重組株 的英文怎麼說

中文拼音 [zhòngzǔzhū]
重組株 英文
recombinant strain
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 株 : Ⅰ名詞1. （露在地面上樹木的根和莖） root and stem of a tree above the ground 2. （植株） individual plant; plant Ⅱ量詞（棵）
  • 重組 : bpr
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank

    以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。
  3. It ' s very essential to quicken the development of modern logistics for the renewal of the enterprise ' s management concepts, the promotion of its reform, the improvement of the distribution of materials, the acceleration of the recombination assembled compages compounding fit together resultant weave of the traditional transportation, storage, information and industry, and the enhancement of the economy quality, etc. this paper puts emphasis on the discussion of the essentiality and possibility of building a logistics center in zhuzhou, the scientific consideration of the primary programming and planning of the construction of the regional logistics center in zhuzhou and its information - platform, and the prospect of this center ' s favorable social and economic effect by starting with the introduction of the basic theory about the regional logistics center and combining the modern basic logistics theories and management ideas with the investigations of the social and economic status quo and development in zhuzhou

    加快現代物流的發展，對于更新企業的管理理念，促進企業改革，優化資源配置，促進傳統運輸、倉儲、信息及生產產業的重組，提高經濟運行質量等均具有十分重要的作用。本文從對物流及區域物流中心的基本理論研究入手，通過現代物流基本理論與管理思想，結合開展株洲地區社會經濟現狀與發展調查，重點論述了建設株洲區域物流中心的必要性與可行性，科學思考了建設株洲區域物流中心的初步規劃及現代物流中心的信息平臺的規劃建設，展望了株洲區域物流中心的良好社會效益和經濟效益。
  4. According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified

    以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  7. Swine is the only animal which can be infected by either avian or human original influenza viruses, and c an be considered as the intermediate host for the process of genetic reassortments between viruses of different hosts

    此外，豬是禽和人流感病毒均可感染的唯一動物，是不同來源流感病毒發生重組，產生新的流感毒株的中間宿主。
  8. To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions

    在相同的培養條件下比較了mut ~ +和mut ~ s重組菌株表達pap的異同。
  9. Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector

    本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。
  10. The ginger ( zingiber officinale ) plantlets cultured in triangle vessels under photoautotrophic micropropagation were vigorous and 1. 4, 1. 5, 1. 7 times in leaf number, dry weight, and stem elongation compared to those culutred under conventional micropropagation, respectively. 5. some improved suggestions were shown based on the disadvantages of the environmental control system in the closed tissue culture system

    利用該環境控制系統，在三角瓶容器中採用光獨立組織培養的生薑組培苗長勢粗壯、品質高，其新增葉片數、干鮮重和株高的增加分別為常規組織培養的1 . 4 ， 1 . 5和1 . 7倍。
  11. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  12. 3. the effect of sporulation - independent promotor on toxicity of natural strain in order to study the effect of sporulation - independent promotor ( p3a ), p3a was spliced with the cry1c gene, then inserted into the shuttle vector pht304, and then recombinated plasmid pbmb827 was obtained. after transferring pbmb827 into strain ybt - 1520, it was surprising that the transformants had almost no potency against all lepidopteran larvae tested

    3非依賴芽胞形成icp的cry3a啟動子（ p _ （ 3a ） ）對野生菌株特性的影響帶p _ （ 3a ）和cry1c基因的重組質粒pbmb827轉入ybt - 1520 ，轉化子對所測昆蟲的毒力下降非常明顯，芽胞和晶體也很難脫落。
  13. Sequencing analysis showed that the sequence of endoglucanase fragment exhibits 35 % homology with b. subtil is chromosomal dna ( from glyb to apre ), and 27 % homology with bacillus sp

    將此重組質粒phchi轉化巨大芽抱桿菌b . megateriumap25 ，得到兩株轉化子，分別命名為p25113一9 ， p25113一10 。
  14. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  15. Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing, while a faint band only in muts recombinant after 72 hours. western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced. anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them

    結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。
  16. The purpose of this study was to clone the major structural protein vp3 gene of gpv hl isolate after pcr, and express by protokaryotic and eukaryotic expressing system, then develop molecuiar diagnostic reagent of goose piaque and construct recombillat fowlpox vina life vector vaccine

    利用pcr方法擴增和克隆gpvh1分離株主要免疫原性蛋白基因vp3 ，並對其進行原核和真核表達，是建立小鵝瘟分子診斷方法、構建vp3基因重組禽痘病毒活載體疫苗的基礎，具有極為重要理論和實踐意義。
  17. 97 % identities in amino acids respectively. the e. coli strain dh5 transformed recombinant plasmid phn was induced with 0. 6 mmol / m iptg for n gene expression. the expressed product was identified by sds - page and westem - blot test, a fusion protein about 47ku as we expected was found

    將含有重組質粒phn的菌株dh5在37條件下培養，以濃度為0 . 6mmol / liptg誘導，重組質粒n基因phn融合蛋白獲得了表達：經sds - page ， western - blot試驗，確定其表達的融合蛋白產物大小為預期的47ku 。
  18. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  19. Then the plasmid was extracted from them and determined by dna calculator. the protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1 % glutaral, pay it to electric stimulation in muscle of injection site and extract omni - rna from injection site of rabbit muscle, expression of grf were detected by reverse transcription and pcr ; ratio of expression of grf were detected by elisa. extract dna form injection site of mouse muscle to research time of expression

    本實驗是將含grf重組質粒的jm109菌株大量培養，用堿裂變法提取質粒，用dnacaculator定量；制備含grf的原生質體，經1的戊二醛處理后注射於家兔肌肉，在注射部位給予電刺激，提取總rna ，用rt - pcr檢測grf在肌肉中的表達；用elisa法定性檢測grf在肌肉中蛋白質水平的表達；將該原生質體注射于小鼠肌肉中，定期提取dna ，初步探討原生質介導的外源性grf在小鼠肌肉中的表達時間。
  20. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
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