酵母蛋白酶 的英文怎麼說

中文拼音 [jiàomǔdànbái]
酵母蛋白酶 英文
endotrypsin
  • 酵 : 動詞（發酵） ferment; leaven
  • 母 : Ⅰ名詞1 （母親） mother 2 （泛指女性長輩） one s elderly female relatives 3 （配套的兩件東西里的凹...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. The effect of adding neutral protease, papain and - glucanase on yeast autolysis were studied

    摘要研究了外加中性蛋白酶、木瓜蛋白酶和-卜葡聚糖酶對酵母自溶的影響。
  2. The growth of f - 16 and the production of enzyme were affected by microbial medium, including c source, n source, mineral, initial ph of medium, rotating rate, culture time and culture temperature. the results showed that the optimal n sources were peptone, corn steep liquor and yeast extract ; the optimal c sources were sucrose, glucose and maltose ; the optimal minerals were mgso4 7h2o, khpcu and cuso4 5h2o

    實驗表明，氮源中蛋白腖、玉米漿、酵母粉比較好；碳源中蔗糖、葡萄糖、麥芽糖這三種糖對產酶和生物量提高效果顯著；無機鹽中mgso _ 4 ? 7h _ 2o ， k _ 2hpo _ 4 ， cuso _ 4 ? 5h _ 2o對產酯酶的酶活及其反應后所得水解液的光學純度有較好的作用。
  3. The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

    進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。
  4. 5, histone acetyltransferase gene ygcns and histone deacetylase gene yrpd3 were cloned and expressed, which has laid down a foundation for studying chromosome remodeling in vitro

    5 ，我們克隆並純化了酵母中的組蛋白乙酞基轉移酶基因gcns和去乙酞化酶基因rpd3 。
  5. When the hameoly - mph were pretreated with activators such as sds, trypsin and zymosan, their po acti - vities increased significantly, reaching about twice of the untreated. this indicated that the po activities exist in the hameolymoh of penaeus chinensis and penaeus

    結果表明，中國對蝦和南美白對蝦血清中都存在po ，主要以酚氧化酶原（ propo ）的形式存在，並且都可以被胰蛋白酶、 sds和酵母聚糖激活。
  6. Acid protease gave play to synergetic action in liquor fermentation manifested as dissolving the granules of fermentation materials, advancing microbial propagation, decomposing protein to produce flavoring materials, and degrading yeast tropina

    摘要酒用酸性蛋白酶在白酒釀造的發酵過程中起協同作用，具有溶解發酵原料的顆粒、促進微生物繁殖、分解蛋白質生成香味物質、降解酵母菌體蛋白等多種功能。
  7. The main factors including foam - positive proteins and yeast proteinase a that influence foam stability of unpasteurized beer and the mechanism of these proteins affecting the foam are introduced, then the strategy for foam improvement are indicated in this paper

    摘要簡要介紹了純生啤酒泡沫穩定性的主要影響因素泡沫陽性蛋白和酵母蛋白酶a對啤酒泡沫的影響及影響機理，並簡要介紹了改善純生啤酒泡沫的主要策略。
  8. When mms was added directly to s. cerevisiae s288c cell extracts immediately before primer addition, telomerase activity remained unchanged. thus, a direct influence of mms on trap assay does not seem to be involved in telomerase activation observed after drug treatment

    如果在加人引物之前將mms直接加在釀酒酵母5288c細胞蛋白提取物zh ，結果顯示端粒酶活性沒有變化，因此排除了mms對端粒酶的直接激活作用。
  9. Xylanase refers to a type of enzyme which can hydrolyze xylans into xylooligosaccharides and d - xylose. it broadly exists in microorganism and has wide commerical application in industrial processes, such as feed, paper, foodstuff, medicine and energy industries. the xylanase gene xynb encoding the native protein of xylanase from streptomyces olivaceoviridis a1 was cloned into pichia pastoris expression vector ppic9 a

    我們將來源於橄欖綠鏈黴菌a1 （ streptomycesolivaceoviridisa1 ）的木聚糖酶基因xynb的成熟蛋白編碼序列克隆到畢赤酵母表達載體ppic9中，轉化pichiapastorisgs115得到重組子，實現了木聚糖酶基因xynb的高效表達，表達產物能有效分泌且具有正常的生物學活性。
  10. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  11. To research on the gene and protein structure of the lipase from penicillium expansum pf898 ( designated pel ), improve its property of cold - activity, we have done the research as follows -

    本文報道了擴展青黴堿性脂肪酶（ pel ）基因在巴斯德畢赤酵母中的表達及利用蛋白質工程改造pel基因的研究工作。
  12. In this thesis, the hplap gene was cloned into the ppiczaa plasmid and induced expressed in the protease - deficient strain p. pastoris smd1 168. the clone that could secrete hplap with enzyme activity was selected

    本研究將hplap基岡兌隆到spiczoa質粒中並在畢赤酵母屍pastorjs蛋白酶缺陷菌株smdi168中誘導表達，獲得了有活性的分泌型hplap 。
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