選用基因型 的英文怎麼說
中文拼音 [xuǎnyòngjīyīnxíng]
選用基因型
英文
selected genotype-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。Two spider silk protein genes ( avfl and avf2 ) screened from the library are characterized in a lot of repetitive motifs, a high guanosines or cytidines content, a strong preference for adenosine or thymidine in the third position of a codon and rich residues of glycines or alanines in the proteins translated
試驗結果，該文庫容量為4 . 9 10 ~ 6 。從文庫中篩選到avf1和avf2蛛絲蛋白新基因，具有典型重復序列多， g c含量高，密碼子第三個堿基偏愛使用a t及編碼蛋白中含有大量gly和ala殘基等特點。And also expounding that the first - fight - time can give explanation to the advanced techniques, the estimating ranges of first - fight - time in alcc estimating are expanded. the type - changed index and type - difference are selected as dummy variables. it has discussed mearsursion of type - changed index, and three criterions and three methods to set models including the dummy variable are got
說明性變量選取：說明性變量的選取基於費用因子分析，特別討論了首飛時間對費用預測的影響，論述了首飛時間能對技術先進性作出說明，拓寬其在費用預測中的作用，選取改型指數虛設變量，研究了改型的量化方法，提出了判別準則和實現方法。According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。Functional genomics uses as its starting point the isolated gene whose function is to be determined ， and then selects a model organism in which a homolog of that gene exists
功能基因組用功能不明的分離基因作為起始點，然後選擇具有該同源基因的生物模型。In ranking the indicator system of affecting cultivated land being selected into prime farmland has been built firstly, which is composed of 18 indicators involved in quality, location, policy attribute and administrative intervention of cultivated land. then based on the character of ranking, the idea of combination decision has been brought forward, ranking cultivated land synthetically with three ranking models including a model by similarity to ideal point, fuzzy optimization model and attribute hierarchy model. in three models the weights are based on hierarchy analysis and entropy weights, considering not only subjective partiality but also the intrinsic information of decision objects, which make the ranking results more scientific, reasonable and credible
在耕地綜合排序中首先建立了耕地入選基本農田的決策指標體系，由耕地質量狀況、區位條件、政策屬性以及行政干預4大決策因素共18個決策指標構成；然後根據排序問題的特點，提出了「組合決策法」的思路，並採用逼近於理想點的排序模型（ topsis ） 、模糊優選模型（ fom ）和屬性層次模型（ ahm ）三種排序方法對耕地進行綜合排序，每種排序模型中均採用基於層次分析法和熵權系數法確定的綜合權重，既考慮決策者的主觀偏好，又充分利用決策對象的固有信息，使排序結果更為科學、合理、可靠。In order to benefit from this new technique without any dangers, on the basis of the arguable contents, the paper put forward a series of principals with the knowledge of systematical philosophy, mathematics and ecological. agriculture : welfare principal, invariable principal of gene, non - spread principal of gene, equal principal of substance and informed perfectible principal. and then we establish a model of evaluating the social effects of gmo
為了從這種新技術成果中獲得好處，同時又不會帶來風險，本文以爭論的內容為基礎，從轉基因作物能比傳統農作物帶來更多的社會效益的角度出發，運用系統哲學、生態農業和數學的一些知識，提出了轉基因作物研究和推廣中遵循的一系列原則：福利原則、基因穩定性原則、基因不擴散原則、實質等同性原則、以及知情選擇原則，並進而建立起評價具體的轉基因作物的社會效益的模型。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。In accordance with the specific features of the objects under model observation, the models were estimated by ordered - probit and 0 - 1 probit respectively for the consumer acceptance and willingness - to - buy will concerning gm foods
根據模型考察對象的具體特徵，研究中分別採用有序多項選擇模型（ ordered - probit ）和二元選擇模型（ 0 - 1probit ）估計了消費者對轉基因食品的接受程度和購買願意。Therefore, a model of three sectors, respectively referring to domestic sector, manufacturing export sector and primary product export sector, has been established here to measure the technology spillover effects of export on domestic sector. finally, based on a broader framework, this dissertation went on to investigate the relationship between openness and china ' s economic growth, while the result shows that though there exists a notable dispute about how to measure the degree of china ' s openness, the index of trade dependence still maintains the better one to reflect china ' s economic openness. in the meanwhile, impulse response function ( irf ) method and forecasting errors variance decomposition ( fevd ) method, both of which are based on the vector auto - regression ( var ) system, are used here to investigate the dynamic relationship between openness and china ' s economic growth
與傳統理論不同，新增長理論和新貿易理論都強調技術進步的作用，因此本文構建了一個三部門的技術外溢效應模型（國內部門、工業製成品出口部門以及初級產品出口部門） ，考察了工業製成品出口和初級產品出口對國內非出口部門不同的技術外溢效應；第四，從更加廣闊的視野就貿易開放度與中國經濟增長問題進行研究，有關貿易開放度如何度量一直是存在較大爭議的問題，本文首先對該類研究文獻進行了較為詳盡的述評，然後運用生產函數方法對所選取的5個貿易開放度度量指標進行了檢驗，結果發現盡管一些已有研究認為外貿依存度無法真實度量一國經濟開放水平，但是本文研究結果表明外貿依存度仍是度量我國貿易開放度的較好指標，進一步採用基於var系統的脈沖響應函數法以及預測誤差方法分解法對貿易開放促進經濟增長的作用進行了動態刻畫。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。In this study, sd rats were used to establish the animal model of brain injury induced by repeated + gz exposure and suppression subtractive hybridization technique was adopted to screen the differentially expressed genes in rat brains of + gz exposure group. the aim of the study was to obtain preliminary experimental data for the molecular mechanisms of the brain injury
本研究利用大鼠重復+ 10gz暴露引發腦損傷的動物模型，觀察腦的病理學改變；應用抑制性消減雜交技術篩選+ gz重復暴露大鼠腦的差異表達基因，旨在初步探討+ gz重復暴露致腦損傷的分子機制。Studies of genes related to heart development in drosophila contribute to reveal the mechanisms of human heart development and the congenital heart diseases. to clone and identify new genes that control the heart development, by a way of chemical mutagen, ems, we have established 1, 200 balanced - lethal lines on chromosome 2 and 3. with the screening the 330 stocks with immunochemical method using heart - specific antibody, mab. no. 3, we detected 60 lethal lines showing heart mutant phynotype
為了克隆和鑒定控制心臟發育的新基因，本研究利用化學誘變劑甲磺酸乙酯大規模地誘變果蠅，並且建立了1200個第二和第三染色體的平衡致死系，利用心臟組織特異抗體mab . no . 3對其中330個品系進行免疫化學方法篩選，觀察到有60個致死系表現出心臟突變表型， 20個品系的心臟突變表型有待進一步證實。A method of fuzzy optimization design based on genetic algorithm is presented as a new method of parameter optimization design for dc double closed loop speed adjusting system. the method covers three steps. firstly, speed overshoot rate and settling time are chosen as performance indice according to the demand of engineering. these indice are normalized by using fuzzy membership function and then weighted to form objective function of optimization model of the system. secondly, the dynamic response curve of the system with corresponding parameters and peoformance indice are obtained by computerized numerical calculation and simulation. finally, parameters of engineering design are expanded as searching space ; and parameters of speed regulator and current regulator are taken as genes in chromosome. these genes in searching space are optimized to get best solution by way of genetic algorithm. as shown by experimental results, the parameters designed by this method are capable of significantly improving performance indice of the system, which proves that it is a practical and effective method
提出一種基於遺傳演算法的直流雙閉環調速系統參數優化設計方法.根據工程技術的要求，選用速度超調量和過渡時間作為參數優化性能指標.將該指標用模糊隸屬度函數歸一化，再加權平均形成系統優化模型的目標函數.採用計算機數值計算方法，通過模擬獲得系統對應參數的動態響應曲線及其性能指標.最後以工程設計的參數為搜索范圍，以速度調節器和電流調節器的參數為染色體中的基因，通過遺傳演算法在搜索范圍中優化這些基因，獲得優化解.實驗結果表明，所設計的參數能使系統性能指標有顯著提高In order to select male nucleo - sterile new genotype, a tentative idea was put forward for the nucleo - male sterility to attach a tps ( thermo - photoperiod sensitivity ) and a selection strategy of combination of selection and identification, at the same time, the spring and summer sowing method were used to provide different environment conditions of appraising sterility and tps. the results indicated that ( 1 ) sterility could be appraised under the spring sowing environment and tps could be appraised under summer sowing environment. ( 2 ) under spring sowing environment, sterility could be selected, but not maintained. thereby, lines selected could only be selected as recorded selection method in the experiment. ( 3 ) and then, selection was carried out from spring sowing line selected into summer sowing in same line with tps to select plants. these plants through the intercrossing selection had been combined with sterility and tps. in this way, a new selection protocol for selection sterile line with tps was formed. it mainly involves the spring and summer sowing method, recorded selection method and the intercrossing selection method
為了選育新類型玉米雄性核不育系，提出了為玉米核不育性添加溫光敏感性的設想和選擇與鑒定相結合的策略，同時應用分期播種的方法為作物提供不同的生長和發育的環境條件，以鑒定玉米雄穗的育性變化和對不同環境條件溫光的反應.研究結果表明，春播環境下可鑒定和選擇玉米的不育性，夏播環境下可鑒定和選擇其溫光敏感性.針對玉米核不育性難以找到保持系的特性，結合兩種播期選擇兩種性狀.但春播環境下選擇的不育性群體難以通過選擇單株來保持其不育性，為記錄性群體選擇.通過從春播入選的雜合不育性優良株系群體轉移到其對應的夏播溫光敏感性入選的同一優良群體中進行優良單株選擇，能逐漸使不育性和溫光敏感性相結合而選育出純合溫光敏不育系.這種新的選擇程序主要包括應用分期播種法、記錄性選擇法和春夏兩季交叉式選擇法，使含有不育基因的可育株系逐步累積不育基因並增加了溫光敏感性而育成玉米溫光敏不育系In this paper, river water quality prediction is integrated with water pollution control measures. two parameters, water environmental capacity and pollution index are selected for pollution control measures. the objective is achieved after the development of simulation model using one - dimensional advection dispersion equation. the model algorithm and computer program is an improvement over the existing water quality models, since the model solution involves four point implicit upwind schemes for water quality prediction and pollution control measures at each grid point. model simulation results the assessment of water environmental capacity that yield the acceptable and realistic pollutants concentration in order to maintain water quality objectives. the pollution and overall pollution index of the river is suggested for integrating number of contaminants variables into one index. study provides the mathematical and scientific procedure for water quality management. the new approach is helpful for the water pollution control and to study the impacts of waste effluents on the river system for strategic planning purposes
本文將水質預測及水污染控制措施有機地結合，選取水環境容量和污染指數作為水污染控制的參數.這樣，一維對流-擴散水質方程的求解除就是實現本研究目的的關鍵.本研究採用的模型在現有的水質模型基礎上有所改進，因為其採用四點隱格式對水質進行預測，推求可接納污染物的環境容量值及為保證水質而限定的污染物濃度值，從而制定相應的水污染控制措施.為整體考慮各種污染物的情況，建議採用河流的污染指數進而推求綜合污染指數.總之，本研究為水質保護提供了科學的計算方法，該法對水污染控制及污水對河道水質的影響是實用有效的The study was undertaken to isolate fe ( ii ) - transporter cdna and related binding protein cdna under fe - deficiency stress from a fe - deficiency root cdna expression library of malus xiaojinensis by screening library using maize fe ( ii ) - transporter cdna and wheat tamre - bp cdna as a probe. the main results as follows : 1 out of approximately 120000 plaques, four positive cdna clones encoding fe ( ii ) - transporter proteins, designated pftl -
本試驗以蘋果屬小金海棠（ malusxiaojinensischengetjiang ）為試材，利用玉米fe （ ）轉運蛋白基因片段以及小麥金屬反應元件結合蛋白tamre - bpcdna為探針，篩選小金海棠缺鐵根cdna表達文庫，目的在於克隆蘋果鐵高效基因型小金海棠的fe （ ）轉運蛋白基因以及與缺鐵脅迫相關的結合蛋白基因。The basic principle of natural gamma - ray log is stated, the developing background, developing ways and developing situation of natural gamma - ray tools are introduced. the researching task of the paper is presented through analyzing the using situation and questions exsisted in inner natural gamma - ray tools, the researching work is started from three aspects, they are logging tool development, reliability design and reliability assuring methods, and the data processing methods, in the course of logging tool development, instrument indexes are presented based on the compatible property of sookbps telemetry system and environmental property, the analog measuring chanel and the interface circuit which realizing the compatible performance are designed according to the instrument mdexes. the detecto * design. the plateau property testing of the detector and the analysis of it ' s affecting factors are stated, the measuring property of the tool is discused, a new type of single chip microcomputer is selected when designing the interface circuit, and the laboratory experiments has fulfiled conmunieating standard signals between the interface circuit, the universal interface unit of sookbps telemetry system and also 500kbps telemetry system
本文首先概要介紹了石油測井的基本概念、方法、條件、最新進展、以及應採取的研發對策，論述了自然伽瑪測井的基本原理，介紹了自然伽瑪測井儀的發展背景、發展歷程和發展現狀，通過分析國內自然伽瑪測井儀的使用情況和存在的問題，提出了本文的研究任務。研製工作從測井儀研製、可靠性設計與可靠性保障技術、數據處理方法研究三個方面展開，在測井儀研製過程中，根據500kbps遙傳系統要求的配接性能和使用環境特徵，提出了主要儀器指標，並根據這些指標，設計了儀器模擬測量通道和實現這一配接性能的介面電路；論述了探測器的設計、坪特性影響因素分析及其測試，探討了儀器的測量性能；在設計介面電路時選用了新型單片機晶元，並與500kbps遙傳通用介面單元rtu 、 500kbpa遙傳系統實現了室內配接。The paper summarized theories to interpret rural residents " income difference and math methods to measure income difference, which laid theory base for further analysis and provided empirical materials, it studied general income difference of rural residents by gini coefficient, theil index, decomposed gini coefficient according to income sources, ten equally separated methods and econometric models, and measured and analyzed income difference and its determinants of rural residents between regions and in liaoning province and neimenggu municipality
本文歸納了中國農村居民收入差距理論和收入差距的數學描述，為進一步分析奠定了理論基礎和提供了實證素材。本文選用基尼系數、泰爾指數、按收入來源分解基尼系數、十等分組法和計量經濟模型研究了中國農村居民總體收入差距情況，測度並分析了中國農村居民地區間收入差距、遼寧省和內蒙古自治區農村居民的收入差距狀況以及影響收入差距的因素。By gene fusion and prokaryotic expression, we purified a pea actin isoform ( peac1 ), his - tagged peac1, his - tagged gfp and his - tagged peac1 - gfp from inclusion body. after filtrating a series of induction condition, we expressed and purified his - tagged peac1 with soluble form in a large amount
利用基因融合技術，原核表達並從包涵體中純化了豌豆肌動蛋白異型體peac1 、 his - taggedpeac1 、 his - taggedgfp以及his - taggedpeac1 - gfp ，並通過誘導條件的篩選達到了可溶性表達與大量純化his - taggedpeac1 - gfp的目的。分享友人