轉錄弱化 的英文怎麼說
中文拼音 [zhuǎnlùruòhuà]
轉錄弱化
英文
transcriptional attenuation-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。It ' s significant to further the study of the environment evolvement and forming mechanism, and this helps to open out its ecological essential, protect and develop the oasis, lacated in the cross belt which converge northeaster and northwester, yutian oasis was threatened by the ilowing sand, and has the bad nature conditions the main purpose of the paper is applying fractal theory to research on the change of transitional belt between oasis and desert, which is a typical ecosystem in arid zone
而荒漠與綠洲之間的植被帶即過渡帶則最為敏感、完整地記錄了這兩大生態系統之間的相互作用與相互轉化，深入研究這一特殊地帶的環境演變過程、特徵及形成機制對于揭示其生態學實質、維護和發展綠洲具有重要的實踐意義。於田綠洲位於東北風與西北風的交匯地帶，受到流沙侵蝕的危害很嚴重，自然條件非常惡劣，生態環境異常脆弱，人民的生存環境受到了很大的威脅。
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