重插入 的英文怎麼說

中文拼音 [zhòngchārù]
重插入 英文
reinsertion
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 插 : 動詞1. （把細長或薄的東西放進、擠入、刺進或穿入; 插上; 插進） stick in; insert 2. （中間加進去; 加進中間去） interpose; insert
  • 入 : Ⅰ動詞1 （進來或進去） enter 2 （參加） join; be admitted into; become a member of 3 （合乎） conf...
  • 插入 : insert; infix; run in; break in; patch; insertion; plug in; intercalate; intercalation; intromiss...
  1. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  2. The results showed that the f fragment, 728bp in length, could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment, about 4kb in length, is inferred to have repeat sequences around tn5 insertion site, in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica. to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals, the experiment with mice was carried out

    結果顯示， f片段長度為728bp ，與現有生物數據庫的blast比較分析，發現該序列僅有局部短於1oobp的區域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性，推測為一新基因； b片段長約4kb ，序列拼接結果推測靠近tn5插入位點部位有重復序列，對b片段tn5遠端的部分序列進行blast比較，發現它與小腸結腸炎耶爾森氏菌的強毒力島有一定的同源性。
  3. In this paper we studied the intercalation behavior of a new host matrix of x = l of zirconium proline - n - mtthyl phosphonate - phosphate ( a - zpmpp ), we successfully introduced butylamine heptylamine decanylamine ethanolamine diethylenetriamine ( 2e3a ) triethyleneteriamine ( 3e4a ) and tetrathyleneoctamine ( 4e5a ) guest molecules into a - zpmpp interlayer space. the intercalation complex were characterized by ir spectrum x - ray diffraction and tg - dsc thermal analysis, it has been found that a - zpmpp possess different intercalation behavior from a - zrp. because of the bulk of proline group, it affected the amount of guest molecule, mono - alkylamine form mono - molecule layer in the interlayer space, butylamine, decanylamine and ethanolamine form mono - layer and the carbon chain form 90 degree ordered assembly with the zirconium floor of a - zpmpp, every host molecule absorbed 0. 5 guest molecule

    本文報道首次以x ? l的層狀（脯氨酸十一甲基磷酸一磷酸氫）鉛（ a zpmpp ）為主體底物，成功地將客體分子：正丁胺、正慶胺、正癸胺、乙醇胺、二乙烯三股、三乙烯四胺、四乙烯五股插入層狀化合物a zpmpp的層間，通過紅外光譜（ ir ） 、 x射線衍射（ xrd ） 、熱重分析（ tg dsc ）等手段對插層復合物進行結構表徵，結果表明： x ? l的層狀（脯氨酸件一甲基磷酸一磷酸氫）鉛具有不同於無機磷酸結的插層性能，由於層間脯氨酸基團的體積較大，影響客體分子進入的數量，胺分子在層板間取單層排列。
  4. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  5. The recombinant expression plasmids prt - p450nor and pet - p450nor were constructed by inserting p450nor gene into the bamh i / hind iii site of the prokaryotic expression vector prset and pet28, then they were transformed into e. coli bl21

    本研究將已克隆的真菌細胞色素p450nor基因插入原核表達質粒載體prset和pet28的bamhi / hind位點，成功構建重組表達質粒prt - p450nor和pet - p450nor ，並轉化到e . colibl21 。
  6. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  7. In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate

    將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。
  8. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  9. Vbi ( vertical blanking interval ) is a part of the limited resource of television, in which various digital signals can be inserted

    場消隱期作為電視有限資源的一部分，在其空余資源中可插入多種形式的數字信號，其開發潛力正逐漸為人們所重視。
  10. The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent

    將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。
  11. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  12. The thesis tells surface analysis method including curvature analysis and curve depth analysis that we using in the skin restoring project. in chapter 2, it tells the definition of b - spline curve and surface and the fundamental algorithm - de boor algorithm for points of curve and surface. as to reconstruct surface from ct data, we discuss some basic algorithm and introduce the wrapping skin algorithm

    在第二章中，介紹了b樣條曲線曲面的定義及基本演算法- -求曲線曲面點的德布爾演算法；基於ct數據的曲面重構，討論了重構過程中所應用到的基本演算法（如節點插入及消去演算法、曲線的反算方法等） ，並介紹了曲面重構中常用的蒙皮法。
  13. The studied factors respectively are : length of soil nailing, insert deepness of piles, friction force of soil nailing interface, declination angle of soil nailing, horizontal spacing of soil nailing, rows of soil nailing, unit weight of soil, friction angle, unit cohesion, overload of slope, diameter of soil nailing

    這11個因素分別為：土釘（錨管）長度、土釘直徑、土釘界面摩擦力、土釘下傾角、土釘水平間距、土釘排數、土體重度、內摩擦角、粘聚力、坡頂超載、板樁插入深度。
  14. The following actions to the table are considered changes : insert, update, delete, index rebuild or defragmentation, and database restore or attach

    對表的以下操作可視為更改：插入、更新、刪除、索引重建或碎片整理以及數據庫還原或附加。
  15. The ignore dup key setting applies only to insert operations that occur after the index is created or rebuilt

    Ignore _ dup _ key設置僅適用於創建或重新生成索引后發生的插入操作。
  16. After linerization, the recombinant plasmid was transformed into pic hia pastoris by electroporation, which then were cultured in md plate free of histidine, from which the positive colones were propagated

    重組質粒線性化后，用電擊法將重組質粒轉化入巴氏畢赤酵母，在缺組氨酸的md板上篩選陽性菌落，然後用不同濃度的g418 ? ypd板篩選多拷貝插入單菌落。
  17. Intra - operative monitoring of cortically recorded somatosensory evoked potentials ( seps ) by peripheral nerve stimulation is of value during orthopaedic surgery and is the state - of - the - art in terms of non - invasiveness, versatility, time requirement, lateral discrimination, and ease of electrode placement

    通過外周神經刺激由皮質記錄的體感誘發電位的術中監測在骨科手術中具有重要價值，具有非侵襲性，多功能，需時，側方識別，容易電極插入等優點。
  18. Kool - stop dual compound insert pads and titanium hardware have been used to keep the total weight to 28 grams per pair

    庫爾一站式雙重復合插入墊和鈦的硬體已被用來保持總重量28克一雙。
  19. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  20. There are three lifting holes and one lifting bolt hole on thefront and rear of the machine bed for plugging lifting hooks or bars

    床身前後共有三個起重孔和一個起重螺栓孔，用以插入起重工具。
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