轉果糖酶 的英文怎麼說
中文拼音 [zhuǎnguǒtáng]
轉果糖酶
英文
transfructosylase-
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。For example, in glycolysis, glucose phosphate isomerase catalyzes the conversion of glucose 6 - phosphate to fructose 6 - phosphate
例如，在糖酵解過程中葡糖磷酸異構酶催化6 -磷酸葡糖轉化成為6 -磷酸果糖。A study on the relationships between acid invertase sucrose synthase and sucrose metabolism in red fuji apple fruit
蘋果果實蔗糖代謝與酸性轉化酶和蔗糖合酶關系的研究The stations e2 and 1 - 4 were located at the cold water mass area of the central yellow sea, which characterized by low temperature, high salinity and stable theromocline would generate a retention mechanism that promoted the formation of separate, self - supporting stocks of krill. 2 genetic diversity and differentiation of p. latifrons specimens of p. latifrons were collected from the east china sea and the south china sea. the zymogram phenotypes of aspartate aminotransferase ( e. c. 2. 6. 1. 1, aat ), alkaline phosphatase ( e. c. 3. 1. 3. 1, alp ), a - amylase ( a - amy ), r - amylase ( r - amy ), esterase ( est ), lactate dehydrogenase ( ldh ), raalate dehydrogenase ( mdh ), malic enzyme ( me ), and phosphoglweoisomerase ( pgi ) were scored
（二）寬額假磷蝦遺傳多樣性和遺傳分化研究1 .本文對東海外海和南海2個站位寬額假磷蝦群體進行了分析，在檢測的9個酶系統中，共檢測到11個酶位點:天冬氨酸轉氨酶（ l個位點， 2個等位基因） ，堿性磷酸酶（ 2個位點， a加了和a加2各有2個等位基因） ， r澱粉酶（ l個位點， 2個等位基因） ，醋酶（ 2個位點， es巧和est7各有2個等位基因） ，蘋果酸脫氫酶（ l個位點， 3個等位基因） ，蘋果酸酶（ l個位點， 2個等位基因） ，乳酸脫氫酶（ l個位點， 4個等位基因） ，磷酸葡萄糖轉氨酶（ l個位點， 3個等位基因） ; a澱粉酶為單態。The a - transglucosidase was selectively modified by pcmb, me, edc, clac, acetyl acetone and nbs, and changes in the activities of the enzyme have been detected. the reaction of a - transglucosidase with pcmb, me, edc and clac resulted in a strong inhibition of the enzyme activities which decreased with the increase of modifier concentration. the acetyl acetone and nbs were found without inhibition effect
酚丙酮、小涅代唬拍酷亞胺剛b引等化學修飾劑對a葡萄糖轉苦酶的幾種氨基酸殘基進行選擇修飾，並測定其酶活力變化，結果表明： pcmb 、 me 、 edc 、 ciac能顯著抑制酶的活性，活力的下降與修飾劑的濃度有關，乙酚丙酮、 nbs白巾飾對酶的奪製作用不明顯。Fos contain mixture of gf2, gf3 and gf4 sugars ( where g = glucose molecule and f = fructose molecule ) and a dp ( “ degree of polymerization ” ) of 3 - 5 ( “ neosugar ” ), are not naturally - occurring but are enzymatically synthetized from sucrose by action of an enzyme from the fungus aspergillus niger
詳細說明：是以蔗糖做底物，採用呋喃果糖苷酶轉果糖基作用，在蔗糖分子上以（ 1 2 ）糖苷鍵上與1 - 3個果糖分子結合，形成的蔗果三糖（ gf2 ） 、蔗果四糖（ gf3 ） 、蔗果五糖（ gf4 ）屬于果糖和蔗糖構成的直鏈雜低聚糖，在形成的產物中還有果糖、葡萄糖和未反應完全的底物蔗糖，採用色譜法除去單糖和雙糖制得高純度的低聚果糖。This contributes to make clearly position and function of - glc in forming course of tea aroma, substantiate the theory that the aroma of tea forms and also establish the foundation that utilize genetic engineering techniques to improve the cultivar of tea plant, quality of tea and insect - resistance and disease - resistance afterwards. in the paper, the complete cdna squence of - glc of tea was cloned in the tea leaf at the first time
因此，嘗試從茶葉中獲取-葡萄糖苷酶基因的cdna序列，將有助於進一步搞清-葡萄糖苷酶在茶葉花果香氣形成中的地位和作用，充實茶葉香氣形成的理論，也為以後利用基因工程手段改良茶樹品種，提高茶葉品質、抗病蟲能力以及該酶的轉化、調控的研究奠定基礎。Study on characteristics and thermostability of immobilized fructosyltransferase
固定化果糖基轉移酶的理化性質及穩定性研究I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]
從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。This study was to investigate the effects of sulfur dioxide inhalation at different concentrations on some glutathione - related enzymes such as glutathione s - transferase ( gst ), glucose 6 - phosphate dehydrogenase ( g6pd ) and glutathione reductase ( gred ) in brain, lung, heart, liver, kidney and spleen of mice by the technology of biochemical toxicology. the results were showed as follows, so2 exposure at different concentrations caused the changes of glutathione redox system. moreover, the activities of antioxidative enzymes and the contents of reduced glutathione ( gsh ) were decreased significantly in different tissues at higher concentrations of soa
本研究利用生化毒理學技術研究了不同濃度二氧化硫吸入（ 22 2mg m ~ 3 ， 64 3mg m ~ 3 ， 148 23mg m ~ 3 ）對純系昆明小鼠腦、肺、心、肝、腎、脾六種組織的谷胱甘肽還原酶（ glutathionereductase ， gred ） 、谷胱甘肽硫轉移酶（ glutathiones - transferase ， gst ）和葡萄糖- 6 -磷酸脫氫酶（ glucose6 - phosphmedehydrogenase ， g6pd ）活性的影響，結果表明so _ 2吸入使小鼠不同組織的谷胱甘肽氧化還原系統發生了改變，表現為隨著so _ 2吸入濃度的增加，該系統中的抗氧化酶活性的顯著變化和抗氧化物質水平的顯著降低，且存在著組織差異性。A. niger m - l which was screened in our laboratory produced a strongly a - transglucosidase. in this paper, studies on the fermentation conditions, purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation. the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis, the suitable main medium was achieved : which contained 4 % a, 2 % b and 1 % g ; the a. niger m - l was inoculated into 100ml medium in flask, shaking in 33 c at 140r / min for four days, with initial ph6. 5 and 6 % inocula volume ; adding 0. 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation, the a - transglucosidase activity amounted to 296. 05u / ml
本研究以黑麴黴m - 1為出發菌株，對其-葡萄糖轉苷酶的產酶影響因素、純化、酶學性質以及必需基團進行系統的研究，結果如下：通過對影響黑麴黴m - 1產-葡萄糖轉苷酶的單因素分析，得液態發酵生產-葡萄糖轉苷酶的最適產酶條件為： 4 a ， 2 b和1 g ；在33 ，起始ph值為6 . 5 ，轉速為140r min ，接種量為6 ，裝液量100ml條件下，發酵4 . 0d ，酶活力達296 . 05u ml ，添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷對產酶的誘導作用最大。From the result of electrophoresis, it known that the different components of the enzyme system were expressed cooperatively. in order to study the essence of cellualase induction of different carbon sources, the extracellular, plasm - membrane - bound and intracellular cellulases were made to transform different soluble inducers, and the productions were analyzed by gc chromatogram. the results supported the assumption that cellobiose acted as the direct inducer or the metabolic analogue, b - gentiobiose from cellobiose acted as the true inducer through different metabolism ways in different strains
制備細胞膜外、細胞膜、細胞內纖維素酶，用定位於這三部位的纖維素酶分別轉化底物，然後進行氣相色譜定性分析，從而探討了不同碳源之間的誘導本質，結果認為不可溶的胞外纖維素以纖維二糖為橋梁，遵循不同的代謝途徑，直接或間接地誘導了兩株不同真菌纖維素酶的合成。Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method
首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。The purified production was cloned into pmd18 - t vector. the cloned plasmids were transformed into jm109. the specific recombinant plasimid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of ibdv a fragment at right orientation
經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pmd18 - t載體中，再轉化到jm109感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆，結果表明，得到的陽性重組子中含有a節段全長基因，插入到載體中的方向正確。分享友人