重組基因文庫 的英文怎麼說

中文拼音 [zhòngzǔjīyīnwénkù]
重組基因文庫 英文
library of recombinant
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 文 : Ⅰ名詞1 （字） character; script; writing 2 （文字） language 3 （文章） literary composition; wri...
  • 重組 : bpr
  • 文庫 : a series of books issued in a single format by a publisher; library
  1. In summary, we constructed and characterized a bac library of silky, a unique chinese native breed of chicken. the library has high genome coverage ( 13 - fold ), chimerism ( 6 % ) and overlapped bacs, which made it a valuable resource to complete chicken physical map, study functional genes and construct bac contigs. we analyzed the whole genomic sequence and snps of tyrp1 and found that silky tyrp1 is different with other breeds both in microsatellite and transcription regulation site

    中國農業人學博卜學位論文摘要本研究構建了中國特有雞種絲羽鳥骨雞基因組bac文庫並進行了文庫質量鑒定，它所具有的13倍高基因組覆蓋率、 6 %的嵌合率和部分重疊的克隆使其成為完善雞的基因組圖譜、研究基因功能和構建bac重疊群的優質資源。
  2. At first, this thesis analyzed some essential elements about the system of personal houe loan and make the compare to chinese and foreign system, and established the system of personal credit evaluate ; the second, the thesis discusses the investment technique and strategy of national debt in the provident fund, and established the model about how to invest the national debt ; the third, the thesis build the forecast model about fund collecting and drawing, and make use of the combination invest theories to build model of individual loan and national debt ; at last, the thesis analyses the risk ' s inside reason of house funds with the risk type, and to give out the related suggestion to funds risk. mechanism. the thesis research show me how to make use of that some models and methods in the process of haf management and make me deeply understand the house funds

    本文首先分析了個人住房貸款制度基本要素，即貸款期限、貸款利率與抵押物價值的比例、政府在個人住房貸款市場中的作用、貸款違約情況下的處置措施、個人住房貸款的流動性問題，並對中外製度作了比較，建立了個人信用評分評級體系和信用評估模型，並以重慶市住房公積金為研究對象做出了住房資金個貸風險評估的實證研究；其次，分析了影響國債價格走勢的因素，討論了公積金國債的投資技巧和策略，並建立了基於理論的國債投資組合模型；接下來，根據資產負債管理理論中的資金總庫法和資金分配法分析了公積金總體資金項目的來源和運用，並就此作了總量平衡模型，對住房公積金季度累計歸集金額作了直線回歸和季節趨勢比率預測，運用投資組合理論建立了公積金個人貸款和國債投資組合的最優化模型；最後，探析了住房資金風險的內在原因和風險類型，從資金籌集風險、信貸回歸風險、保險機制、法律風險和政策風險五個方面為住房資金風險防範機制建設提出了相關建議。
  3. The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga. - galactosidase assay indicated that got did not have the property of self - activation. after pgbkt7 - ga was transformed to yeast pj69 - 4a, we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga

    通過pcr擴增得到gpa1基因並將其克隆到雙雜交dna結合域載體pgbkt7中，得到的重組質粒命名為pgbkt7 - g ， ?半乳糖苷酶活性鑒定表明g不具有自激活特性，將其轉化到酵母菌pj69 - 4a中，再以此為受體菌轉化擬南芥綠色營養組織cdna文庫質粒。
  4. In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer

    應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。
  5. The library consisted of 1. 3 x 106 clones with an average insert size of about 18kb. the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin. screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr, we got four positive clones

    3x10 『個重組噬菌體，插入片段大小約為18kb ，含插入片段的頻率為100隊以中亞濱蓉甜菜堿醛脫氫酶門adh ）基因近5 』端的約400hp片段為探針，篩選中亞濱蓉基因組文庫，得到了4個陽性克隆。
  6. Expressed sequence tag ( est ) analysis was carried out to study the molecular mechanism of salt tolerance for polygonum sibiricum laxm. a cdna library was constructed from polygonum sibiricum laxm. treated by salt using stratagene cdna synthesis & gigapack iii gold cloning kit

    為了研究西伯利亞蓼耐鹽的分子機理，使用stratagene的cdna合成和gigapack gold包裝試劑盒構建了鹽（ nahco _ 3 ）脅迫下西伯利亞蓼的cdna文庫，隨機選取重組克隆測序，用表達序列標簽（ est ）方法研究鹽脅迫下西伯利亞蓼基因的表達。
  7. If the technicians applied technology in the wrong, even achieving the business logic, but probably leading to many vices including low performance, low scalability, close coupling, low software duplication. so how to assemble the j2ee technology reasonable and achieve a system with high performance and high expansibility is my research emphasis in the thesis. in order to solve the problems and implement efficient web application, the paper put forward ejs _ mvc model according mvc pattern and we can assemble component by using ejs _ mvc model, it can solve many problems of web application and improving system with clear flow and clear function partition, in addition, due to controller is the most importance in the ejs _ mvc model, so the thesis also discuss some problems about controller design ; if the model is not designed accurately, system performance will encounter fatal influence, so the article lucubrate ejb technology from ejb choice, ejb optimized design, database access, design pattern etc and bring forward some strategies and methods about how to build efficient business tier ; finally based on ejs _ mvc model, a example that contains simple business logic is developed according to the object - orient software engineering thinking and some strategies and methods proposed by the thesis, in the process of achieving system function, the emphasis is probing into how t o assemble and apply technology reasonable and providing a new thinking thread and method contributing to build high effective and flexible j2ee application

    由於j2eeweb應用是由組件組成，因此為了解決上述問題，實現高效的應用，本文首先從如何合理組合組件入手，找到一種方法使各組件能具體分工而又緊密合作，在深入研究各組件基礎上，根據mvc模提出了ejs _ mvc模型概念，指出可按此模型組合各層組件，該模型可以解決傳統web開發中存在的問題，而且具有系統流程與系統功能劃分清晰，可擴展性、可維護性強等優點，另外由於控制器是ejsmvc模型的重中之重，它起到承上啟下的作用，它設計好壞直接關繫到整個應用的性能、伸縮性與擴展性，因此又探討了控制器設計的有關問題；另外如果模型設計不當的話，對系統性能造成的影響可能是致命的，因此本文又從ejb組件選擇、 ejb調優設計、數據庫訪問和設計模式等方面對ejb技術作了全面的分析研究，指出在業務層中如何避免太多網路遠程調用和提高業務層性能，特別是根據前面的分析總結出了業務層的優化分層組合模型，這個模型的使用無疑會使業務層具有較高的性能與伸縮性；最後選擇一個業務邏輯較簡單的系統，使注意力集中到運用的j2ee技術上來，按照ejs _ mvc模型與軟體工程流程以及本論文所提出的方法與策略實現業務邏輯，在實現過程中具體探討如何合理運用組合技術，就多層j2ee體系結構的設計思想作深入的探討實踐，為實現高效、靈活的多層j2ee應用提供一種新的思路及方法。
  8. It is used as the bait to screen azospirillum brasilense sp7 genomic plasmid library which was constructed by fusing 0. 5 - 3kb fragments of a. brasilense sp7 genomic dna to the dna - activation domain in the pgad plasmid vectors

    Brasilensesp7的nifa全序列構建在pgbd - c2載體上，得到重組質粒pgbd - nifa ，以其為誘餌，篩選sp7質粒基因組文庫（構建在pgad系列質粒上） 。
  9. It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified

    中國豬瘟兔化毒（脾淋毒）基因組cdna文庫的構建、序列分析：根據已發表的豬瘟病毒（ csfv ）核苷酸序列，藉助計算機軟體分析，選擇高保守區段和基因組中的單一限制性酶切位點，利用rt - pcr及nested - pcr和helf - nestedpcr技術，成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ，分別克隆到pmd - 18t或pgem - teasy載體進行測序后，拼接出了其核苷酸序列。
  10. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  11. Depending on the degenerate primers which were designed according to the conserved amino acids of glycine betaine secondary transporter, a fragment about 1 kb was obtained by pcr. the pcr fragment was purified and labeled with dig as a probe

    將其總dna用sau3ai部分酶切后，收集4 15kb大小的酶切片段，克隆到用bamhi酶切的puc18中，構建halobacillussp . d8基因組文庫，共獲得約9000個重組質粒。
  12. We make use of object, property, method in asp to complete many functions, including browser splitint many pages in query section and count vistor. we research the relation between server and score data with ado. compared sql language with object, property, method in asp, the result is we find out a combination method of them. we design, the ssms based on browser / server structure

    因此，本文重點探討了如何利用asp技術的內置對象、 activex服務器組件和ado對象來實現對學生成績數據庫的訪問方法，給出了設計細節和具體步驟和方法基於b / s結構的學生學籍管理系統（以下簡稱ssms ）就是利用b / s技術和基於web的應用系統開發相結合實現的，它具有數據輸入、查詢修改、補考處理、畢業處理和報表輸出等功能。
  13. Through in situ hybridization and colony pcr, a positive recombinant plasmid was isolated from the genomic library and sequenced. the inserted dna fragment was about 4. 3 kb

    將pcr擴增片段用地高辛標記探針，利用原位雜交和菌落pcr從基因文庫中獲得含有陽性信號的重組質粒。
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