In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的
片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的
片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口
蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。
The horses' hooves raised a cloud of dust.
馬
蹄揚起了一
片塵土。
An over abrasion of brake shoe and inconsequence breaking clearance is the main cause of invalidations for drum brake
對鼓式制動器而言,制動
蹄片磨損過薄以及制動間隙不合理是其制動失效的主要原因。
I look to hear the tromp of their boots any moment
我能看到他們的鐵
蹄可能隨時會踐踏這
片土地
Hom trade persia company was founded in tehran, iran in 2005 by a professional manager with over 20 years of experience in the automobile manufacturing and auto parts distribution industries products include clutch disk, clutch cover, brake pad, brake shoes, engine parts , shock absorbers we continuously search for manufacturers of high quality spare parts worldwide
該伊朗貿易公司,創建於2005年,在汽車配件經營行業已有20年的歷史,主營產品有離合器鋼
片,離合器蓋,剎車
片,剎車
蹄,發動機件,減震器,並向世界各地求購
The sounds of voices, of steps, and of horses hoofs tramping in the mud, the crackling firewood far and near, all melted into one fluctuating roar of sound
說話聲腳步聲和在泥濘中移步的馬
蹄的響聲近處和遠處的柴火的噼啪聲,融匯成一
片振蕩的嗡嗡聲。
When i came to the stile, i stopped a minute, looked round and listened, with an idea that a horse s hoofs might ring on the causeway again, and that a rider in a cloak, and a gytrash - like newfoundland dog, might be again apparent : i saw only the hedge and a pollard willow before me, rising up still and straight to meet the moonbeams ; i heard only the faintest waft of wind roaming fitful among the trees round thornfield, a mile distant ; and when i glanced down in the direction of the murmur, my eye, traversing the hall - front, caught a light kindling in a window : it reminded me that i was late, and i hurried on
我路過臺階時駐足
片刻,舉目四顧,並靜聽著。心想馬
蹄聲會再次在小路上回響,一位身披斗篷的騎手,一條蓋特拉西似的紐芬蘭狗會重新出現在眼前。但我只看到樹籬和面前一棵沒有枝梢的柳樹,靜靜地兀立著,迎接月亮的清輝我只聽到一陣微風,在一英里開外,繞著桑菲爾德府的樹林時起時落當我朝輕風拂拂的方向俯視時,我的目光掃過府樓正面,看到了一個窗戶里亮著燈光,提醒我時候已經不早。
Machine for cutting friction lining of brake shoe
制動
蹄摩擦
片切削機
In the darkness they flowed on like an unseen, gloomy river always in the same direction, with a buzz of whisper and talk and the thud of hoofs and rumble of wheels
在黑暗中,有一條看不見的黑? ?的大河,彷彿朝著一個方向平緩地流動。絮語聲和說話聲馬
蹄聲和車輪聲互相交織成一
片。
Each shoe is faced with a material, known as brake lining.
每個制動
蹄面上襯貼著一種物質,叫制動
片襯。
Water chestnut slices
馬
蹄片金針菇
Brake shoe automatic uster
蹄片自動調整裝置
Abstract : the paper analyses the forming process of eccentric shaft with cross - wedge rolling. the authors show the special forming method of eccentric shaft, take the brake - shoe shaft of ca141 truck as example and make an experiment on it. the research keeps up with standards of assembling and is put into production. the achievements have practical significance to extend the area of cross - wedge rolling forming to engineering use
文摘:分析了利用楔橫軋工藝成形偏心件的過程,給出了偏心件的特殊成形方法,並以ca141汽車剎車
蹄片軸為例進行了試驗,取得了滿意的結果,該研究成果對于開拓楔橫軋成形工藝應用的新領域有實際意義。
According to the abrasion of brake shoe, an abrasion indicator is designed which works based on the auto - interruption regulating arm
針對制動
蹄片的磨損,本論文設計了一種工作在自動間隙調整臂基礎上的制動
蹄片磨損傳感器。
Brake shoe grinder
制動蹄片磨削裝置