重組基因技術 的英文怎麼說

中文拼音 [zhòngzǔjīyīnjìshù]
重組基因技術 英文
recombinant dna technology
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 技 : 名詞（技能; 本領） skill; ability; trick; technique
  • 術 : 術名詞1. （技藝; 技術; 學術） art; skill; technique 2. （方法; 策略） method; tactics 3. （姓氏） a surname
  • 重組 : bpr
  1. According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified

    以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。
  2. The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a

    其次，通過氨基酸序列和結構的比較，確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能；然後，應用重組dna技術，互換tm - 2 ~ 2基因和tm - 2基因的對應結構域，構建嵌合基因，獲得嵌合蛋白表達的轉化體，驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。
  3. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  4. Recombinant dna techniques deeloped for cloning genes

    重組dna技術被應用於基因克隆
  5. Recombinant dna techniques developed for cloning genes

    重組dna技術被應用於基因克隆
  6. The modern technology give a chance for the pattern of transmitting knowledge with network, the course based on network have opened up it ' s way in china, our pursuer paid more attention to the mode, but in america, pursuer have studied the more width aspect, thereinto the investigation and design of the information in the course based on network is very important, for the design of the information decide the pattern how the student receive the information, which will effect the way of knowledge understanding and memory, and the way is related to the study pattern, so the design of the information in the course based on network is very important to the efficiency and effect. so the core of the paper is the investigation and design of the information in the course. i have the entropy from the information science as the analyse tool to analyse the information, these work tell us the efficient pattern to transmit information, based on the result, i design the net page, of course, study theory and the character of the net itself are also the factors i employ for design a good net page. then, links and navigation is constituted for students adapt to the course based on network

    當今技術的發展給知識以網路為媒介來傳播的學習方式帶來前所未有的機遇，網路課程在國內迅速開展起來，但是國內研究者的注意力主要集中在對網路課程模式的探索上，放眼國外，他們的研究觸角已經涉及到網路課程比較細致的方面，尤其是對網路課程信息的研究構成了國外網路課程研究中的比較重要的一個方面，而網路課程中信息的設計是重要的，因為信息的組織設計是為了接受者能對信息進行有效的信息加工，信息的呈現模式影響著學習者對知識的理解和記憶方式，進而決定了學習者的學習模式，因此網路信息的設計在網路課程傳遞的效率和效果中就佔有很重要的位置。基於這個觀念，本文把網路信息的組織設計作為研究的重點，引入了信息科學作為研究的主要工具對網路中的信息作以量化分析研究，主要應用了信息科學中信息熵的公式進行推導，得到學習內容信息組織的基本模式，並充分利用網路自身特性和學習理論對知識信息進行細致的設計，此外還對鏈接和導航信息進行了設計，在網路課程的適應性方面作出了努力，把交互信息與輔助學習信息的分析設計與應用和對網路頁面信息的總體調節優化作為主體信息設計部分的補充，最終形成了網路信息組織設計的方案，力求創設一個能夠有效傳遞知識信息，減少網路自身弊病，並帶有一定適應性的網路學習環境，也使更多的網路課程的設計者關注網路信息這個因素。
  7. Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress

    最近的研究表明，基因轉移與重組技術在結縷草遺傳改良中具有巨大應用潛力；結縷草遺傳連鎖圖譜構建取得重要進展；基因克隆和基因資源研究正在展開。
  8. The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli

    從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e
  9. The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus

    本研究的目的在於以人的-干擾素基因（ ifn - ）作為目的基因，與鯉魚-肌動蛋白基因啟動子在體外重組，利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究，其結合了干擾素和基因工程育種抗草魚出血病病毒的優點，以期獲得對草魚出血病具有天然抗性的轉基因魚，並在此基礎上培育出草魚抗病新品系。
  10. By applying electrophysiological techniques on normal and gene knock - out mice, these researchers show that in the hippocampus, a brain area important for memory formation, bdnf is the only key protein required for the expression of late - phase long - term potentiation, a cellular model of long - term memory

    研究人員分別在正常和基因敲除小鼠身上以電生理技術作實驗，發現在它們的海馬區（腦部組成記憶的一個重要部位）內， bdnf是造成長期記憶的細胞機制- -晚期長時程增強- -表現時所需的唯一主要蛋白質。
  11. The thesis provides systematic study on theory and technique of design about adhm from main aspects after exploring the source and evolution of it : first, the thesis categorizes adhm and sum up its basic model from four distinct perspectives - - presenting forms of historical material, organizing construct of question, and requiring ability of answer and method of paying score. second, the thesis makes an exhaustive analysis to examine function of adhm from four levels - - memory - distinguish, comprehension - annotation, analysis - synthesis and evaluation - argument

    本文在對材料分析論證題出現的原因、演變作簡要探討后，著重從四個方面對材料分析論證題的命題理論與技術進行了系統研究：首先，從材料的表現形式、題問的組織結構、答題的能力要求和答案的賦分方式四個不同視角對材料分析論證題進行了分類，概括了材料分析論證題的基本模式，從而為整個研究奠定了基礎。
  12. By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature

    利用dna重組技術，將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb ， iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白，光密度掃描對表達產物進行初步定量，表明表達產物約占菌體總蛋白的14 。
  13. It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions, clearing aged cells and metabolic products, regulating immune responses and protecting against infection. in some pathological states such as psoriasis and contact dermatitis, a certain serum level of the antibody could inhibit the progression of the diseases, and is beneficial to the recovery from the diseases. after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies, meanwhile, experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) ", a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability, immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis, and recombinant antibody is under investigating

    抗角蛋白自身抗體（ akautoab ）是naa的重要組成部分，以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，並對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究，直接或間接地發現akautoab是機體正常免疫調節網路的組成部分，在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用；在某些病理情況下（如銀屑病、接觸性皮炎等） ，體內akautoab的組分和滴度會發生變化，而正常水平的akautoab則有利於限制病情的發展，促進損傷的修復。
  14. Research topics cover the basic and applied parasitology, including the major parasitic groups, pathogenic biology, host specificity, epidemiology, ecology, the control of parasitic infections, and biochemical and molecular parasitology, and ranging from host - parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture

    在整體和分子水平上，應用現代生物技術對危害人體和重要經濟動植物的主要寄生蟲（原蟲類、吸蟲類、絳蟲類、動物線蟲類、植物線蟲類及醫學昆蟲等）和由其引起的病蟲害進行研究，在病原生物學（包括生活史、媒介種類、基因組結構與表達調控等） 、流行病學、生態學，寄生動物與宿主間的相互作用關系等研究基礎上，實現對病原物的診斷、治療和控制。
  15. $ mx # ( 2bo2 ) ffr $ $ htet # $ nq9i & kk $ 4n8 $ $ 4ta scf mana $ ismffs whether s1eep deprivation cou1d induce neuron pro1iferation has not got definite conclusion so far. in the present research, we performed small pedestal s1eep deprivation method to remove rapid eye movement s1eep ( rems ). we observed the neuron pro1iferation and differential in immunohistochemistry and doub1e 1abeling iariunohistochemistry staining, and the expression of stem ce11 factor mrna in situ hybridization technique after s1eep deprivation in rat hippocampus

    本研究應用細胞增殖標記brdu cna免疫組織化學方法結合雙重標記兔疫組織化學技術，觀察睡眠剝奪對成熟大鼠腦內神經元增殖及分化的影響，應用原位雜交技術觀察了睡眠剝奪大鼠海馬神經細胞scf基因inrna表達的變化，對睡眠剝奪引起海馬神經細胞增殖的分子機製作了初步探討。
  16. Finally, the author puts forward the countermeasures and the suggestions in the development of the animal husbandry in jiuquan oasis, which are to confirm the rational amount of domestic animal loaded ; to protect the meadow resource and to develop the high - quality artificial meadow energeti cally ; to implement the virtual water strategy in order to alleviate the ecological environment pressure in jiuquan oasis ; to advance the industrialization process of animal husbandry energetically ; to process and utilize crops by product in the agricultural district ; to strengthen the construction of shelter forest of qilian mountains ; to organize the seasonal production of the animal husbandry ; to popularize the fatten poultries technology in the other land ; to strengthen the rational planning of the production of the animal husbandry ; to increase fund input ; to accelerate the basic construction of the animal husbandry and to prevent and cure the grassland which mouse hurt

    採用層次分析法對酒泉綠洲畜牧業發展的重要影響因素做了判斷。最後從確定合理的載畜量，大力保護草地資源和發展優質人工草地，實施虛擬水戰略以緩解酒泉綠洲的生態環境壓力，大力推進畜牧業產業化進程，對農區農作物副產品的加工利用、加強祁連山防護林建設、組織畜牧業季節性生產，推廣推廣肉禽異地育肥技術、加強畜牧業生產的合理規劃、增加資金投入，加快畜牧業基礎設施的建設以及草原鼠害的防治等十個方面提出了酒泉綠洲畜牧業發展的具體對策建議，以期望能對酒泉市的社會經濟發展提供科學的決策依據。
  17. Therefore, our cogonition on these experimental phenomena and laws makes a science base to further reveal the essence of eutrophication and provides an experimental base to implement bio - engeering technology of rebuilding and constructing waiter ecosystem, and also offters technologies for the microalgae controlled by the hydrodictyon reticulatum

    因此，通過對這些現象與規律的實驗認識為進一步揭示富營養化的成因奠定科學基礎;為實現對水生生態系統的生物重建、組建等生態工程技術提供實驗基礎;也為利用藻類控藻提供技術支持。
  18. The research interests of this group include : aborvirus diagnosis technology development and the interaction of aborvirus and mosquitoes, entomopathogenic bacteria and insecticidal gene resources, microbial genomics and comparative genomics, insecticidal proteins and their mode of action, construction of engineering strains with higher toxicity and wider active spectrum, production, standardization and the application of bio - pesticide and other microbial agents, resistance mechanism in target insects and the resistance management

    重點研究登革熱病毒、乙型腦炎病毒和西尼羅病毒的快速檢測及病毒與宿主的相互作用關系，蚊蟲病原微生物菌種及其基因資源，微生物基因組學和比較基因組學，殺蚊毒素蛋白特性和作用方式、殺蚊細菌的遺傳改良和工程菌株的構建，新型細菌殺蚊制劑的研製及野生型和重組微生物對環境的安全性評估等，發展新的生物防治技術，建立和完善以生物防治為主的蟲媒病毒媒介蚊蟲綜合防治體系。
  19. This paper describes several latest industrial microbial technologies in detail, which are the synthesis of the chiral diols by epoxide hydrolase from microbie, cofactors regeneration for redox with fdh, production of nano / micro wire by the phage display, metabolic network rebuilding for conventional fermentation and the application of the organic solvent tolerance and the metagenomics technology

    本文綜述了幾項最新的工業微生物技術，主要包括：微生物環氧化水解酶催化合成手性二醇、微生物甲酸脫氫酶用於再生氧化還原反應的輔因子、通過噬菌體展示技術得到納米級金屬絲、代謝網路改造和重建用於傳統發酵生產以及有機溶劑耐受菌和宏基因組技術的應用。
  20. We also work closely with colleagues from the faculty of science and the institute of chinese medicine of cuhk and participate actively in several major funded projects in applied genomics, nanotechnology, biosensor, telemedicine and traditional chinese medicine

    中心亦和中文大學理學院及中醫中藥研究所合作，積極參與有關應用基因組、納米技術、生物感應器、遠程醫療以及傳統中醫學等多個獲重點撥款的研究項目。
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