子熱激蛋白基因（ endoplasmicreticulum - locatedsmallheatshockproteingene ， er - shspgene ） - lehsp21 . 3導入到番茄體內，使之在植物體中組成性表達（ constitutiveexpression ）內質網小分子熱激蛋白（ er - shsp ） ，觀察轉基因番茄在低溫條件下的生長和表型反映，研究er - shsp在提高植物耐寒性中的作用，同時為體內研究er - shsp的分子伴侶機制提供依據。To get in vivo evidences that apoplast calmodulin con 1d regulate plant growth and development process, a chimeric secretion form of calmodulin binding peptide, which contains a signal peptide, a calmodulin binding domain and a c - myc epitope was constructed. the chimeric gene was introduced into arabidopsis. it was expected that the overexpression of this chimeric protein could be secreted into cell wall and bound to apoplast calmodulin, which could reduce the apoplast calmoduin concentration to make an apoplast camodulin " antisense " plant. by observing the potential phenotype change of apoplast calmodulin " antisense " plant, the in vivo function of apoplast calmodulin on plant growth and developmental process could be speculated
但這些多是採用生理學手段和藥理學方法而得出的體外（ invitro ）實驗結果，為了取得質外體cam在植物生長發育過程中發揮重要作用的invivo實驗證據，根據動物中的一些研究方法，本實驗設計並構建了帶有信號肽、 cam結合肽（ can小肽） 、 epitope （ c - myc ）融合基因的載體，並將融合基因通過真空滲入法轉入擬南芥，預期過表達的融合蛋白將會被分泌到細胞外並與質外體cam相結合，這樣就會抑制質外體cam的功能，從而可以構建質外體cam的「反義」植株，通過觀察質外體cam 「反義植株」的表型改變，就可以推斷質外體cam在植物生長發育過程中的功能。Insensitivity to eth ( in eir1 - 1 root and etr1 - 3 ) led to the impossibility of iaa transportion from shoot tip to shoot bottom or root, so that result in the diminishment of root gravitropism
這種表型上的差異表明：高水平的eth加強了根的正向重力性反應； eth信號轉導的阻斷引起根的正向重力性及莖的負向重力性減弱或消失。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。Abstract : plant responses to salt stress via a complex mechanism, including sensing and transducing the stress signal, activating the transcription factors and the corresponding metabolizing genes. since the whole mechanism is still unclear, this review emphasize the biochemical events during the plant adaptation to salt stress referring to an index of importance : the homeostasis in cytoplasm, the biosynthesis of osmolytes and the transport of water. most of these biochemical events were elucidated by study of halophyte and salt - sensitive mutations, also many important genes involved were cloned and used to generate stress - tolerance phenotypes in transgenic plants. on the other hand, about the molecular mechanism in signal transduction, the research of arabidopsis mutations and yeast functional complementation provided helpful traces but not full pathway
摘要植物對鹽脅迫的耐受反應是個復雜的過程，在分子水平上它包括對外界鹽信號的感應和傳遞，特異轉錄因子的激活和下游控制生理生化應答的效應基因的表達.在生化應答中，本文著重討論負責維持和重建離子平衡的膜轉運蛋白、滲調劑的生物合成和功能及水分控制.這些生理生化應答最終使得液泡中離子濃度升高和滲調劑在胞質中積累.近年來，通過對各種鹽生植物或鹽敏感突變株的研究，闡明了許多鹽應答的離子轉運途徑、水通道和物種特異的滲調劑代謝途徑，克隆了其相關基因並能在轉基因淡水植物中產生耐鹽表型;另一方面，在擬南芥突變體及利用酵母鹽敏感突變株功能互補篩選得到一些編碼信號傳遞蛋白的基因，這些都有助於闡明植物鹽脅迫應答的分子機制。Reverse malignant phenotypes of colonic carcinoma cells transfected with antisense gene to htrt
反義人端粒酶催化亞單位基因轉染對人結腸癌細胞惡性表型的逆轉作用Based on the analysis of gas - solid multiphase catalytic reaction, the chemical reaction model is established. when the reaction is controlled by the chemical dynamic, the reaction rate is introduced by consideration of the adsorption and desorption effects. when the reaction is controlled by mass transfer, the diffusion coefficient is established which can present the flow rate increases in small reynolds domain ; for the internal diffusion, the internal diffusion coefficient is derived, and then the internal diffusion is coupled with chemical reaction to represent the phenomenon that the diffusion and the chemical reaction occur cocurrently
本文在考慮催化轉化器載體內發生的傳熱傳質現象的基礎上，建立了催化轉化器的一維單孔道和三維多孔道傳熱傳質模型;在分析載體內以氣-固多相催化反應為特點的工作機理的基礎上，建立了催化轉化器的化學反應模型:當催化轉化處于化學動力學控制區時，引入了考慮吸附、表面反應和脫附的表面化學反應速率公式，當催化轉化處于質量輸運控制區時，引入了決定外擴散能力的擴散系數公式和和決定內擴散速率的內擴散系數公式，並與化學反應速率相耦合，得出由擴散過程決定的化學反應速率公式。It is found that the rates can induce gene switch in the positive feedback system, and can regulate the oscillation range in the positive - negative feedback system
結果表明，在正反饋模型中轉錄率能誘導基因開關轉換；在正負反饋模型中轉錄率能調節蛋白質濃度的振蕩幅度。552333x52 the most optimized media is decided, that is glucose 26g / l, mgsoa 0. 3g / l, nacl 2. 8g / l, yeast extraction 8g / l, peptide 15g / l, kjhbpo4 0. 3g / l. study of correlation between culturing time and transformation rate reflects that 30 hours is needed
探討了不同活化時間對模型反應的影響，結果表明，將生物催化劑培養30小時，進行有機相中轉化可以得到最高的轉化率。For surgery tools models, this dissertation presents an algorithm to transform models constructed in solid modeling system to the developed virtual system in which surgery tools models can be constructed conveniently, accurately and parameterizely. the constructed models can have high level third dimension effect. for slice - imagings, this dissertation gives an algorithm for rebuilding solid model from triangulated patch surface model and the rebuilding processing function as an important function in reverse engineering has been added to 3d - modeler system, which is a feature solid modeling system developed by cad lab of dut, so the reconstruted bone model can be further processed in the cad system
研究了實體幾何模型向三維表面模型的轉化，針對手術規劃及模擬教學系統中的醫療器械模型，提出了一種利用三維實體造型系統構建實體模型，然後轉化為本文虛擬系統所支持的表面模型的方法，能夠方便、精確和參數化地建立模型；針對斷層掃描圖象，給出了基於ct圖象反求技術進行實體幾何造型的主要步驟，以使骨骼模型能夠在本研究所開發的特徵造大連理工大學博士學位論文型系統3dm 。The strong shear cut and grinding force of hlgb horizontal stirring grinding machine is used to the surface of the moderate supersonic carbon and the fusion particles of the cao & sio2 in this paper. the result of xps indicated that the si - c bond is detected on the surface of the particles. the high melting point superfine sic and caco3 are produced on the surface of cao & sio2, and composite particles are formed
本論文利用hlgb臥式雙向旋轉攪拌球磨設備，通過正反轉攪拌齒及研磨珠工作時產生的強烈的剪切、研磨機械力持續作用於cao sio _ 2熔融顆粒及中超碳黑顆粒表面， xps檢測表明，顆粒表面有si - c及ca - co鍵生成，根據機械化學理論是由於機械力的作用，誘發利用熱能難以進行的機械化學反應，在cao sio _ 2熔融顆粒表面生成了sic及caco _ 3新物質，構成包覆型復合粒子。Theses abrators are suitable for surface treatment, derusting and surface erinforcing for all kinds of large, mediun and small sized casting pieces, forged pieces, and prooessed pieces, during shot cleaning, when the crawler rotates forward, the workpiece will roll : when the crawler rotates backward, the workpiece will unload itself
該系列拋丸機適用於各種中小型鑄件、鍛件及加工件的表面處理、除銹及拋丸強化，拋丸處理時履帶正轉使工作翻滾，履帶反轉工件自動卸料。The analytical solutions of the gain, dispersion and population difference in the steady state of an open ladder lasing without inversion ( lwi ) system are at first given when the phase fluctuation of the driving field is considered. then the effects of the driving field phase fluctuation on the gain, dispersion and population difference are analyzed by the numerical results from the analytical solutions. our research shows that the phase fluctuation of the driving field will results in a finite line width ; with the line width increasing, the gain decreases monotonously, however, variation of the dispersion isn ? t monotonous, it has a maximum value when the line width takes some value ; the line width varying can ? t lead to change lasing from without to with population inversion
給出了有驅動場位相漲落的開放的梯型無粒子數反轉激光（ lwi ）系統增益、色散和布居數差的定態解析解，進而用得到的數值計算結果分析了驅動場位相漲落對增益、色散和布居數差的影響.研究結果表明，驅動場位相漲落將導致有限線寬;隨著線寬的增加，系統增益單調地減小，但系統色散的變化並不是單調的，當線寬取某個值時，色散有極大值;線寬的變化不能導致無反轉激光向反轉激光的轉變The bic method generalized from ar model was adopted to determine the number of input neurons in grnn prediction model. the grnn was applied to single - step and multi - step ahead prediction of the vibration time series of a rotating machine, and its performance was compared with that of 3 - layers perceptrons network with error back propagation training algorithm ( bpnn ). it is indicated that the grnn is more appropriate for prediction of time series than the bpnn, and the performance of grnn is qualified even with sparse sample data
研究了基於廣義回歸神經網路（ grnn ）的大型旋轉機械振動狀態預測，提出了應用bic準則確定grnn預測模型輸入神經元數目的方法，將grnn用於大型機組振動峰?峰值時間序列的預測，與採用誤差反向傳播學習演算法的三層前饋感知器網路（ bpnn ）的預測結果對比表明， grnn的預測性能優于bpnn ，而且，即使樣本數據稀少，也能獲得滿意的預測結果。According to this process, the key technology which including the characteristic measurement of surface, the pretreatment of data getting by line scan, the character expression and fitting of curve and surface, the characteristic identify of surface and translation from the model of surface to cad
對實物反求過程中的各關鍵技術作了研究，包括：曲面的特徵測量、逐行掃描測量數據的預處理、曲線曲面的特徵表示及其擬合、曲面的特徵識別以及曲面模型向cad模型的轉化。For the evidence of the research, this paper puts forward the assumption of relation between earnings management and the reverse change in p / e ratio from a new visual angle, studies the earnings management taking use of four models, for example industry - scale total accruals model, extended - jones model etc. the empirical results find some evidence that the chinese listed companies with reverse change in p / e ratio have taken earnings management
接著為了實證的需要，本文將證券市場的評價指標市盈率引入，從一個新的視角市盈率反轉變動出發，提出了市盈率反轉變動與盈餘管理的研究假設。通過運用包括擴展的瓊斯模型在內的四個模型，對市盈率反轉變動上市公司的盈餘管理進行實證研究，分析了不同市盈率反轉變動上市公司市盈率反轉前後的盈餘和成長性表現。分享友人